Fig 1: Construction of a COVID-19-type proinflammatory model. A. Cell viability was assessed by a CCK-8 assay at 12 and 24 h. B-E. qRT?PCR detection of the mRNA expression of ACE2, AGTR1, TMRRSS2, and ISG15 at 12 and 24 h. F-J. Western blot detection of the protein expression of ACE2, AGTR1, TMRRSS2, and ISG15 at 12 and 24 h. K-L. ELISAs were used to measure IL-6 and MCP-1 levels. The experiments were repeated three times. The significance of three or more groups was compared by one-way ANOVA. Tukey’s test was adopted for postmortem testing. * P < 0.05 vs. control, # P < 0.05 vs. RBD-500.
Fig 2: GH/E2 alleviated inflammation in a COVID-19-type proinflammatory model. BEAS-2B cells were treated with 1000 ng/mL RBD and 200 ng/mL GH, 1 nM E2, or 10 nM Tes simultaneously for 24 h. A-B. ELISAs were performed to measure IL-6 and MCP-1 levels. C-D. MDA and SOD levels were evaluated by ELISA. E. ROS levels were measured by FCM. The experiments were repeated three times. The significance of three or more groups was compared by one-way ANOVA. Tukey’s test was adopted for postmortem testing. * P < 0.05 vs. control, # P < 0.05 vs. RBD.
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