Description
Thermo Scientific™ Fermentas Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes.
The kit is optimized to reproducibly provide high sensitivity with a low background in applications such as Southern, Northern, dot and slot blotting, as well as screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods including the Biotin DecaLabel DNA Labeling Kit. Biotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP). Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes. The probes then are visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate. Therefore, visualization does not require X-ray film or other specific equipment (Fig. 1). The Biotin Chromogenic Detection Kit includes Blocking reagent and Blocking/Washing buffers which are specifically designed to minimize background, which is often associated with biotin detection in conventional systems.The positively charged SensiBlot Plus Nylon Membrane is a recommended component of this detection system to ensure the highest sensitivity of detection and the lowest background.
- High sensitivity of detection – 0.03pg of homologous DNA in dot blot hybridization with biotin-labeled probe
- Low background due to optimized washing and blocking procedures
- Non-radioactive detection visualization does not require X-ray film or other specific equipment
- Fast and convenient – ready-to-use components
Recommended for: Southern, Northern, dot/slot blots, plaque or colony screening