Fig 1: Post-operative serum SIRPA concentration from 54 patients using Cusabio SIRPA ELISA kit. Patients were grouped into no ventriculotomy (n = 28), myectomy (n = 14), ventriculotomy (n = 7) and non-cardiac surgery i.e. liver and renal transplants as negative controls (n = 6). Post-operative serum SIRPA concentrations were significantly higher in patients receiving ventriculotomy compared to other groups (*p < 0.0001 vs no ventriculotomy, §p = 0.0004 vs myectomy and †p = 0.0001 vs non-cardiac)
Fig 2: Cusabio and Elabscience SIRPA ELISA kits failed to recognize recombinant human SIRPA protein. a Cusabio SIRPA ELISA kit (CSB-EL021334HU) recognized its own calibrator diluted in buffer from Cusabio ELISA kit generating a linear curve (diamonds) but did not recognize recombinant human SIRPA protein (squares); b Elabscience SIRPA ELISA kit (E-EL-H1573) recognized its own calibrator diluted in buffer from Elabscience ELISA kit generating a linear curve (diamonds) but did not recognize recombinant human SIRPA protein (squares). X- axis represents known standard concentration (ng/ml) and Y-axis represents optical density (OD) measured at 450 nm. c Western blot of rhSIRPA using SIRPA antibody (Biolegend, catalog # 323805) detected a ~55 kda band in rhSIRPA but not in calibrators from Cusabio and Elabscience SIRPA ELISA kits
Supplier Page from CUSABIO Technology LLC for Human Tyrosine-protein phosphatase non-receptor type substrate 1(SIRPA) ELISA Kit