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NEBNext Single Cell/Low Input cDNA Synt
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NEBNext Single Cell/Low Input cDNA Synt
NEBNext Single Cell/Low Input cDNA Synt from New England Biolabs
Be the first to write a review!
Citations:
(12)
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NEBNext Single Cell/Low Input cDNA Synt
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Description
NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module - 24 rxns
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
NEBNext Single Cell/Low Input cDNA Synt
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
E6421S
Quantity
24 reactions
Applications
DNA Analysis, NGS
Brand/Trademark
New England Biolabs Inc (NEB)
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (12)
(1)
LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversification
Science Advances
November 3, 2023
Raquel Garza, Diahann A. M. Atacho, Anita Adami, Patricia Gerdes, Meghna Vinod, PingHsun Hsieh, Ofelia Karlsson, Vivien Horvath, Pia A. Johansson, Ninoslav Pandiloski,
et al.
October 2019) using the NEBNext Single Cell/Low Input
cDNA Synthesis
&
Amplification
Module (catalog nos.
E6421S
for 24 reactions and
E6421L
for 96 reactions,
New England Biolabs
), the Iso-Seq Express Oligo Kit (catalog
(2)
Long read sequencing to reveal the full complexity of a plant transcriptome by targeting both standard and long workflows
Plant Methods
October 21, 2023
Othman Al-Dossary, Agnelo Furtado, Ardashir KharabianMasouleh, Bader Alsubaie, Ibrahim Al-Mssallem, Robert J. Henry
of the Iso-Seq library, the NEBNext Single Cell/Low Input
cDNA Synthesis
,
Amplification
Module (
NEB
,
E6421S
), and the SMRTbell Express Template Prep Kit 2.0 (PacBio, 100-938-900) were used based on the standard
(3)
Einkorn genomics sheds light on history of the oldest domesticated wheat
Nature
August 2, 2023
Hanin Ibrahim Ahmed, Matthias Heuberger, Adam Schoen, Dal-Hoe Koo, Jesus Quiroz-Chavez, Laxman Adhikari, John Raupp, Stéphane Cauet, Nathalie Rodde, Charlotte Cravero,
et al.
separately using NEBNext Single Cell/Low Input
cDNA Synthesis
&
Amplification
Module (
New England Biolabs
,
E6421S
). The
ProNex Size-Selective Purification System
(
Promega
,
NG2001
) was used for size selection. One SMRT
(4)
Direct haplotype-resolved 5-base HiFi sequencing for genome-wide profiling of hypermethylation outliers in a rare disease cohort
Nature Communications
May 29, 2023
Warren A. Cheung, Adam F. Johnson, William J. Rowell, Emily Farrow, Richard Hall, Ana S. A. Cohen, John C. Means, Tricia N. Zion, Daniel M. Portik, Christopher T. Saunders,
et al.
cDNA synthesis
with a NEBNext Single Cell/Low Input
cDNA Synthesis
&
Amplification
Module (
NEB
, Cat. No.
E6421S
) and an Iso-Seq Express Oligo Kit (PacBio, Cat. No. 101-737-500) as described in the Iso-Seq Express
(5)
The gyrfalcon (Falcon rusticolus) genome
G3 Genes|Genomes|Genetics
March 9, 2023
Andrea Zuccolo, Sara Mfarrej, Mirko Celii, Saule Mussurova, Luis F Rivera, Victor Llaca, Nahed Mohammed, Arnab Pain, Abdulmajeed Fahad Alrefaei, Abdulwahed Fahad Alrefaei,
et al.
was done using NEBNext Single Cell/Low input
cDNA synthesis
and
amplification
module (
NEB
, Cat No.:
E6421S
) with Iso-Seq Express Oligo Kit (Pacbio, 101-737-500) and Iso-seq library was built with SMRTbell Express
(6)
A chromosome-level reference genome and pangenome for barn swallow population genomics
Cell Reports
January 31, 2023
Simona Secomandi, Guido R. Gallo, Marcella Sozzoni, Alessio Iannucci, Elena Galati, Linelle Abueg, Jennifer Balacco, Manuela Caprioli, William Chow, Claudio Ciofi,
et al.
NEBNext Single Cell/Low Input
cDNA Synthesis
&
Amplification
Module (
New England BioLabs
, cat. no.
E6421S
) and Iso-Seq Express Oligo Kit (PacBio PN 10 1-737-500) from 300 ng of total RNA for both brain and
(7)
NAb-seq: an accurate, rapid, and cost-effective method for antibody long-read sequencing in hybridoma cell lines and single B cells
mAbs
December 31, 2022
Hema Preethi Subas Satish, Kathleen Zeglinski, Rachel T. Uren, Stephen L. Nutt, Matthew E. Ritchie, Quentin Gouil, Ruth M. Kluck
cells were prepared using the NEBNext® single cell/low input
cDNA synthesis
and
amplification
module (
E6421S
;
New England BioLabs
Inc.). As a single mammalian cell contains only 0.1 pg mRNA,42 this module is required
(8)
Single-cell analysis and functional characterization uncover the stem cell hierarchies and developmental origins of rhabdomyosarcoma
Nature Cancer
August 18, 2022
Yun Wei, Qian Qin, Chuan Yan, Madeline N. Hayes, Sara P. Garcia, Haibin Xi, Daniel Do, Alexander H. Jin, Tiffany C. Eng, Karin M. McCarthy,
et al.
the NEBNext Single Cell/Low Input
cDNA Synthesis
and
Amplification
Module kit (
New England Biolabs
#
E6421S
). Amplified cDNA was then used in each
quantitative PCR
(
PowerUp SYBR Green Master Mix
,
Fisher Scientific
(9)
Long-read genome sequencing of bread wheat facilitates disease resistance gene cloning
Nature Genetics
March 14, 2022
Naveenkumar Athiyannan, Michael Abrouk, Willem H. P. Boshoff, Stéphane Cauet, Nathalie Rodde, David Kudrna, Nahed Mohammed, Jan Bettgenhaeuser, Kirsty S. Botha, Shannon S. Derman,
et al.
using the NEBNext Single Cell/Low Input
cDNA Synthesis
&
Amplification
Module (
New England Biolabs
,
E6421S
) and the
ProNex Size-Selective Purification System
(
Promega
,
NG2001
) for size selection. In brief, 300
(10)
Single worm transcriptomics identifies a developmental core network of oscillating genes with deep conservation across nematodes
Genome Research
September 1, 2021
Shuai Sun, Christian Rödelsperger, Ralf J. Sommer
liquid nitrogen. Afterward, 3 µL of lysis buffer (low input
cDNA synthesis
&
amplification
module;
NEB
E6421S
) was added, and samples were incubated for 40 min at 65°C, followed by 1 min at 85°C. To remove genomic
(11)
Identification of neural progenitor cells and their progeny reveals long distance migration in the developing octopus brain
eLife
August 24, 2021
Deryckere A, Styfhals R, Elagoz AM, Maes GE, Seuntjens E.
kit,NEBNext Single Cell/Low Input
cDNA Synthesis
and
Amplification
Module,
New England BioLabs
.,Cat#:
E6421S
, Commercial
assay
or kit,
Superscript III Reverse Transcriptase
,
Invitrogen
,Cat#: 18080–044, Commercial
(12)
Reference genome and demographic history of the most endangered marine mammal, the vaquita
Molecular Ecology Resources
May 1, 2021
Phillip A. Morin et al.
reverse transcribed using the NEBNext Single Cell/Low Input
cDNA Synthesis
&
Amplification
Module (
NEB
E6421S
) from 238 ng total RNA. Amplified cDNA was cleaned with 86 µl ProNex beads. The PacBio Iso‐seq library
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NEBNext Single Cell/Low Input cDNA Synt from New England Biolabs
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