Description
Gentle and easy-to-use kit allows to isolate flow-compatible human CD4+ T-cells from PBMC or directly from whole blood
Immediately after positive isolation, beads are released and removed from cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column. Purity level of >95% and up to 75% recovery of human CD4+ cells.
- No risk of your results being affected by the isolation method
- Gentle tube-based method - no columns needed
- High purity and recovery
- Bead-free cells - compatible with flow cytometry analysis
- Viable, bead-free CD4+ T-cells for direct use in any cell-based assay
- Capacity: Processes approx. 2x109 cells total
- Cell Type: T Cells (CD4)
- Isolation Method: Positive Isolation
- Output Viability: >95%
- Sample Type: Blood, PBMC
- Starting Material (Cell No.): 5x107 PBMCs or 2mL whole blood per isolation
- Target Species: Human
- Starting sample:MNC or whole blood
- Capacity: The kit will process 2 x 109 mononuclear cells (MNC) or 80mL whole blood
Cell Analysis, Cell Isolation, Cell Isolation and Expansion, Human Cell Isolation