Fig 1: Cell trajectory analysis shows the evolution of mLECs. A) Labeling mLECs with real grouping information based on pseudotime trajectory map. B) Pseudotime trajectory map. C) Trajectory of representative genes in mLECs. D) Heatmap showing the top 30 pseudotime-related genes. E) Representative two pseudo-time-related genes (S100a6 and Cldn5) based on q values. F,G) Increased expression of THBS1 and S100A6 are associated with poor prognosis, n = 48, *p < 0.05 by paired two-tailed Student's t-test. H) Linear relationship between THBS1 and S100A6 expression, r = 0.3779, p = 0.0081 by Spearman's rank test. H) Representative confocal images of mLVs region of sham and SAH 24 h group. Enlarged view of selected region in the merged photo (yellow dotted box) are listed on the right in each group. Lyve1 (blue), S100a6 (green), and CD31 (red). Yellow arrows indicate where mLVs lie. Scale bar: 800 µm for the holistic view and 100 µm for the enlarged view.
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