Fig 1: BMP7 affects the IDD of T1DM rats by regulating NLRP3 inflammasome activity and NPC pyroptosis. STZ-induced T1DM rats were treated with oe-BMP7 alone or combined with 4’MR (n = 12). A Measurement of disc height of STZ-induced T1DM rats. B HE staining of NP tissues from STZ-induced T1DM rats. C Safranin O-fast green staining for NP tissues and AF tissues of STZ-induced T1DM rats. D ECM-related gene expression in NP tissues from STZ-induced T1DM rats. E BMP7 expression in NP tissues of STZ-induced T1DM rats determined by RT-qPCR. F BMP7 levels in NP tissues of STZ-induced T1DM rats measured by ELISA. G Levels of pyroptosis-related proteins in NP tissues of STZ-induced T1DM rats measured by ELISA. H Apoptosis in NP tissues of STZ-induced T1DM rats detected by TUNEL staining. I Ki67-positive cells in NP tissues of STZ-induced T1DM rats detected by IHC. *p < 0.05. Data are shown as the mean ± standard deviation. Data comparisons among multiple groups were analyzed by one-way ANOVA with Tukey’s post hoc test
Fig 2: Effect of BMP7 on the IDD of T1DM rats by regulating NLRP3 inflammasome activity and pyroptosis of NPCs. A BMP7 expression in the transcriptome sequencing data of T1DM-induced IDD rats. Control (normal rats) n = 3, STZ (T1DM-induced IDD rats) n = 3. B BMP7 expression in the NP tissues of STZ-induced T1DM rats measured by RT-qPCR. C BMP7 expression in the NPCs isolated from STZ-induced T1DM rats measured by RT-qPCR. D Levels of pyroptosis-related proteins in NPCs and cell supernatant measured by ELISA. E BMP7 expression in NPCs from STZ-induced T1DM rats transduced with oe-BMP7 and treated with 4’MR determined by RT-qPCR. F BMP7 levels in NPCs and cell supernatant from STZ-induced T1DM rats transduced with oe-BMP7 and treated with 4’MR measured by ELISA. G Levels of pyroptosis-related proteins in NPCs from STZ-induced T1DM rats transduced with oe-BMP7 and treated with 4’MR measured by ELISA. n = 12. *p < 0.05. n.s. indicates not significant. Data are shown as the mean ± standard deviation. Data between two groups were compared by unpaired t-test, and data comparisons among multiple groups were analyzed by the one-way ANOVA with Tukey’s post hoc test. The cell experiment was repeated three times independently
Fig 3: Molecular mechanism of BMP7 involved in the IDD of T1DM rats. BMP7 inhibits NPC pyroptosis and NLRP3 inflammasome activity to ameliorate IDD in T1DM
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Rat BMP-7 ELISA Kit (Colorimetric)