Fig 1: Gene expression and protein release of ScAT and IFP and cytokine concentration in synovial fluid. mRNA was quantified by real time RT PCR (RT qPCR). Values for MMP-1, MMP-3, MMP-13 and TIMP-2 were normalized to the housekeeping gene (β-actin) and presented as the fold increase compared to control ScAT on the left y-axis. Protein concentration of MMP-1, MMP-3 and MMP-13 secreted by ScAT and IFP as well as in synovial fluid were measured by ELISA and presented as ng/ ml on the right y-axis. * denotes statistically significant differences with p < 0.05 and ** p < 0.01. White boxes demonstrate control group and grey boxes samples from dogs with CCLD
Fig 2: The results of cytokines analysis in a canine model of OA. Each cytokine analysis of (A) TNF-α, (B) IL-1β, and (C) MMP-3 was carried out on synovial fluid obtained during post-mortem examination, utilizing specific ELISA kits for each cytokine. Data are expressed as mean ± S.D. Day of first test article administration was designated day 0. G1: normal control, G2: vehicle control, G3: positive control, G4: 200 mg IR, G5: 300 mg IR, G6: 300 mg MR, G7: CR-1. ***/**/* A significant difference at p < 0.001/p < 0.01/p < 0.05 level compared to the G1. ###/##/# A significant difference at p < 0.001/p < 0.01/p < 0.05 level compared to the G2. $$ A significant difference at p < 0.01 level compared to the G3. ^^ A significant difference at p < 0.01 level compared to the G4. % A significant difference at p < 0.05 level compared to the G5. &&&/&&/& A significant difference at p < 0.001/p < 0.01/p < 0.05 level compared to the G6.
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