Fig 1: Isotype and subclass distribution within vaccine-induced MERS-CoV-S-specific antibodiesS1- (A) and S2- (B) specific responses of IgM, IgG1–4, and IgA1–2 at different time points after vaccination, displayed as fold changes of median fluorescence intensities (MFIs; measured by bead-based ELISA) compared with baseline values at V1:day 0. Data are represented as individual data points (mean of technical duplicates) and median ± IQR. Number of samples and median fold changes are shown in Table S3.
Fig 2: Correlation between vaccine-induced antibody and B cell responsesCorrelation analysis of MERS-CoV-S1- (A) and -S2- (B) specific IgG1, IgG3, and ASBC responses at different time points after vaccinations 1, 2, and 3 (V1, V2, and V3). S-specific MBC responses were included into correlograms of both the S1 and S2 antigen. Positive correlations are shown in blue and negative correlations in red, as indicated by the color bar. Sample numbers included into correlation analysis are provided in Table S7.
Fig 3: Longitudinal dynamics of MERS-CoV-S-specific IgG1 and IgG3 antibodiesVaccine-induced S1/S2-specific IgG1 (A) and IgG3 (B) are displayed as MFI, measured by bead-based multiplex ELISA. Data are represented as individual data points (mean of technical duplicates) and median ± IQR. LLD, lower limit of detection. Number of samples, median, and p values for each time point are shown in Table S4.
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