Fig 1: OVV-CD19BiTE-mediated oncolysis enhances T-cell function and induces a T-cell-mediated bystander effect.A Human T cells were co-cultured with or without K562, K562/CD19, or Raji cells, respectively, in the presence of the OVV supernatants (100 μl) or blinatumomab (100 ng/ml) for 24 h. Then, average concentration values of IFN-ɣ, TNF-α, and IL-2 cytokines in the supernatants obtained from this co-culture system were measured by ELISA assay. B Preactivated T cells were co-cultured with the indicated target cell lines (E:T = 5) in the presence of the OVV supernatants (100 μl) or blinatumomab (100 ng/ml). The percentage of cytotoxicity of target cells (CFSE+/7AAD+) was assessed by flow cytometry after 48 h co-culture. C CD19BiTE-mediated bystander tumor cell killing. CFSE-stained K562 cells or K562/CD19 were culture in the presence of T cells (E:T = 5), then the indicated supernatants (mock, OVV, OVV-CD19BiTE) and blinatumomab were added to this co-culture system. After 48 h, cytotoxicity of K562 cells and K562/CD19 cells were evaluated by flow cytometry. D Preactivated T cells were co-cultured with the indicated target cell lines (E:T = 5) in the presence of the OVV-CD19BiTE or OVV (MOI = 10) or blinatumomab (100 ng/ml). The percentage of cytotoxicity of target cells (CFSE+/7AAD+) was assessed by flow cytometry after 48 h co-culture. A representative result of triplicates is shown. ***P < 0.001; ns no significance, using one-way ANOVA test with post-hoc analysis.
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