Fig 1: Silencing of ALOX5 alleviates inflammatory response and fibrosis in HG-induced SV40 MES-13 cells. (A-C) The HG-induced SV40 MES-13 cells were transfected with si-ALOX5 or si-control, and the inflammatory cytokines, including TNF-α, IL-6 and IL-8 were evaluated using their corresponding ELISA kits. (D) The expression level of fibrosis-related proteins was assessed using western blotting. ***P<0.001 vs. NG; ##P<0.01 and ###P<0.001 vs. HG + si-control. ALOX5, 5-lipoxygenase; HG, high glucose; si-, siRNA; ELISA, enzyme-linked immunosorbent; NG, normal glucose; COL4, collagen type IV; FN, fibronectin; TGF-β1, transforming growth factor β1.