Fig 1: The concentrations of sPD-1 and sPD-L1 in plasma and the concentrations of PD-1 and PD-L1 in kidney homogenate (A). Correlation was shown between sPD-1 in plasma and PD-1 in kidney homogenate (r = 0.774, p<0.001) (B), between sPD-L1 in plasma and PD-L1 in kidney homogenate (r = 0.881 p<0.001) (B) using Spearman’s test.
Fig 2: Preoperative serum levels between healthy volunteers and RCC patients. Preoperative serum levels of VEGF (A), B7-H4 (B) and PD-L1 (C) were higher in RCC patients than those in healthy volunteers, whereas those of PD-1 (D) were not. The median value is the central line, the box is the interquartile range, the bars are the full range, and the points are the outliers. Bold circled P values were obtained by comparing the two groups with the Mann–Whitney U-test.
Fig 3: The expression of PD-1 in kidney tissue of mice. The IHC of PD-1 was shown in control groups (A), sham groups (B) and IRI groups (C). Quantification is represented in panel (D). Data are expressed as mean ± standard deviation. Difference between IRI group and sham group was made using unpaired Student’s t-test for normally distributed data. Images, × 200; original scale bar, 100 µm. **p<0.01. ***p<0.001.
Fig 4: Relationship between serum B7 family molecules. Spearman rank correlation between the preoperative serum levels of VEGF and B7-H4/PD-L1 in all 171 cases (A and C) and in 83 metastatic (M1) cases at nephrectomy (B and D). The preoperative serum levels of B7-H4 and PD-1 were positively associated with those of VEGF in all case and in M1 cases.
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