Octet® Protein A (ProA) Biosensors 18-5010, 18-5012, 18-5013 from Sartorius

Product Specs
ItemOctet® Protein A (ProA) Biosensors
CompanySartorius
Price Supplier Page View Company Product Page
Catalog Number18-5010, 18-5012, 18-5013
ApplicationsQuantitation of IgG’s of various species including human
QuantityOne / Five / Twenty trays of 96 biosensors coated with Protein A (recommended Sample Diluent sold separately)

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Local Office

Sartorius

5 Orville Drive

Bohemia, NY 11716

United States

Company Profile

International Office

Sartorius

Otto-Brenner-Straße 20

37079 Göttingen

Germany

Phone: +49 551 308 0

Company Profile

Website: www.sartorius.com

(1) Rapid, high throughput, automated detection of SARS-CoV-2 neutralizing antibodies against Wuhan-WT, delta and omicron BA1, BA2 spike trimersiScienceNovember 17, 2023Narayanaiah Cheedarla, Hans P. Verkerke, Sindhu Potlapalli, Kaleb Benjamin McLendon, Anamika Patel, Filipp Frank, William Henry O’Sick, Suneethamma Cheedarla, Tyler Jon Baugh, Gregory L. Damhorst, et al.,Thermo Fisher Scientific,Cat#23227 Anti-His Biosensors,ForteBio,Cat#18-5114 ProA Biosensors,Sartorius,Cat#18-5010 "EDAC, 1-Ethyl-3-(3-Dimethylaminopropyl)carbodiimide, Hydrochloride",Thermo Fisher Scientific,Cat#E2247 Streptavidin-β-galactosidase

(2) Broadly neutralizing humanized SARS-CoV-2 antibody binds to a conserved epitope on Spike and provides antiviral protection through inhalation-based delivery in non-human primatesPLoS PathogAugust 2, 2023Paule Hermet, Benoît Delache, Cecile Herate, Esther Wolf, Gaily Kivi, Erkki Juronen, Karl Mumm, Eva Žusinaite, Denis Kainov, Eve Sankovski, et al.nM, 50 nM, 25 nM, 12.5 nM, and 6.75 nM. Octet Protein A (ProA) Biosensors (Sartorius, catalog number: 18–5010) were incubated for at least 10 min in 200 μl kinetics buffer before the assay. Biosensors were regenerated

(3) Real-Time Immunosensor for Small-Molecule Monitoring in Industrial Food ProcessesAnalytical ChemistryMay 23, 2023Chris Vu, Yu-Ting Lin, Stijn R. R. Haenen, Julia Marschall, Annemarie Hummel, Simone F. A. Wouters, Jos M. H. Raats, Arthur M. de Jong, Junhong Yan, Menno W. J. PrinsOctet measurements (ForteBio Octet Red96) with a Protein A-coated sensor (Molecular Devices, cat. #18-5010) and a hIgG1 standard curve (Sartorius, cat. #18-1118). Recombinant (scFv)2-Fc and (VHH)2-Fc Antibody

(4) Modular adjuvant-free pan-HLA-DR-immunotargeting subunit vaccine against SARS-CoV-2 elicits broad sarbecovirus-neutralizing antibody responsesCell reportsApril 25, 2023Kassardjian A, Sun E, Sookhoo J, Muthuraman K, Boligan KF, Kucharska I, Rujas E, Jetha A, Branch DR, Babiuk S, et al.commercial assays,Critical commercial assays ,, Octet® Protein A (ProA) Biosensors,Sartorius,Cat#18-5010 Octet® Anti-Penta-HIS (HIS1K) Biosensors,Sartorius,Cat#18-5120 4X TaqMan Fast Virus one step RT-PCR kit

(5) Bispecific antibodies combine breadth, potency, and avidity of parental antibodies to neutralize sarbecovirusesiScienceApril 21, 2023Laura Radić, Kwinten Sliepen, Victor Yin, Mitch Brinkkemper, Joan Capella-Pujol, Angela I. Schriek, Jonathan L. Torres, Sandhya Bangaru, Judith A. Burger, Meliawati Poniman, et al.GE29-0915-96 Octet K2 system,Sartorius (FortéBio),N/A Octet Biosensors: Protein A,Sartorius (FortéBio),Cat# 18-5010 Octet Biosensors: NiNTA,Sartorius (FortéBio),Cat# 18-5101 Nucleobond Xtra Maxi kit,Macherey-Nagel,Cat#

(6) Engineering of a Bispecific Nanofitin with Immune Checkpoint Inhibitory Activity Conditioned by the Cross-Arm Binding to EGFR and PDL1BiomoleculesMarch 31, 2023Perrine Jacquot, Javier Muñoz-Garcia, Maurine Fleury, Denis Cochonneau, Rémi Gaussin, Elise Enouf, Caroline Roze, Emilie Ollivier, Mathieu Cinier, Dominique HeymannRennes, France, #344-ER-050) (10 µg/mL) at 2 nm on protein A biosensors (Sartorius, Dourdan, France, #18-5012). Similarly, the binding kinetic parameters of anti-PDL1 Nanofitins were determined by the loading of

(7) Computational pipeline provides mechanistic understanding of Omicron variant of concern neutralizing engineered ACE2 receptor trapsStructureMarch 2, 2023Soumya G. Remesh, Gregory E. Merz, Axel F. Brilot, Un Seng Chio, Alexandrea N. Rizo, Thomas H. Pospiech, Irene Lui, Mathew T. Laurie, Jeff Glasgow, Chau Q. Le, et al.biosensors (WT-ACE2, CVD293) (Sartorius®, Item no.: 18-5019) or ProtA biosensors (Sartorius®, Item no.: 18-5010) and WT, Delta-, Omicron-SARS-CoV-2 spike RBD are present as the analyte in solution in a 384-well microplate.

(8) Co-display of diverse spike proteins on nanoparticles broadens sarbecovirus neutralizing antibody responsesiScienceDecember 22, 2022Mitch Brinkkemper, Tim S. Veth, Philip J.M. Brouwer, Hannah Turner, Meliawati Poniman, Judith A. Burger, Joey H. Bouhuijs, Wouter Olijhoek, Ilja Bontjer, Jonne L. Snitselaar, et al.,BIO RAD,N/A Octet K2 system,Sartorius (FortéBio),N/A Octet Biosensors: Protein A,Sartorius (FortéBio),Cat# 18-5010 "Vivaspin 20, 100.000 kDa MWCO, Polyethersulfone",Sigma-Aldrich,Cat# GE28-9323-63 Nucleobond Xtra Maxi

(9) SARS-CoV-2 spike protein variant binding affinity to an angiotensin-converting enzyme 2 fusion glycoproteinsPLOS ONEDecember 6, 2022Alicia M. Matthews, Thomas G. Biel, Uriel Ortega-Rodriguez, Vincent M. Falkowski, Xin Bush, Talia Faison, Hang Xie, Cyrus Agarabi, V. Ashutosh Rao, Tongzhong Jucharacterize cell viability via trypan blue staining and protein titer via Protein A biosensors (Cat# 18–5010, Sartorius, Germany) and an Octet 96e (Sartorius) as described by the manufacturer. Purification of

(10) Computational pipeline provides mechanistic understanding of Omicron variant of concern neutralizing engineered ACE2 receptor trapsbioRxivAugust 12, 2022Soumya G. Remesh et al.biosensors (WT-ACE2, CVD293) (Sartorius®, Item no.: 18–5019) or ProtA biosensors (Sartorius®, Item no.: 18–5010) and WT, Delta-, Omicron-SARS-CoV-2 spike RBD are present as the analyte in solution in a 384-well microplate.

(11) A calcium-sensitive antibody isolates soluble amyloid-β aggregates and fibrils from Alzheimer’s disease brainBrainJuly 29, 2022Andrew M Stern et al.standard deviation. Biolayer interferometry Antibodies were loaded onto ProA biosensor (Sartorius, 18-5010) to density 3.5 and 1.0 nm using an Octet RED96e (ForteBio). After equilibrium, binding to 1 µM Aβ40

(12) Structural and biochemical mechanism for increased infectivity and immune evasion of Omicron BA.2 variant compared to BA.1 and their possible mouse originsCell researchJuly 1, 2022Xu Y, Wu C, Cao X, Gu C, Liu H, Jiang M, Wang X, Yuan Q, Wu K, Liu J, et al.dimeric human ACE2 (Sino Biological, 10108-H02H) was immobilized on Protein A biosensor (Sartorius, 18-5012) followed by measuring the association and dissociation with Omicron BA.1 (Sino Biological, 40589-V08H26),

(13) Protocol for high-throughput cloning, expression, purification, and evaluation of bispecific antibodiesSTAR ProtocolsJune 17, 2022Danqing Li, Alexander C. Partin, Liangjun Zhao, Irwin Chen, Mark L. Michaels, Zhulun Wang, Fernando Garces, Danyang Gong, Timothy P. RileyDNA fragments,Twist Bioscience,N/A ,, Other,Other,Other ,, Octet ProA biosensors,Sartorius,Cat# 18-5010 ProA magnetic beads,GenScript,Cat# L00695 Kingfisher 96-well plates,Fisher Scientific,Cat# 95040450 Kingfisher

(14) Variable-heavy (VH) families influencing IgA1&2 engagement to the antigen, FcαRI and superantigen proteins G, A, and LScientific reportsApril 20, 2022Ling WL, Su CT, Lua WH, Yeo JY, Poh JJ, Ng YL, Wipat A, Gan SK.were as previously described48–51,57,58,70,76. PpL (Sartorius, Cat: 18–5185), SpA (Sartorius, Cat: 18–5012), and SpG (Sartorius, Cat: 18–18-5083) biosensors were subject to free floating IgA variants to obtain

(15) Two-Component Spike Nanoparticle Vaccine Protects Macaques from SARS-CoV-2 InfectionSSRN Electronic JournalMarch 30, 2022Philip J. M. Brouwer et al.BIO RAD N/A Octet K2 system Sartorius (FortéBio) N/A Octet Biosensors: Protein A Sartorius (FortéBio) Cat# 18-5010 Vivaspin 20, 100.000 kDa MWCO, Polyethersulfone Sigma-Aldrich Cat# GE28-9323-63 Nucleobond Xtra Maxi

(16) More Than Meets the Kappa for Antibody Superantigen Protein L (PpL)Antibodies (Basel)February 11, 2022Wei-Li Ling, Joshua Yi Yeo, Yuen-Ling Ng, Anil Wipat, Samuel Ken-En Ganperformed using PpL (Sartorius, Singapore, Singapore, Cat: 18-5185), SpA (Sartorius, Singapore, Cat: 18-5012), and SpG (Sartorius, Singapore, Cat: 18-18-5083) biosensors to Pertuzumab and Trastuzumab IgG1 variants

(17) Elicitation of broadly protective sarbecovirus immunity by receptor-binding domain nanoparticle vaccinesCellOctober 1, 2021Alexandra C. Walls et al.2020",https://jbloomlab.github.io/dms_variants/dms_variants.globalepistasis.html ,, Other,Other,Other ,, Octet Biosensors: protein A,Sartorius (FortéBio),Cat# 18-5010 Octet Biosensors: ARG2,Sartorius (FortéBio),Cat# 18-5092 EM supplies 300 mesh grids,Ted Pella,Cat#

(18) An end-to-end automated platform process for high-throughput engineering of next-generation multi-specific antibody therapeuticsmAbsAugust 12, 2021Norbert Furtmann et al.quantification of CODV molecules by using ProA biosensors (Sartorius FortéBio, Göttingen, Germany; Cat. No. 18–5013) was performed as follows: 10 µl supernatant on BQ plates were diluted 1:10 with 90 µl D-PBS (Thermo

(19) Two-component spike nanoparticle vaccine protects macaques from SARS-CoV-2 infectionCellMarch 4, 2021Brouwer PJM, Brinkkemper M, Maisonnasse P, Dereuddre-Bosquet N, Grobben M, Claireaux M, de Gast M, Marlin R, Chesnais V, Diry S, et al.,BIO RAD,N/A Octet K2 system,Sartorius (FortéBio),N/A Octet Biosensors: Protein A,Sartorius (FortéBio),Cat# 18-5010 "Vivaspin 20, 100.000 kDa MWCO, Polyethersulfone",Sigma-Aldrich,Cat# GE28-9323-63 Nucleobond Xtra Maxi

(20) Elicitation of Potent Neutralizing Antibody Responses by Designed Protein Nanoparticle Vaccines for SARS-CoV-2CellNovember 25, 2020Walls AC, Fiala B, Schäfer A, Wrenn S, Pham MN, Murphy M, Tse LV, Shehata L, O'Connor MA, Chen C, et al.v10,FlowJo,https://www.flowjo.com ,, Other,Other,Other ,, Octet Biosensors: protein A,Sartorius (FortéBio),Cat# 18-5010 Octet Biosensors: Anti-Penta-HIS (HIS1K),Sartorius (FortéBio),Cat# 18-5120 Octet Biosensors: NTA,Sartorius

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Octet® Protein A (ProA) Biosensors from Sartorius

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Sartorius

Sartorius

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Octet® Protein A (ProA) Biosensors from Sartorius

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