Fig 1: miR-155 increases the pro-inflammatory cytokines expression in BV-2 microglial cells. BV-2 microglial cells were transfected with mimic control, miR-155 mimics or treated with LPS. (A) ELISA and (B) reverse transcription-quantitative polymerase chain reaction assays were performed to measure the expression levels of IL-6, IL-10, TNF-a, TNF-ß and IDO1 in BV-2 microglial cells. #P<0.05 vs. control; **P<0.01, ***P<0.001 vs. mimic control. miR, microRNA; LPS, lipopolysaccharide; IL, interleukin; TNF, tumor necrosis factor; IDO1, indoleamine 2,3-dioxygenase 1.
Fig 2: miR-155 upregulates TNF-a, TNF-ß and IDO1 expression in BV-2 microglial cells. BV-2 microglial cells were transfected with mimic control, miR-155 mimics or treated with LPS. Western blotting was performed to evaluate the expression levels of TNF-a, TNF-ß and IDO1 in BV-2 microglial cells. #P<0.05 vs. control; **P<0.01, ***P<0.001 vs. mimic control. miR, microRNA; LPS, lipopolysaccharide; TNF, tumor necrosis factor; IDO1, indoleamine 2,3-dioxygenase 1.
Supplier Page from CUSABIO Technology LLC for Mouse Indoleamine 2,3-dioxygenase 1(IDO1) ELISA Kit