Fig 1: Survival analysis of hub genes in GSE65682. (A–G) The survival curves of the 7 hub genes in GSE65682. Patients with high expression of FOLR3 and OSCAR had a better prognosis than low expression of these genes. Patients with low expression of MMP8 and ARG1 had a better prognosis than high expression of these genes.
Fig 2: Site-level measurements of MMP-8 (a) and Lactoferrin (b) of aMMP-8 versus probing pocket depth (PPD)
Fig 3: Schematic summary of the in silico and in vitro study of sepsis serum promotes leukocyte adhesion to HUVEC via MMP8.
Fig 4: Expression of MMP8 in blood samples from sepsis patients and controls. (A) mRNA expression of MMP8 in neutrophil of patients and controls was measured by q-RTPCR. (B) Protein level of MMP8 in serum of patients and controls was detected by ELISA. *p < 0.05 between control and sepsis group by using t-test.
Fig 5: Identification and GO functional analysis of DEGs and hub genes in GSE64457. (A) The top 100 most significant DEGs are shown in the heatmap (|log2FC| > 1 and adj. p < 0.05). Red indicates a high expression, while green indicates a relatively low expression. (B) The volcano plot shows the 429 DEGs that containing 266 upregulated genes in red (log2FC > 1 and adj. p < 0.05) and 163 downregulated genes in green (log2FC < −1 and adj. p < 0.05). Hub genes are highlighted in the plot. (C) Shows the PPI network of DEGs. Red notes the upregulated genes and green noted the downregulated genes. Triangles represent the hub genes in the network. (D) Hub genes (MMP8, HP, ARG1, FOLR3, QSOX1, PGLYRP1, OSCAR) were identified by the overlapping of three algorithms (MNC, MCC, and Degree) in the cytoHubba plugin in Cytoscape and clustered in heatmap (E). The top 15 GO terms are shown in the dot plots of GO functional analysis of DEGs (F) and hub genes (G) (p < 0.05). The size of each circle is positive correlated with the counts of the enriched genes in this term. GO, gene ontology; DEGs, differentially expressed genes; PPI, protein-protein interaction; MMP8, matrix metallopeptidase 8; HP, haptoglobin; ARG1, arginase 1; FOLR3, folate receptor gamma; QSOX1, quiescin sulfhydryl oxidase 1; PGLYRP1, peptidoglycan recognition protein 1; OSCAR, osteoclast associated Ig-like receptor; MNC, maximum neighborhood component; MCC, maximal clique centrality.
Supplier Page from Abcam for Human MMP8 ELISA Kit