Fig 1: Effect of SeNPs/ß-glucan concentration on immunity factors in spleen of immunosuppressed mice. (a–f) are spleen, IgM, IgG, TFN-a, IL-2 and IFN-? indexes, respectively; N-Ctrl: Normal control mice received only distilled water; CTX-Ctrl: CTX-induced immunosuppressive mice received only distilled water; in supplemented groups, mice were orally supplied with 0 (only ß-glucan without SeNPs), 2, 4 and 6 mg SeNPs kg-1. Significant differences were compared with the CTX-Ctrl. ns, not significantly different at p > 0.05; *, significant different at p < 0.05; **, significant different at p = 0.01, *** significant different at p < 0.01; ****, significant different at p < 0.001. Data were statistically analyzed using the ANOVA test and expressed as means ± SD, n = 3.
Fig 2: Effect of the TSPO ligands on the pro-inflammatory cytokine IL-2. BV-2 cells were exposed to 100 ng/mL of LPS for 24 h with or without our novel TSPO ligands compared to PK 11,195 (25 µM each). IL-2 levels (pg/mL) were calculated using a standard calibration curve and are presented as mean ± SD; four replicates in each. ANOVA followed by Bonferroni’s post-hoc test was performed. *** p < 0.001 compared to all other groups.
Fig 3: Anti-inflammatory and antioxidant activities of puerarin in rats with bone graft defects. (A) Pro-inflammatory cytokines TNF-a, IL-1ß, IL-17A, IL-6 and TGF-ß1 in the serum of rats with bone grafts following treatment with puerarin or PBS. (B) Anti-inflammatory cytokines IL-2 and IL-10 in the puerarin and PBS groups in rats with bone grafts following treatment with puerarin or PBS. (C) Puerarin administration decreased serum ALT, AST, ?-GT, ALP, DBIL and TBIL levels in experimental rats. (D) Antioxidant activity in bone tissue of rats with bone graft defects following treatment with puerarin or PBS. Data are expressed as the mean ± standard deviation. Each experiment was repeated at least three times. Student's t-test was used to evaluate the statistical significance of differences between two groups. *P<0.05 and **P<0.01 vs. PBS. TNF, tumor necrosis factor; IL, interleukin; TGF, transforming growth factor; ALT, alanine transaminase; AST, glutamic oxaloacetic transaminase; ?-GT, ?-glutamyl transferase; ALP, alkaline phosphatase; DBIL, direct bilirubin; TBIL, total bilirubin.
Fig 4: The relative fractions of arginase-1- and TGF-ß-producing CD11b-negative and CD11b-positive cells. (A, B) Representative flow cytometry contour plot and cumulative composite data of the frequencies of TGF-ß- and arginase-1-producing CD11b+ cells (n = 7). (C, D) Representative flow cytometry dot plots and cumulative composite data of the frequencies of TNF-a- and arginase-1-producing CD11b- cells (n = 7). (E) Representative flow cytometry contour plot and cumulative composite data of the reactive oxygen species (ROS)-producing cells (n = 5). (F) Representative flow cytometry histogram of ROS and the cumulative composite data of mean fluorescence intensity (MFI). (G) The serum IL-2 (n = 8) was detected by ELISA. Each point represents data from an individual mouse. *P < 0.05, **P < 0.01, ***P < 0.001, NS, no significance.
Supplier Page from Abcam for Mouse IL-2 ELISA Kit