HiScribe T7 ARCA mRNA Kit E2065S from New England Biolabs

Product Specs
ItemHiScribe T7 ARCA mRNA Kit
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE2065S
Quantity20 reactions
ApplicationsFunctional Genomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Hybridization led to a rewired pluripotency network in the allotetraploid Xenopus laeviseLifeOctober 3, 2023Wesley A Phelps, Matthew D Hurton, Taylor N Ayers, Anne E Carlson, Joel C Rosenbaum, Miler T Leeassembly (NEB #E2621) for pou5f3.3 and BamHI/XbaI for sox3. IVT was done using NEB HiScribe T7 ARCA kit (#E2065S) on NotI-linearized plasmid for 2 hr at 37 °C, then treated with 5 U of TURBO DNaseI (Invitrogen #AM2238)

(2) Maintenance of a host-specific minority mutation in the West Nile virus NS3iScienceAugust 18, 2023Haley S. Caldwell, Lili Kuo, Janice D. Pata, Alan P. Dupuis, Jamie J. Arnold, Calvin Yeager, Jessica Stout, Cheri A. Koetzner, Anne F. Payne, Sean M. Bialosuknia, et al.mRNA transfection reagent,Polyplus,Cat# 101000005 HiScribe® T7 ARCA mRNA Kit,New England Biolabs,Cat# E2065S ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, Culex

(3) The PRMT5/WDR77 complex restricts hepatitis E virus replicationPLOS PathogensJune 5, 2023Xiaohui Ju, Yanying Yu, Wenlin Ren, Lin Dong, Xianbin Meng, Haiteng Deng, Yuchen Nan, Qiang Dingplasmid templates were transcribed in vitro with HiScribe T7 antireverse cap analog (ARCA) mRNA kit (E2065S, NEB) according to the manufacturer’s instructions. Viral RNA was transfected into HepG2C3A cells or

(4) Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrasesNature biotechnologyApril 1, 2023Yarnall MTN, Ioannidi EI, Schmitt-Ulms C, Krajeski RN, Lim J, Villiger L, Zhou W, Jiang K, Garushyants SK, Roberts N, et al.Bxb1 mRNA (NLS-Bxb1) were transcribed and poly-A tailed using the HiScribe™ T7 ARCA mRNA Kit (NEB, E2065S) with 50% supplement of 5-Methyl-CTP and Pseudo-UTP (Jena Biosciences), following the manufacturer’s

(5) eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translationNucleic acids researchFebruary 28, 2023Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, et al.UK), GFP (ab13970, Abcam UK), vinculin (ab129002, Abcam UK). Kits: HiScribe™ T7 ARCA mRNA Kit (NEB E2065S), HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (NEB E2080S), Rabbit Reticulocyte Lysate (Promega

(6) Cytokinesis During the First Division of a Mouse EmbryoFrontiers in Cell and Developmental BiologyFebruary 1, 2022Xiao-Yan Fan, Xing-Hua Wang, Feng-Yun Xie, Jun-Yu Ma, Xiang-Hong Ou, Shi-Ming Luoobtained the mRNA by utilizing an RNA transcription kit (HiScribeTM T7 ARCA mRNA Kit (with tailing), E2065S, New England Biolabs, United States). The GFP-labeled actin and RFP-labeled lysosome plasmids were gifts

(7) Volumetric morphometry reveals spindle width as the best predictor of mammalian spindle scalingJ Cell BiolJanuary 3, 2022Tobias Kletter, Sebastian Reusch, Tommaso Cavazza, Nils Dempewolf, Christian Tischer, Simone Reber2019), and H2B-mScarlet (So et al., 2019). All mRNAs were synthesized using HiScribe T7 ARCA mRNA Kit (E2065S; New England Biolabs) following the manufacturer’s protocol and quantified using the Qubit RNA HS Assay Kit

(8) Noncanonical protease-activated receptor 1 regulates lymphatic differentiation in zebrafishiScienceNovember 19, 2021Lei D, Zhang X, Rouf MA, Mahendra Y, Wen L, Li Y, Zhang X, Li L, Wang L, Zhang T, et al.al., 2007)",N/A LR Clonase II Plus Enzyme mix,Invitrogen,12538200 HiScribe T7 ARCA mRNA kit,NEB,#E2065S DIG RNA Labeling Mix,Roche,11277073910 TB Green Fast RT-PCR Mix,TAKARA,RR430A ,, Experimental models:

(9) Targeting 3′ and 5′ untranslated regions with antisense oligonucleotides to stabilize frataxin mRNA and increase protein expressionNucleic Acids ResearchNovember 18, 2021Yanjie Li et al.T7 RNA polymerase in the presence of 10 mM EU. A HiScribe™ T7 ARCA mRNA Kit (New England Biolabs, Cat# E2065S) was used for in vitro transcription, and the pCAG-FLPe-GFP plasmid (Addgene #13788) was used as a template.

(10) High-Survival Rate After Microinjection of Mouse Oocytes and Early Embryos With mRNA by Combining a Tip Pipette and Piezoelectric-Assisted MicromanipulatorFrontiers in Cell and Developmental BiologySeptember 3, 2021Lei-Ning Chen et al.GFP mRNA was then obtained with an RNA transcription kit [HiScribeTM T7 ARCA mRNA Kit (with tailing), E2065S, New England Biolabs, United States] and divided as 0.8 μl each tube and stored at −80∘C until use. Microinjection

(11) The RNA helicase DHX36â€“G4R1 modulates C9orf72 GGGGCC hexanucleotide repeatâ€“associated translationJournal of Biological ChemistryAugust 1, 2021Yi-Ju Tseng, Siara N. Sandwith, Katelyn M. Green, Antonio E. Chambers, Amy Krans, Heather M. Raimer, Meredith E. Sharlow, Michael A. Reisinger, Adam E. Richardson, Eric D. Routh, et al.in vitro from these plasmids using HiScribe T7 ARCA mRNA Kit, with polyA tailing (NEB; catalog no. E2065S) following the manufacturer's instructions and recovered using RNA Clean and Concentrator-25 kits (Zymo Research

(12) Parental genome unification is highly error-prone in mammalian embryosCellMay 27, 2021Cavazza T, Takeda Y, Politi AZ, Aushev M, Aldag P, Baker C, Choudhary M, Bucevičius J, Lukinavičius G, Elder K, et al.protein expression and purification All mRNAs were synthesized using HiScribe T7 ARCA mRNA Kit (NEB # E2065S) following the manufacturer’s protocol and quantified using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific

(13) CryoSIM: super-resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultrastructural imagingOpticaJuly 20, 2020Michael A. Phillips et al.as a template for in vitro RNA transcription with HiScribe T7 ARCA mRNA kit (New England Biolabs, #E2065S). Transcribed genomic RNA was transfected into BHK-21 using Lipofectamine 2000 reagent (Invitrogen,

(14) Identification of functional cis-acting RNA elements in the hepatitis E virus genome required for viral replicationPLoS pathogensMay 1, 2020Ju X, Xiang G, Gong M, Yang R, Qin J, Li Y, Nan Y, Yang Y, Zhang QC, Ding Q.accordingly [70]. Briefly, biotinylated RNAs were transcribed in vitro by HiScribe T7 ARCA mRNA Kit (NEB, E2065S) according to the manufacturer’s protocol. Biotin-16-UTPs (Roche) were added as 10% of UTP in the reactions.

(15) System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus InfectionMolecular cellApril 4, 2019Garcia-Moreno M, Noerenberg M, Ni S, Järvelin AI, González-Almela E, Lenz CE, Bach-Pages M, Cox V, Avolio R, Davis T, et al.as a template for in vitro RNA transcription with HiScribe T7 ARCA mRNA kit (New England Biolabs, #E2065S). Transcribed genomic RNA was transfected into BHK-21 using Lipofectamine 2000 reagent (Invitrogen,

(16) Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactionsNature CommunicationsDecember 1, 2018Thomas Philipp Hoernes et al.encoding the 5′-part of the eGFP reporter mRNAs were generated with the HiScribe™ T7 ARCA mRNA kit (NEB, E2065S). Transcripts without a 5′-cap were synthesized employing the HiScribe™ T7 High Yield RNA synthesis kit

(17) Design and assessment of engineered CRISPR–Cpf1 and its use for genome editingNature ProtocolsMay 1, 2018Bin Li, Chunxi Zeng, Yizhou DongHemocytometer (Hausser Scientific, cat. no. 3100) HiScribe T7 ARCA mRNA Kit (New England Biolabs, cat. no. E2065S) Human embryonic kidney 293T cell line (American Type Culture Collection, cat. no. CRL-3216) ! CAUTION

(18) The RNA helicase DHX 9 establishes nucleolar heterochromatin, and this activity is required for embryonic stem cell differentiationEMBO reportsJuly 1, 2017Sergio Leone, Dominik Bär, Coenraad Frederik Slabber, Damian Dalcher, Raffaella Santorosequences. IGS-rRNA used for ESCs transfection was synthesized using the HiScribeTM T7 ARCA Kit (Neb E2065S) according manufacturer’s instructions, in order to increase its stability upon transfection. After treatment

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HiScribe T7 ARCA mRNA Kit

HiScribe T7 ARCA mRNA Kit from New England Biolabs

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HiScribe T7 ARCA mRNA Kit from New England Biolabs

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