Description
Kit combines the convenience of spin column technology with the selective binding properties of a silica membrane, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
- Developed as 3 in 1 kit designed for rapid and efficient purification of DNA from PCR, enzymatic reaction mixtures, and DNA extraction from standard or low-melting point agarose gels run in either Tris acetate (TAE) or Tris borate (TBE) buffer
- Kit combines the convenience of spin column technology with the selective binding properties of a silica membrane
- This eliminates the need for tedious resin manipulations or toxic phenol-chloroform extractions
- It effectively removes primers, primer dimers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures
- The kit can be used for purification of DNA fragments from 100 bp to 20 kb
- Recovery rates are 90 to 100% for 100 bp to 3 kb DNA fragment size range
- Each GeneJET DNA Purification Column has a total binding capacity of up to 10 µg of DNA
- The entire procedure takes approximately 3.5 minutes for DNA cleanup
Highlights - Advanced column design – easy to open, load, and handle
- Yields concentrated DNA – Elute in 6 to 10 µL volume
- High recovery – 90 to 100% recovery in the 100 to 3000 bp DNA fragment range
- Fast – 4 minute reaction mixture clean-up and 15 minute agarose gel purification protocols available
- Efficient removal of reaction contaminants – including enzymes, proteins, primers dimers, dNTPs, and salts
- Flexible – does not require precise gel slice quantification weighting
Applications - Sequencing, Restriction digestion, PCR, qPCR, Labeling, Ligation, Cloning, In vitro transcription, Blotting, In situ hybridization
Includes - Binding Buffer, Extraction Buffer, Prewash Buffer (conc.), Wash Buffer (conc.), Elution Buffer, and DNA Purification Micro Column & Collection Tube