Fig 1: Autophagy gene and protein expression analysis. (A-C) Box plots of qRT-PCR analysis showing gene expression (Relative to GAPDH) of BECN1, ATG3 and ATG7 in term and preterm placentas. n = 20 term and 20 preterm. Data are presented as median and IQR with minima and maxima. Each dot represents individual subject. (D) STRING protein-protein interaction analysis (STRING, Version 11.5). (E,F) Ratio of gene expression of BECN1 and BAX (E), and BECN1 and BCL2 (F) in term and preterm placentas. n = 20 term and 20 preterm. Data are presented as median and IQR with minima and maxima (G,H). Box plots showing ELISA assay of p62/SQSTM1 (G) and CAPN1 (H) on term and preterm villous placenta tissue lysates. Total protein load at 300 µg/ml for each sample. n = 20 term and 20 preterm. Data are presented as median and IQR with minima and maxima. (I) Spearman Rank test correlation analysis between p62/SQSTM1 and CAPN1 protein concentrations on the entire cohort (n = 40). J. Spearman Rank test correlation analysis between p62/SQSTM1 protein concentrations and baby birth weights on the entire cohort (n = 40). Dotted lines represent 95% CI bands of the best-fit lines (Solid). ns = not significant, Mann-Whitney U test (A–H).
Supplier Page from Wuhan Fine Biotech Co., Ltd. for Human CAPN1(calpain 1) ELISA Kit