Fig 1: Loss of Pgrmc1 suppresses IL-6 production in macrophages. (A,B) Plasma level of IL-6 (A) and hepatic mRNA levels of IL-1a, IL-1ß, and Tnf (B) in DEN-injected WT and Pgrmc1-null mice. The mRNA expression levels were normalized to that of Rplp0 and expressed relative to the WT group. * p < 0.05 in Student’s t-test. (C,D) Culture supernatant level of IL-6 (C) and mRNA expression levels of IL-1a, IL-1ß, and Tnf (D) in Raw 264.7 cells transfected with Control (Ctrl) or Pgrmc1-siRNA (KD) and treated with or without necrotic debris for 3 h. The mRNA expression levels were normalized to that of Rplp0 and expressed relative to the Ctrl group. * p < 0.05 in one-way ANOVA followed by Tukey’s post hoc test. (E,F) Control (Ctrl) or Pgrmc1-siRNA (KD)-transfected cells were treated with erlotinib (50 µM) and necrotic debris for 8 h. Level of IL-6 (E) and mRNA expression levels of IL-1a, IL-1ß, and Tnf (F) in the supernatant of erlotinib-treated Raw 264.7 cells. The mRNA expression levels were normalized to that of Rplp0 and expressed relative to the Ctrl group. * p < 0.05 in one-way ANOVA followed by Tukey’s post hoc test.
Fig 2: Loss of Pgrmc1 aggravates liver injury while suppressing compensatory proliferation. (A) WT and Pgrmc1-null mice were injected with high-dose DEN (200 mg/kg, i.p.), and their plasma and livers were collected after 48 h. (B) Plasma level of alanine aminotransferase (ALT). (C) Expression of high mobility group box 1 (HMGB1) in the plasma. The expression level was normalized to that of plasma albumin and expressed relative to the WT group. (D) TUNEL staining in the livers of WT and Pgrmc1-null mice. TUNEL-positive cells were counted and expressed relative to the WT group. Scale bar, 100 µm. (E) Ki67 immunostaining in the livers of WT and Pgrmc1-null mice. Ki67-positive cells were counted and expressed relative to the WT group. Scale bar, 100 µm. (F) mRNA expression levels of C-Myc, Cyclin D, and Hgf in the livers of WT and Pgrmc1-null mice. The expression level was normalized to that of Rplp0 and expressed relative to the WT group. * p < 0.05 in Student’s t-test. All full blot images were provided in Supplementary File (Figure S3).
Supplier Page from Biomatik for Mouse Interleukin 6, Il-6 Elisa Kit