Fig 1: Overexpression of AOC3 regulates iNOS expression and its activity in RIMECs under hypoxia. (a) SSAO and iNOS activity in RIMECs and RIMSCs co-culture medium were determined using Fluoro SSAO™ Fluorescent SSAO Detection kit and PathScan® Total iNOS Sandwich ELISA Kit. (b) SSAO and iNOS protein expression in RIMECs, as determined by the western blotting. GAPDH was used as an internal control (*P < 0.05, **P < 0.01, and ***P < 0.001).
Fig 2: SSAO can regulate LPS-induced iNOS expression in RIMECs. (a) SSAO and iNOS activity in RIMECs culture medium were determined using Fluoro SSAO™ Fluorescent SSAO Detection kit and PathScan® Total iNOS Sandwich ELISA Kit. (b) SSAO and iNOS protein expression in RIMECs, as determined by the western blotting. GAPDH was used as an internal control (*P < 0.05, **P < 0.01, and ***P < 0.001).
Fig 3: Overexpression of AOC3 increases the ROCK1 expression and phosphorylation of MLC20 in RIMSCs under hypoxia. ROCK1 and p-MLC20 protein expression in RIMSCs were determined by western blotting. GAPDH and MLC20 were used as internal controls, respectively (*P < 0.05, **P < 0.01, and ***P < 0.001).
Fig 4: Construction of adenovirus vector for AOC3 overexpression. (a) Adenoviral vector (pAD/CMV/V5-DEST) for AOC3 overexpression. (b) Light and fluorescence microscopic images confirmed the successful transfection of RIMECs with pAd-AOC3-IRES-EGFP (magnification, 200×).
Fig 5: Detection of AOC3 overexpression in RIMECs by adenovirus. (a) AOC3 mRNA expression in RIMECs as determined by qRT-PCR. GAPDH was used as an internal control. (b) SSAO protein expression in RIMECs was determined by Western-Blot. GAPDH was used as an internal control (*P < 0.05, **P < 0.01, and ***P < 0.001).
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Rat VAP-1/AOC3 ELISA Kit (Colorimetric)