Fig 1: Visfatin induces IL-6 expression in human NP cells. (a-c, e-g) Western blot and RT-PCR analysis of IL-6 expression in NP cells cultured with different doses of visfatin and treated with visfatin (8 ng/mL) at different time points. (d, h) ELISA analysis of IL-6 expression in the supernatant of NP cells cultured with different doses of visfatin and treated with visfatin (8 ng/mL) at different time points. CTR, control. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 versus control. The data are represented as mean ± standard deviation
Fig 2: Visfatin reduces type II collagen and aggrecan expression and induces the expression of MMP3 in human NP cells. (a-i) Western blot and RT-qPCR analysis of aggrecan, type II collagen, and MMP3 expressions in NP cells cultured with different doses of visfatin. (j, k, l) Western blot and RT-qPCR analysis of type II collagen level in NP cells treated with visfatin (8 ng/mL) at different time points. CTR, control. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001 versus control. The data are represented as mean ± standard deviation
Supplier Page from Abcam for Mouse/Rat Visfatin ELISA Kit