Fig 1: Efficiency of DNA removal by DNaseI and its fusion variants during RNA purification procedure.DNaseDT denotes the fusion with the (HhH)2 domain of DNase from an extremely salt tolerant bacterium Thioalkalivibrio sp. K90mix, DNaseBS denotes the fusion with homologous domain of ComEA protein from Bacillus subtilis. The upper picture represents quantitative evaluation of undigested DNA remaining in eluates (RT-qPCR without added reverse transcriptase). The lower picture represents RT-qPCR results obtained using the same eluates. In the both pictures labels on the horizontal axis indicate the used nuclease, amount of it and the dilution ratio of the eluates before reverse transcription step and quantitation of DNA. A modified protocol of GeneJET™ Whole Blood RNA Purification Mini Kit (Thermo Fisher Scientific, #K0761) was followed and RNA purification columns supplied by the manufacturer were used. During the experiment we have purified total blood RNA. Three arbitrary blood samples were analysed. DNA digestion was performed directly on a filter of a RNA purification column, were respective DNase enzyme was loaded.