Fig 1: MIF ablation inhibits oxidative stress in hepatic I/R injury. (A) ELISA detection of MDA, SOD and GSH level in serum of WT and MIF-KO mice after hepatic I/R surgery (n = 6 per group). (B) DHE staining in liver sections from WT and MIF-KO mice after hepatic I/R surgery (n = 4/group). Scale bar, 50 µm. (C) ELISA detection of MDA, SOD and GSH level in serum of AAV-GFP or AAV-MIF mice after hepatic I/R surgery (n = 6 per group). (D) DHE staining in liver sections from AAV-GFP or AAV-MIF mice after hepatic I/R surgery (n = 4/group). Scale bar, 50 µm. All data are shown as the mean ± SD. Levels of statistical significance are indicated as **p < 0.01. For statistical analysis, one-way ANOVA with Bonferroni’s post hoc analysis or Tamhane’s T2 post hoc analysis and two-tailed Student t test were used.
Fig 2: CF improved renal function, oxidative stress and inflammation levels in SAMP8 mice. a-d Effect of CF on the urine volume, urine protein levels, and serum Cr and urea nitrogen levels, respectively. e-h The level of SOD, GSH-Px, TNF-a and IL-1ß in serum, respectively. Con: SAMR1 mice; M: SAMP8 mice; CF-L: Treated with low-dose CF ethanol extract; CF-M: Treated with medium-dose CF ethanol extract; CF-H: Treated with high-dose CF ethanol extract; Values expressed as mean ± SD; *P < 0.05 and **P < 0.01 vs. M group (n = 5–12)
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