Nuclei Isolation Kit: Nuclei EZ Prep NUC101 from MilliporeSigma

Product Specs
ItemNuclei Isolation Kit: Nuclei EZ Prep
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberNUC101
Applicationscell analysis
Quantity1KT

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) A versatile method for the identification of senolytic compoundsCell StressDecember 11, 2023Chiara Annunziata, Francesca Castoldi, Schlegel Jan, Hazel X. Ang, Mina Ristovska, Stefania Melini, Robert Welch, Christian G. Riedel, Federico Pietrocolanuclei were isolated from H2B-GFP/H2B-RFP co-cultures using the Nuclei Isolation Kit (Sigma Aldrich, #NUC101) as per manufacturer instructions. Nuclei were resuspended in PBS containing 5mM EDTA and 0.5% BSA and

(2) A primate nigrostriatal atlas of neuronal vulnerability and resilience in a model of Parkinson’s diseaseNature CommunicationsNovember 18, 2023Lei Tang, Nana Xu, Mengyao Huang, Wei Yi, Xuan Sang, Mingting Shao, Ye Li, Zhao-zhe Hao, Ruifeng Liu, Yuhui Shen, et al.(https://www.10xgenomics.com/support/single-cell-gene-expression/documentation/steps/sample-prep/isolation-of-nuclei-for-single-cell-rna-sequencing-and-tissues-for-single-cell-rna-sequencing). In brief, the tissue was lysed via a chilled lysis kit (Sigma, no. NUC101-1KIT). The suspension was then filtered with cell strainer and washed impurities by centrifugation with

(3) Transcriptional linkage analysis with in vivo AAV-Perturb-seqNatureOctober 12, 2023Antonio J. Santinha, Esther Klingler, Maria Kuhn, Rick Farouni, Sandra Lagler, Georgios Kalamakis, Ulrike Lischetti, Denis Jabaudon, Randall J. Platt(Sigma-Aldrich, D8938) was filled with 2 ml of ice-cold nucleus isolation buffer (NIB) (Sigma-Aldrich, NUC101-1KT) and frozen pieces of tissue were directly placed inside the grinder. For all of the experiments,

(4) Decoding transcriptomic signatures of Cysteine String Protein alpha-mediated synapse maintenancebioRxivOctober 10, 2023Na Wang, Biqing Zhu, Mary Alice Allnutt, Rosalie M. Grijalva, Hongyu Zhao, Sreeganga S. Chandralittermate mice were gently Dounce homogenized in 2 ml of ice-cold Nuclei EZ Prep buffer (Sigma, Cat.NUC101–1KT) with a loose pestle, and then with a tight pestle each for 25 times. The resulting homogenate was

(5) Cell type-specific gene expression dynamics during human brain maturationbioRxivOctober 6, 2023Christina Steyn, Ruvimbo Mishi, Stephanie Fillmore, Matthijs B. Verhoog, Jessica More, Ursula K. Rohlwink, Roger Melvill, James Butler, Johannes M. N. Enslin, Muazzam Jacobs, et al.dounce-homogeniser containing 2 ml ice-cold lysis solution (Nuclei EZ Lysis Buffer [Sigma-Aldrich, NUC101] or Nuclei PURE Lysis buffer [Sigma-Aldrich, NUC201] with 1 mM dithiothreitol [DTT, Promega, P1171,

(6) Tracking cell-type-specific temporal dynamics in human and mouse brainsCellSeptember 28, 2023Lu Z, Zhang M, Lee J, Sziraki A, Anderson S, Zhang Z, Xu Z, Jiang W, Ge S, Nelson PT, et al.a 1:1 ratio, washed with ice-cold PBS, and lysed in 1 mL ice-cold EZ lysis buffer (Millipore Sigma, NUC101). The nuclei were then fixed on ice with 1% formaldehyde (Thermo Fisher Scientific, 28906) for 10 min

(7) Single-cell analysis reveals region-heterogeneous responses in rhesus monkey spinal cord with complete injuryNature communicationsAugust 9, 2023Fan Y, Wu X, Han S, Zhang Q, Sun Z, Chen B, Xue X, Zhang H, Chen Z, Yin M, et al.homogenized on ice using a glass Dounce tissue grinder (D8938; Sigma) in 1 ml of cold Nuclei EZ lysis buffer (NUC101-1KT; Sigma). Each sample was ground 15 strokes with pestle A, followed by 15 strokes with pestle B.

(8) Rhenium(I) Tricarbonyl Complexes of 1,10-Phenanthroline Derivatives with Unexpectedly High CytotoxicityInorganic ChemistryAugust 7, 2023Lucy E. Enslin, Kallol Purkait, Maria Dalla Pozza, Bruno Saubamea, Pierre Mesdom, Hendrik G. Visser, Gilles Gasser, Marietjie Schutte-SmithICP-MS. Each experiment was performed in triplicate. Nuclei: The nuclei isolation kit, Nuclei EZ Prep (NUC101) (Sigma-Aldrich) was used to isolate the nuclei from the complex-treated cells, and the standard kit

(9) Spatial and single-nucleus transcriptomic analysis of genetic and sporadic forms of Alzheimer’s DiseasebioRxivJuly 30, 2023Emily Miyoshi, Samuel Morabito, Caden M. Henningfield, Negin Rahimzadeh, Sepideh Kiani Kiani Shabestari, Sudeshna Das, Neethu Michael, Fairlie Reese, Zechuan Shi, Zhenkun Cao, et al.samples. 50mg fresh frozen postmortem human brain tissue was homogenized in Nuclei EZ Lysis buffer (NUC101–1KT, Sigma-Aldrich) and incubated for 5 min before being passed through a 70μm filter. Samples were

(10) TrkB-dependent regulation of molecular signaling across septal cell typesbioRxivJuly 7, 2023Lionel A. Rodriguez, Matthew Nguyen Tran, Renee Garcia-Flores, Elizabeth A. Pattie, Heena R. Divecha, Sun Hong Kim, Joo Heon Shin, Yong Kyu Lee, Carly Montoya, Andrew E. Jaffe, et al.previously described 32,33. Tissue was homogenized in chilled nuclei EZ lysis buffer (Millipore Sigma #NUC101) using glass dounce with ~15 strokes per pestle. Homogenate was filtered, centrifuged and resuspended

(11) Targeting NETs using dual-active DNase1 variantsFrontiers in ImmunologyMay 23, 2023Hanna Englert, Josephine Göbel, Danika Khong, Maryam Omidi, Nina Wolska, Sandra Konrath, Maike Frye, Reiner K. Mailer, Manu Beerens, Julian C. Gerwers, et al.DNase1L3 activity we isolated nuclei from HEK293 cells using the Nuclei Isolation Kit Nuclei EZ Prep (NUC101, Sigma). 5 µl of supernatants from DNase1 mutants expressing HEK293 cells, murine serum, urine, or bile

(12) A druggable copper-signalling pathway that drives inflammationNatureMay 1, 2023Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD, et al.mitochondrial pellets. Isolation of nuclei Nuclei were isolated using the Nuclei EZ Prep (Sigma-Aldrich, NUC101-1KT) according to the manufacturer’s instructions. In brief, cells were treated as indicated and collected

(13) Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and TranscriptomeJournal of Visualized ExperimentsMarch 13, 2023Sarah R. Ocañas, José V. V. Isola, Tatiana D. Saccon, Kevin D. Pham, Ana J. Chucair-Elliott, Augusto Schneider, Willard M. Freeman, Michael B. StoutLaboratory,29899 Cyp17iCre mouse model,The Jackson Laboratory,28547 Nuclei Isolation Kit: Nuclei EZ Prep,Sigma-Aldrich,Nuc101 1 M HEPES,Gibco,15630-080 5 M NaCl,Thermo Scientific,AM9760G 2M KCl,Thermo Scientific,AM9640G 0.5

(14) N-acetylneuraminic acid links immune exhaustion and accelerated memory deficit in diet-induced obese Alzheimer’s disease mouse modelNature CommunicationsMarch 9, 2023Stefano Suzzi, Tommaso Croese, Adi Ravid, Or Gold, Abbe R. Clark, Sedi Medina, Daniel Kitsberg, Miriam Adam, Katherine A. Vernon, Eva Kohnert, et al.lysis buffer. Lysis buffer used was either EZ Lysis Buffer, as we previously used100 (Sigma-Aldrich, NUC101), or Igepal Lysis Buffer, containing 0.1% IGEPAL® CA-630 (Sigma-Aldrich, I8896), 10 mM Tris HCL pH 7.5,

(15) The molecular consequences of androgen activity in the human breastCell GenomicsMarch 8, 2023Florian Raths, Mehran Karimzadeh, Nathan Ing, Andrew Martinez, Yoona Yang, Ying Qu, Tian-Yu Lee, Brianna Mulligan, Suzanne Devkota, Wayne T. Tilley, et al.proteins","Chemicals, peptides, and recombinant proteins" ,, Nuclei EZ Lysis Buffer,Sigma-Aldrich,Cat#NUC101-1KT RiboLock RNase Inhibitor (40 U/μL),Thermo-Fisher Scientific,Cat#EO0381 Nonidet P40 Substitute,Sigma-Aldrich,Cat#11754599001 Digitonin (5

(16) Platelet-instructed SPP1(+) macrophages drive myofibroblast activation in fibrosis in a CXCL4-dependent mannerCell reportsFebruary 28, 2023Hoeft K, Schaefer GJL, Kim H, Schumacher D, Bleckwehl T, Long Q, Klinkhammer BM, Peisker F, Koch L, Nagai J, et al.porcelain mortars and subsequently homogenized in 500 μL nuclei lysis buffer (NLB, EZ lysis Buffer, NUC101, Sigma-Aldrich plus Protector RNAse Inhibitor, Roche) using glass tissue grind tubes and pestles. Homogenized

(17) eRNA profiling uncovers the enhancer landscape of oesophageal adenocarcinoma and reveals new deregulated pathwayseLifeFebruary 20, 2023Ibrahim Ahmed, Shen-Hsi Yang, Samuel Ogden, Wei Zhang, Yaoyong Li, Andrew D The OCCAMs consortium, Andrew D The OCCAMs consortiummodifications. Briefly, the nuclei were prepared from 3 million cross-linked cells (−80°C) using Nuclei EZ prep (NUC101, Sigma-Aldrich) at 4°C for 10 min and spun down 500 × g at 1°C for 5 min. The nuclei wash was carried

(18) HIV viral protein R induces loss of DCT1-type renal tubulesbioRxivFebruary 7, 2023Khun Zaw Latt, Teruhiko Yoshida, Shashi Shrivastav, Amin Abedini, Jeff M. Reece, Hewang Lee, Koji Okamoto, Jurgen Heymann, Yongmei Zhao, Joon-Yong Chung, et al.of Kirita et al.15 Briefly, ~ 8 mm3 tissue fragments were cut by razor blade in the EZlysis buffer (#NUC101–1KT, Sigma, Darmstadt, Germany) and homogenized 30 times using a Dounce homogenizer equipped with a

(19) Single-nuclei transcriptome analysis of Huntington disease iPSC and mouse astrocytes implicates maturation and functional deficitsiScienceJanuary 20, 2023Andrea M. Reyes-Ortiz, Edsel M. Abud, Mara S. Burns, Jie Wu, Sarah J. Hernandez, Nicolette McClure, Keona Q. Wang, Corey J. Schulz, Ricardo Miramontes, Alice Lau, et al.timepoint were isolated from half hemisphere striatum or cortex in Nuclei EZ Lysis buffer (Sigma-Aldrich, NUC101-1KT) and incubated for 5 minutes. Samples were passed through a 70 μm filter and incubated in additional

(20) Rapid nuclear deadenylation of mammalian messenger RNAiScienceJanuary 20, 2023Alles J, Legnini I, Pacelli M, Rajewsky N.assays,Critical commercial assays,Critical commercial assays ,, Nuclei EZ Prep kit,Sigma Aldrich,Cat#NUC101-1KT USB poly(A) length assay kit,Thermo Fisher,Cat#764551KT TruSeq mRNA preparation kit,Illumina,Cat#RS-122-2102 SMARTScribe

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Nuclei Isolation Kit: Nuclei EZ Prep from MilliporeSigma

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Nuclei Isolation Kit: Nuclei EZ Prep from MilliporeSigma

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