Citrullinated Histone H3 (Clone 11D3) ELISA Kit 501620 from Cayman Chemical

Product Specs
ItemCitrullinated Histone H3 (Clone 11D3) ELISA Kit
CompanyCayman Chemical
Price Pricing Info Supplier Page View Company Product Page
Catalog Number501620
Quantity96 Well
ApplicationsELISA
Detection Range0.15-10 ng/ml
Clone11D3
Research AreaImmunology & Inflammation, Innate Immunity, NETosis

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Evaluation of selected parameters of inflammation, coagulation system, and formation of extracellular neutrophil traps (NETs) in the perioperative period in patients undergoing endovascular treatment of thoracoabdominal aneurysm with a branched device (t-Branch)Frontiers in Cardiovascular MedicineSeptember 6, 2023Milena Michalska, Tadeusz Grochowiecki, Aleksandra Wyczałkowska-Tomasik, Leszek Pączek, Tomasz Jakimowicz, Andrzej Cacko, Katarzyna Jama, Albert Stec, Ewa Sikorska, Sławomir Nazarewski, et al.enzyme immunoassay using the ELISA method. Cayman's Citrullinated Histone H3 (Clone 11D3) (Cat. No. 501620, Cayman) ELISA kit was used in the investigation. Serum served as the research's initial stage. The

(2) Quantitative cytofluorimetric analysis of mouse neutrophil extracellular trapsSTAR ProtocolsSeptember 1, 2023Valentina Poli, Marco Di Gioia, Ivan Zanoniformation via ELISA kits. We successfully measured the quantity of Citrullinated histone H3 (Cayman, cat#501620) and quantity of myeloperoxidase (MPO)-DNA complexes by using a modified Cell Death Detection ELISA

(3) Low-density granulocytes display immature cells with enhanced NET formation in people living with HIVSci RepAugust 16, 2023Juwon Park, Logan S. Dean, Jack Heckl, Louie Mar Gangcuangco, Te-Kie Pedro, Michelle D. Tallquist, Todd B. Seto, Bruce Shiramizu, Dominic C. Chow, Cecilia M. Shikumaplasma using the enzyme-linked immunosorbent assay according to the manufacturer’s instructions (Cayman, 501620). We used previously generated plasma cytokine data to assess associations of circulating NET markers

(4) Chronic inflammation, neutrophil activity, and autoreactivity splits long COVIDNature communicationsJuly 14, 2023Woodruff MC, Bonham KS, Anam FA, Walker TA, Faliti CE, Ishii Y, Kaminski CY, Ruunstrom MC, Cooper KR, Truong AD, et al.biomarkers Cit-H3 levels in the plasma were quantified using the Citrullinated Histone H3 ELISA Kit (Cayman, 501620) according to the manufacturer’s instructions. Patient plasma was diluted 1:10 prior to assay loading.

(5) Elevated cfDNA after exercise is derived primarily from mature polymorphonuclear neutrophils, with a minor contribution of cardiomyocytesCell Reports MedicineJune 20, 2023Ori Fridlich, Ayelet Peretz, Ilana Fox-Fisher, Sheina Pyanzin, Ziv Dadon, Eilon Shcolnik, Ronen Sadeh, Gavriel Fialkoff, Israa Sharkia, Joshua Moss, et al.1:2 in PBS, and citrullinated histone H3 was measured with the Citrullinated Histone H3 ELISA kit (501620, Cayman Chemical, Ann Arbor, MI) according to manufacturer’s protocol. Immunostaining for NETs Three

(6) Prevalence of Antiphospholipid Antibodies and Association With Incident Cardiovascular EventsJAMA Network OpenApril 4, 2023Yu Zuo, Sherwin Navaz, Wenying Liang, Chun Li, Colby R. Ayers, Christine E. Rysenga, Alyssa Harbaugh, Gary L. Norman, E. Blair Solow, Bonnie Bermas, et al.H3 was quantified using the Citrullinated Histone H3 Enzyme-Linked Immunoassay (ELISA) Kit (Cayman, 501620) according to the manufacturer’s instructions. In-Cell ELISA Human coronary artery endothelial cell

(7) Neutrophil extracellular traps as a unique target in the treatment of chemotherapy-induced peripheral neuropathyeBioMedicineApril 1, 2023Chao-Yu Wang, Tong-Tong Lin, Liang Hu, Chen-Jie Xu, Fan Hu, Li Wan, Xing Yang, Xue-Feng Wu, Xiao-Tao Zhang, Yan Li, et al.quantified according to the manufacturer's instructions with the Citrullinated Histone H3 ELISA Kit (501620, Cayman), NETosis Assay Kit (601010, Cayman) and Quant-iT PicoGreen dsDNA Assay kit (P11496, Invitrogen).

(8) Alleviation of arthritis through prevention of neutrophil extracellular traps by an orally available inhibitor of protein arginine deiminase 4Scientific ReportsFebruary 23, 2023Chandru Gajendran, Shoichi Fukui, Naveen M. Sadhu, Mohammed Zainuddin, Sridharan Rajagopal, Ramachandraiah Gosu, Sarah Gutch, Saeko Fukui, Casey E. Sheehy, Long Chu, et al.Enzyme linked immunosorbent Assay (ELISA) was performed as per manufacturer’s instructions (Cayman chemicals, Catalog No: 501620). Neutrophils obtained as mentioned above were counted and cell suspension was prepared in complete

(9) Alpha-1 antitrypsin limits neutrophil extracellular trap disruption of airway epithelial barrier functionFrontiers in ImmunologyJanuary 10, 2023K. M. Hudock, M. S. Collins, M. A. Imbrogno, E. L. Kramer, J. J. Brewington, A. Ziady, N. Zhang, J. Snowball, Y. Xu, B. C. Carey, et al.integrity. These included 5µg/ml human gDNA (Promega), 500ng/ml citrullinated histones (Cayman Chemical #501620), 100mU/ml human NE protein (Athens Research & Technology #16-14-051200, ART), 100µU/ml human PR3 protein

(10) Effects of recombinant human soluble thrombomodulin on neutrophil extracellular traps in the kidney of a mouse model of endotoxin shockFujita Med JDecember 27, 2022Tatsuhiko Harada, Yasuyo Shimomura, Osamu Nishida, Munenori Maeda, Yu Kato, Tomoyuki Nakamura, Naohide Kuriyama, Hidefumi Komura(Shino-Test Corporation, Sagamihara, Japan) as previously described.25 citH3 was detected using an ELISA kit (501620; Cayman Chemical, Ann Arbor, MI, USA). Measurement of blood creatinine (CRE) and blood urea nitrogen

(11) Phenotypic alteration of low-density granulocytes in people with pulmonary post-acute sequelae of SARS-CoV-2 infectionFrontiers in ImmunologyDecember 15, 2022Logan S. Dean, Gehan Devendra, Boonyanudh Jiyarom, Natalie Subia, Michelle D. Tallquist, Vivek R. Nerurkar, Sandra P. Chang, Dominic C. Chow, Cecilia M. Shikuma, Juwon Parkplasma using the enzyme-linked immunosorbent assay according to the manufacturer’s instructions (Cayman, 501620). 100 uL of the standard and diluted sample (1:10 in 1x assay buffer) were added to the 96-well pre-coated

(12) Longitudinal characterization of circulating neutrophils uncovers phenotypes associated with severity in hospitalized COVID-19 patientsCell reports. MedicineOctober 18, 2022LaSalle TJ, Gonye ALK, Freeman SS, Kaplonek P, Gushterova I, Kays KR, Manakongtreecheep K, Tantivit J, Rojas-Lopez M, Russo BC, et al.quantified in patient plasma using the Citrullinated Histone H3 (clone 11D3) ELISA kit (Cayman Chemical, 501620) according to manufacturer specifications. Plasma samples (which were previously diluted 1:2 with RPMI)

(13) Complement C3 inhibition in severe COVID-19 using compstatin AMY-101Science advancesAugust 19, 2022Skendros P, Germanidis G, Mastellos DC, Antoniadou C, Gavriilidis E, Kalopitas G, Samakidou A, Liontos A, Chrysanthopoulou A, Ntinopoulou M, et al.plasma were quantified with an H3Cit ELISA kit, according to the instructions of the manufacturer (501620, Cayman Chemical, Ann Arbor, MI, USA). TAT assay Thrombin–anti-thrombin complexes were analyzed in

(14) Neutrophil Extracellular Traps Promote NLRP3 Inflammasome Activation and Glomerular Endothelial Dysfunction in Diabetic Kidney DiseaseNutrientsJuly 20, 2022Gupta A, Singh K, Fatima S, Ambreen S, Zimmermann S, Younis R, Krishnan S, Rana R, Gadi I, Schwab C, et al.performed according to manufacturer’s protocol. H3Cit was measured using Cayman’s Citrullinated H3 kit-501620. Mouse neutrophil elastase (NE) was measured using a commercial ELISA from R&D systems (DY4517-05).

(15) Endothelial Cell–Activating Antibodies in COVID‐19Arthritis & RheumatologyJuly 1, 2022Hui Shi et al.double‐stranded DNA Assay kit (P11496; Invitrogen). Citrullinated histone H3 was quantified by ELISA (501620; Cayman Chemical), and calprotectin was quantified by ELISA (DY8226; R&D Systems). RESULTS Activation

(16) Neutrophil extracellular traps regulate ischemic stroke brain injuryJournal of Clinical InvestigationMay 16, 2022Frederik Denorme, Irina Portier, John L. Rustad, Mark J. Cody, Claudia V. de Araujo, Chieko Hoki, Matthew D. Alexander, Ramesh Grandhi, Mitchell R. Dyer, Matthew D. Neal, et al.were measured using the Citrullinated Histone H3 ELISA Kit according to the manufacturer instructions (501620, Cayman Chemical). MPO-DNA complexes. An in-house ELISA was used to quantify MPO-DNA complexes. Briefly,

(17) Additional risk factors associated with thrombosis and pregnancy morbidity in a unique cohort of antiphospholipid antibody-positive patientsChinese Medical JournalMarch 20, 2022Chun Li, Yu Zuo, Song Zhang, Una E. Makris, David R. Karp, Zhanguo Lihistone H3 (Cit-H3): Cell-free DNA (Invitrogen, Thermo Fisher Scientific, P11496, USA) and Cit-H3 (Cayman, 501620) were quantified in sera according to the manufacturer's instructions. Serum samples were obtained from

(18) Ex Vivo and In Vitro Analysis Identify a Detrimental Impact of Neutrophil Extracellular Traps on Eye Structures in Equine Recurrent UveitisFrontiers in ImmunologyFebruary 10, 2022Leonie Fingerhut, Leyla Yücel, Katrin Strutzberg-Minder, Maren von Köckritz-Blickwede, Bernhard Ohnesorge, Nicole de BuhrELISA Samples were analyzed with a Citrullinated Histone H3 (Clone 11D3) ELISA Kit (Cayman Chemical 501620) following the manufacturer’s instructions. Serum samples were diluted 1:4, VBF samples 1:2 before measurement. IL-17

(19) Zinc-dependent histone deacetylases drive neutrophil extracellular trap formation and potentiate local and systemic inflammationiScienceNovember 19, 2021Valentina Poli et al.assays ,, Quant-iT PicoGreen,Invitrogen,Cat# P11495 Citrullinated histone H3 ELISA kit,Cayman Chemical,Cat# 501620 Cell Death Detection ELISA,Sigma,Cat# 11544675001 TNF ELISA kit,BioLegend,Cat# 430901 IL-6 ELISA

(20) Effects of Neutrophil Extracellular Traps in Patients With Septic Coagulopathy and Their Interaction With AutophagyFrontiers in ImmunologyOctober 11, 2021Jia-Yu Mao, Jia-Hui Zhang, Wei Cheng, Jian-Wei Chen, Na Cuiassessed by enzyme-linked immunosorbent assay (ELISA) (ELA2: abbexa abx196622, Beijing, China; H3: Cayman 501620, Shanghai, China; syndecan-1: CUSABIO CSB-E14983 h, Shanghai, China; mTOR: Abcam ab45996, Shanghai,

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Citrullinated Histone H3 (Clone 11D3) ELISA Kit from Cayman Chemical

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Citrullinated Histone H3 (Clone 11D3) ELISA Kit from Cayman Chemical

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