Fig 1: Competitiveness of oligosaccharides and HA in binding to CD44. (A) Reducing effect of oligosaccharides on CD44 antigenicity. Recombinant rat CD44 (20 ng/mL) was mixed with or without FC, FOS, or GOS for 10 min and then analyzed using the CD44 ELISA kit. Results are expressed as mean ± SD (n = 4). *, p < 0.05 vs. the control group. (B) HA interfering with the inhibitory effect of oligosaccharides on CD44 and phosphorylated JNK expression. NRK-52E cells were pretreated with 0.1 mg/mL HA for 30 min, then administered with 0.1 mg/mL FC, FOS, or GOS for 30 min and finally subjected to hypoxic culture. Protein expression was analyzed by Western blot. Relative increases in the protein bands are also presented in bar chart form. Results are expressed as mean ± SD (n = 4).
Fig 2: Inhibitory effect of oligosaccharides on CD44 signal transduction in hypoxic NRK-52E cells. The cells were pretreated with FC, FOS, or GOS for 30 min and then subjected to hypoxic culture. Protein expression was analyzed by Western blotting. (A) Dose-dependent effects of oligosaccharides on CD44 expression. (B) Effect of oligosaccharides on CD44-related signals. Oligosaccharide concentration was set as 0.1 mg/mL. Relative increases in protein bands are also presented in bar chart form (C). Results are expressed as mean ± SD (n = 4).
Fig 3: Role of CD44 and JNK in hypoxia-induced inflammatory responses in NRK-52E cells. Protein expression was analyzed by Western blot. (A) Inhibitory effect of CD44 siRNA transfection on phosphorylated JNK and cytokines. (B) Inhibitory effect of SP600125 on cytokines. The cells were pretreated with 20 μM SP600125 for 30 min prior to hypoxia treatment. Relative increases in protein bands are also presented in bar chart form. Results are expressed as mean ± SD (n = 4).
Supplier Page from Abcam for Rat CD44 ELISA Kit