Mouse TNF-alpha ELISA Kit RK00027 from ABclonal Technology

Product Specs
ItemMouse TNF-alpha ELISA Kit
CompanyABclonal Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberRK00027
Quantity96 T
ApplicationsELISA
Detection Range15.6-1000 pg/ml
Sample TypeSerum, plasma and cell culture supernates
Assay Time4 hours
ComponentsMicrowell Plate coated with monoclonal antibody to Mouse TNF-alpha
Biotin-Conjugate anti-Mouse TNF-alpha antibody
Streptavidin-HRP
Mouse TNF-alpha Standard lyophilized
Sample Diluent
Biotin-Conjugate antibody Diluent
Streptavidin-HRP Diluent
Wash Buffer Concentrate 20x
Substrate Solution (tetramethyl-benzidine)
Stop Solution
Plate Sealers
Specification
Sensitivity6.5 pg/mL
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesDI, DIF, TNF-, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tn, Tnfa, Tnfs, Tnfsf1a, Tnlg1f

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ABclonal Technology

500W Cummings Park, Ste. 6500

Woburn, MA 01801

United States

Phone: 888.754.5670
Int’l: +1857.259.4898

Email: service@abclonal.com

Company Profile

Website: https://abclonal.com/

(1) The effect and mechanism of Huangqin-Baishao herb pair in the treatment of dextran sulfate sodium-induced ulcerative colitisHeliyonNovember 30, 2023Bailu Duan, Qiong Hu, Fengmin Ding, Fang Huang, Wei Wang, Nina Yin, Zhe Liu, Song Zhang, Dongchu He, Qiping Lu(IL-6) in colons were quantified measured by using mouse enzyme-linked immunosorbent assay (ELISA) Kits (RK00027, RK00006 and RK00008, ABclonal, Wuhan, China), respectively, strictly according to the manufacturer's

(2) Aerobic exercise combined with chlorogenic acid exerts neuroprotective effects and reverses cognitive decline in Alzheimer’s disease model mice (APP/PS1) via the SIRT1/ /PGC-1α/PPARγ signaling pathwayFrontiers in Aging NeuroscienceNovember 16, 2023Dan Shi, Zikang Hao, Wenxiao Qi, Fengyi Jiang, Kerui Liu, Xiao Shihippocampal tissue homogenates according to the ELISA kit manufacturer’s instructions (RK00006; RK00008; RK00027, ABclonal Biotechnology Co., Ltd., Wuhan, China) and the OD values of each group of samples were measured

(3) Liraglutide Attenuates Aortic Valve Calcification in a High-Cholesterol-Diet-Induced Experimental Calcific Aortic Valve Disease Model in Apolipoprotein E-Deficient MiceJournal of Cardiovascular Development and DiseaseSeptember 6, 2023Yangzhao Zhou, Zhaoshun Yuan, Min Wang, Zhiyuan Zhang, Changming Tan, Jiaolian Yu, Yanfeng Bi, Xiaobo Liao, Xinmin Zhou, Md Sayed Ali Sheikh, et al.Analysis Plasma insulin (E-EL-M1382c, Elabscience, Wuhan, China), GLP-1 (E-EL-M3012, Elabscience), TNF-α (RK00027, ABclonal, Wuhan, China), IL-6 (RK00008, ABclonal), and IL-4 (RK00036, ABclonal) levels were determined

(4) Male‐Biased Gut Microbiome and Metabolites Aggravate Colorectal Cancer DevelopmentAdvanced ScienceSeptember 1, 2023Ling Wang, Yi‐Xuan Tu, Lu Chen, Yuan Zhang, Xue‐Ling Pan, Shu‐Qiao Yang, Shuai‐Jie Zhang, Sheng‐Hui Li, Ke‐Chun Yu, Shuo Song, et al.level were measured with an ELISA kit (LPS: catalog number RK04263, ABclonal; TNF‐α: catalog number RK00027, ABclonal; IL‐10: catalog number RK00016, ABclonal). All testing procedures were performed according

(5) Isoalantolactone relieves depression-like behaviors in mice after chronic social defeat stress via the gut-brain axisPsychopharmacology (Berl)August 1, 2023Siming Wang, Qihan Cai, Lu Xu, Yanan Sun, Mengmeng Wang, Yu Wang, Lili Zhang, Keqing Li, Zhiyu Nimeasurement Precoated ELIZA kits were used to detect plasma IL-6 (RK00008; ABclonal, USA) and TNF-α (RK00027; ABclonal, USA) according to the manufacturer’s instructions. Western blot analysis PFC tissues were

(6) RIPK3-MLKL necroptotic signalling amplifies STING pathway and exacerbates lethal sepsisClinical and translational medicineJuly 1, 2023Zhang X, Wu J, Liu Q, Li X, Yang Y, Wu L, Wu X, Zhao Y, Ren J.protein levels of murine IFN‐β (RK00420; ABclonal), murine IL‐6 (KE10007; Proteintech) and murine TNF‐α (RK00027; ABclonal) in the plasma were tested via commercially ELISA kits. 4.9 Histology, immunohistochemistry

(7) Caffeic acid, but not ferulic acid, inhibits macrophage pyroptosis by directly blocking gasdermin D activationMedComm (2020)June 1, 2023Mingjiang Liu, Dandan Liu, Chenglong Yu, Hua hao Fan, Xin Zhao, Huiwen Wang, Chi Zhang, Minxia Zhang, Ruonan Bo, Shasha He, et al.(C2006) were bought from Beyotime (Shanghai, China). ELISA kits for murine IL‐1β (RK00006) and TNF‐α (RK00027) were bought from ABclonal (Wuhan, China). BODIPY 581⁄591 C11 (D3861) was bought from Invitrogen (Carlsbad,

(8) Mevalonate improves anti-PD-1/PD-L1 efficacy by stabilizing CD274 mRNAActa pharmaceutica Sinica. BJune 1, 2023Zhang W, Pan X, Xu Y, Guo H, Zheng M, Chen X, Wu H, Luan F, He Q, Ding L, et al.the mouse IFN-γ (RK00019, ABclonal, China), mouse IL-2 (RK00007, ABclonal, China) and mouse TNF-α (RK00027, ABclonal, China) ELISA Kit according to the manufacturer's instructions. 2.17 Animal experiments Animal

(9) Monomethyl fumarate prevents alloimmune rejection in mouse heart transplantation by inducing tolerogenic dendritic cellsActa Biochimica et Biophysica SinicaMay 16, 2023Juntao Chen, Yirui Cao, Ouyang Jia, Xuanchuan Wang, Yongsheng Luo, Yin Celeste Cheuk, Tongyu Zhu, Dong Zhu, Yi Zhang, Jina WangThe mouse ELISA kits used were as follows: IL-6 (RK00008), IL-1β (RK00006), IFN-γ (RK00019), TNF-α (RK00027), IL-10 (RK00016), and TGF-β1 (RK00057). Cervical cardiac transplantation model Allogeneic heterotopic

(10) Xuanfei Baidu Decoction regulates NETs formation via CXCL2/CXCR2 signaling pathway that is involved in acute lung injuryBiomedicine & PharmacotherapyMay 1, 2023Mengen Zhou, Yiman Liu, Honglin Qin, Ting Shang, Zhifeng Xue, Shuang Yang, Han Zhang, Jian Yangwere purchased from Bioss (Beijing, China). Mouse IL-6 ELISA kit (RK00008) and mouse TNF-α ELISA kit (RK00027) were purchased from ABclonal (Wuhan, China). Percoll and Dnase I (D8070–15KU) were obtained from Solarbio

(11) Xuanfei Baidu Decoction suppresses complement overactivation and ameliorates IgG immune complex-induced acute lung injury by inhibiting JAK2/STAT3/SOCS3 and NF-κB signaling pathwayPhytomedicine : international journal of phytotherapy and phytopharmacologyJanuary 1, 2023Li C, Li Y, Zhang H, Zhuo Y, Zhang L, Yang L, Gao Q, Tu Z, Shao R, Wang Y, et al.IL-1β and TNFα and MCP-1 in lung tissues were estimated by using the ELISA kits (RK00008, RK00006, RK00027, RK00381; ABclonal, Wuhan, China) according to the manufacturer's protocols. Preparation of XFBD-containing

(12) Abrocitinib Attenuates Microglia-Mediated Neuroinflammation after Traumatic Brain Injury via Inhibiting the JAK1/STAT1/NF-κB PathwayCellsNovember 13, 2022Li T, Li L, Peng R, Hao H, Zhang H, Gao Y, Wang C, Li F, Liu X, Chen F, et al.Inflammatory factors were detected in the brain samples using ELISA kits for TNF-α, IL-1β, IL-6, and IL-18 (RK00027, RK00006, RK00008, RK00104, Abclonal, Wuhan, China). The measured OD values were converted into concentration

(13) Identification and exploration of pharmacological pyroptosis-related biomarkers of ulcerative colitisFrontiers in ImmunologyOctober 13, 2022Kaiwei Chen, Shipeng Shang, Shengnan Yu, Luwen Cui, Shangyong Li, Ningning He(3,500 rpm, 4°C, 30 min) after picking out the eyeball. The ELISA kit was purchased from ABclonal (#RK00027, Wuhan, China). The serum levels were measured based on the instruction of the ELISA kit. Statistical

(14) Zika virus causes placental pyroptosis and associated adverse fetal outcomes by activating GSDMEeLifeAugust 16, 2022Zhao Z, Li Q, Ashraf U, Yang M, Zhu W, Gu J, Chen Z, Gu C, Si Y, Cao S, et al.experiments. The concentration of TNF-α was determined by mouse TNF-α ELISA kit (Abclonal Technology, RK00027) according to the manufacturer’s instructions. Statistical analysis All data were analyzed with the

(15) Cangma Huadu granules attenuate H1N1 virus-induced severe lung injury correlated with repressed apoptosis and altered gut microbiomeFrontiers in MicrobiologyAugust 8, 2022Mingjiang Liu et al.cytokines and oxidative stress measurement Mouse ELISA kits for IL-1β (RK00006), IL-10 (RK00006), TNF-α (RK00027), and IFN-γ (RK00019) were all purchased from ABclonal Biotechnology Co., Ltd., China. The oxidative

(16) Extract of Corallodiscus flabellata attenuates renal fibrosis in SAMP8 mice via the Wnt/β-catenin/RAS signaling pathwayBMC complementary medicine and therapiesFebruary 28, 2022Cao B, Zeng M, Si Y, Zhang B, Wang Y, Xu R, Huang Y, Feng W, Zheng X.Nanjing, China), interleukin-1β (IL-1β, RK00006, ABclonal, Wuhan, China), tumor necrosis factor-α (TNF-α, RK00027, ABclonal, Wuhan, China), urea nitrogen (C013-2-1, Nanjing Jiancheng, Nanjing, China), creatinine (Cr,

(17) Forsythoside A Mitigates Alzheimer's-like Pathology by Inhibiting Ferroptosis-mediated Neuroinflammation via Nrf2/GPX4 Axis ActivationInternational Journal of Biological SciencesFebruary 28, 2022Chunyue Wang, Shanshan Chen, Hangyu Guo, Hongbo Jiang, Honghan Liu, Haoran Fu, Di Wang(RK00057), monocyte chemoattractant protein-1 (MCP-1) (RK00381), IL-1β (RK00006), IL-6 (RK00008), and TNF-α (RK00027) in brains were analyzed using a commercial kit (ABclonal, Wuhan, China) according to the manufacturer's

(18) Folic Acid Attenuates High-Fat Diet-Induced Osteoporosis Through the AMPK Signaling PathwayFrontiers in Cell and Developmental BiologyJanuary 3, 2022Haiting He, Yaxi Zhang, Yue Sun, Yanwei Zhang, Jingjing Xu, Yuzhen Yang, Jihua ChenBioengineering Institute (Nanjing, China). The ELISA kits for detecting tumor necrosis factor-α (TNF-α, RK00027), interleukin-6 (IL-6, RK00008), β-actin (AC026), acetyl-CoA carboxylase (ACC, A15606) and carnitine

(19) The ER stress sensor inositol-requiring enzyme 1α in Kupffer cells promotes hepatic ischemia-reperfusion injuryJournal of Biological ChemistryJanuary 1, 2022Jie Cai et al.cells were quantified using Image J software. Serum measurements Serum levels of TNFα (ABclonal, #RK00027), IL-1β (ABclonal, #RK00006), CCL-2 (ABclonal, #RK00381), and CXCL-10 (ABclonal, #RK00054) were measured

(20) Theabrownin and Poria cocos Polysaccharide Improve Lipid Metabolism via Modulation of Bile Acid and Fatty Acid MetabolismFrontiers in pharmacologyJanuary 1, 2022Wang J, Zheng D, Huang F, Zhao A, Kuang J, Ren Z, Chen T, Lei J, Lin J, Wang X, et al.(ELISA) The levels of TNF-α, IL-6, and IL-10 in serum and liver tissue were evaluated with an ELISA kit (RK00027, RK00008 and RK00016, respectively, ABclonal, Wuhan, China) according to the manufacturer’s instructions. Statistical

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Mouse TNF-alpha ELISA Kit from ABclonal Technology

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Mouse TNF-alpha ELISA Kit from ABclonal Technology

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