Fig 1: Effect of obesity and weigh loss achieved by sleeve gastrectomy on the expression of the guanylin system in rat pancreas. (A) Cellular localization of the receptor of guanylin peptides, GUCY2C, in the islets of Langerhans (left panel, magnification 200X) as well as in inter- and intralobular pancreatic ducts (right panel, magnification 400X) in rat pancreas. Bar graphs illustrate the impact of weight gain induced by HFD feeding (B, C) and weight loss achieved by sleeve gastrectomy (D, E) on pancreatic mRNA and protein of GUCA2A, GUCA2B and their receptor GUCY2C. The gene and protein expression in lean control rats fed a ND or in the sham-operated group was assumed to be 1. Representative blots are shown. Statistical differences were analyzed using Student’s t-test or one-way ANOVA followed by a Tukey’s post hoc test, where appropriate. *P<0.05 vs lean or sham-operated rats.
Fig 2: Increase in plasma guanylin peptides and GLP-1 levels following sleeve gastrectomy in experimental diet-induced obesity. Fasting GUCA2A (A), GUCA2B (B) and GLP-1 (C) concentrations one month after surgical or dietary interventions. Statistical differences were analyzed using a one-way ANOVA followed by a Tukey’s post hoc test. *P<0.05 vs sham-operated rats.
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