Fig 1: (A), Heatmap showing significantly altered inflammation-related and apoptosis-related genes between WT mice and GSDMD-/- mice on Day 3 after I/R. (B, C), KEGG pathway analysis of inflammatory response-related genes (B) and cardiac function-related genes (C) in WT mice and GSDMD-/- mice after surgery. (D) The results of GO analysis between WT mice and GSDMD-/- mice after I/R.
Fig 2: Gsdmd deficiency inhibited pyroptosis level in LPS-induced ALI and related intestinal barrier impairment. (A) Levels of pyroptosis relative inflammatory cytokines IL-18 and IL-1ß in serum, **P < 0.01, ***P < 0.001, by one-way ANOVA followed by post hoc Newman-Keuls Multiple Comparison Test; (B)The expression of IL-18 and IL-1ß were quantified by immunofluorescence histochemistry and (C) expressions of N-GSDMD, IL-18, IL-1ß and caspase-1 were detected by Wb in lung tissue of WT and Gsdmd-/- groups mice. (D) The expression of N-GSDMD, caspase-1, IL-18 and IL-1ß were detected by Wb in intestinal mucosa tissue of WT and Gsdmd-/- groups mice
Fig 3: (A–C), Serum levels of IL-1ß (A), IL-18 (B) and IL-6 (C) in WT mice and GSDMD-/- mice on Day 3 after I/R injury or sham surgery. (D) Representative images showing immunohistochemical staining of heart tissue from WT mice and GSDMD-/- mice on Day 3 after I/R injury or sham operation. (* and ** indicated p<0.05 and p<0.01, respectively).
Fig 4: GSDMD on LPS-induced pyroptosis is required in ALI. (A) H&E staining of lung sections from WT and Gsdmd-/- groups mice with or without LPS administration under x200 magnification (scale bar = 100 µm);(B) wet/dry Ratio of the lung, ***P < 0.001, by one-way ANOVA followed by post hoc Newman-Keuls Multiple Comparison Test; (C) Levels of the number of total cells, macrophages, and neutrophils in BALF, ***P < 0.001, by two-way ANOVA followed by post hoc Bonferroni’s Multiple Comparison Test ; (D) Expression of IL-6 and TNF-a in serum, ***P < 0.001, by one-way ANOVA followed by post hoc Newman-Keuls Multiple Comparison Test n = 10 for per group. All data are median values from 3 independent experiments. NS means Not significant
Fig 5: GSDMD was required for ALI-induced intestinal barrier damage. (A) H&E staining images of intestinal mucosa tissues from WT and Gsdmd-/- groups mice with or without LPS treatment, under x200 magnification (scale bar = 100 µm); (B) The protein levels of ZO-1 and Occludin in intestinal mucosa tissues from indicated mice were determined by immunofluorescence histochemistry under x200 magnification (scale bar = 100 µm); The alveolar-capillary permeability by measuring(C) DAO and (D) D-lactic acid concentration. ***P < 0.001, by one-way ANOVA followed by post hoc Newman-Keuls Multiple Comparison Test
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