Fig 1: miR-182 promotes M2 polarization of macrophages in vitro. (A–D) Analyses of M2 polarization of wild-type (+/+) or miR-182-KO (-/-) BMDMs after IL4 and IL13 treatment. Shown are CD206 flow cytometry (A, n = 3 independent experiments), CD206 IF analysis (B, n = 3 independent experiments), ELISA of IL-10, TGFß, and IL10/IL-12 ratio (C, n = 4 independent experiments), and qPCR analyses of additional M2 markers (D, n = 3 independent experiments). (E) Effects of miR-182 mimic on M2 polarization of THP1 macrophages after IL4 and IL13 treatment. Shown are CD206 flow cytometry, ELISA of IL10 and IL10/IL12 ratio, and qPCR analyses of additional M2 markers (n = 3 independent experiments). NC, negative control. (F) Effects of miR-182 mimic on M2 polarization of U937-macrophages after IL4 and IL13 treatment. Shown are CD206 flow cytometry, ELISA of IL10 and IL12 secretion, and qPCR analyses of additional M2 markers (n = 3 independent experiments). (G) ARG1 protein expression and STAT3/6 phosphorylation in wild-type or miR-182-KO BMDMs and in THP1- or U937-derived macrophages treated with miR-182-overexpressing mimic or miR-182 oligonucleotide inhibitor (Inh). (H–J) Analysis of M2 polarization of IL4/IL13-activated wild-type BMDMs after treatment of miR-182 inhibitor (Inh) and miR-182-KO BMDMs after miR-182 overexpression. Shown are CD206 flow cytometry (H, n = 3 independent experiments), ARG1 protein expression and STAT3/6 phosphorylation (I), and CD206 IF analysis (J, n = 3 independent experiments). (Scale bar, 100 µm.) P values were obtained by two-tailed unpaired t test. Bar graphs show mean + SD.
Fig 2: HA ameliorates colitis induced by DSS. (A) Experimental design. (B) Percentage change in body weight after DSS treatment. (C) Disease activity index on day 7. (D,E) Representative images and length of the colon. (F,G) Representative image of H&E staining of colon and pathological score. The right lower panels (20×) are images enlarged from the low magnification panels (2.5×). (H) Relative mRNA expression of inflammatory factors in the colon. (I–M) Concentrations of IL4, IL6, TNFa, IL17, and IL10 in serum. Data are expressed as mean ± SE, n = 8–10, * p < 0.05, ** p < 0.01.
Supplier Page from Proteintech Group Inc for Mouse IL-10 ELISA Kit