rtStar™ tRF&tiRNA Pretreatment Kit AS-FS-005 from Arraystar, Inc

Product Specs
ItemrtStar™ tRF&tiRNA Pretreatment Kit
CompanyArraystar, Inc
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAS-FS-005
ApplicationsPCR
TypetRF&tiRNA
Quantity12RXNs

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Arraystar, Inc

9430 Key West Avenue

Suite 128

Rockville, Maryland 20850

United States

Phone: 888-416-6343
240-314-0388
Fax: 240-238-9860

Company Profile

Website: http://www.arraystar.com

(1) XPOT Disruption Suppresses TNBC Growth through Inhibition of Specific tRNA Nuclear Exportation and TTC19 Expression to Induce Cytokinesis FailureInternational Journal of Biological SciencesOctober 24, 2023Huijuan Dai, Xiaomei Yang, Xiaonan Sheng, Yaohui Wang, Shan Zhang, Xueli Zhang, Lipeng Hu, Zhigang Zhang, Xinrui Dong, Wenjin Yin, et al.size window of 60-100 nt for tRNA. The “demethylation” section in rtStarTM tRF&tiRNA Pretreatment Kit (AS-FS-005; Arraystar) was used for tRNA m1A&m3C demethylation treatment. A 50-µL demethylation reaction mixture

(2) Expression profiles and function prediction of tRNA-derived fragments in gliomaBMC CancerOctober 20, 2023Deng Wei, Ben Niu, Bei Zhai, Xiao-bai Liu, Yi-long Yao, Chan-chan Liang, Ping Wangmanufacturer instructions. Some RNA modifications were removed using rtStarTM tRF&tiRNA Pretreatment Kit (Cat# AS-FS-005, Arraystar). Using 2×miRNA P-RT Solution mix (Shanghai Sangon Biotech, China) and miRNA P-RT Enzyme

(3) Hyperglycemia-regulated tRNA-derived fragment tRF-3001a propels neurovascular dysfunction in diabetic miceCell Reports MedicineOctober 1, 2023Jun-Ya Zhu, Wen Yao, Xi-Sen Ni, Mu-Di Yao, Wen Bai, Tian-Jing Yang, Zi-Ran Zhang, Xiu-Miao Li, Qin Jiang, Biao Yancommercial assays,Critical commercial assays ,, rtStar™ tRF and tiRNA pretreatment kit,Arraystar,Cat# AS-FS-005 rtStar™ First-strand cDNA Synthesis kit,Arraystar,Cat# AS-FS-003 SuperScript IV First-Strand Synthesis System

(4) Differential expression spectrum and targeted gene prediction of tRNA-derived small RNAs in idiopathic pulmonary arterial hypertensionFrontiers in Molecular BiosciencesJuly 11, 2023Yusi Chen, Yi Tang, Sitong Hou, Jun Luo, Jingyuan Chen, Haihua Qiu, Wenjie Chen, Kexing Li, Jin He, Jiang Liagarose gel electrophoresis. RNA samples were preconditioned using the rtStar tsRNA Pretreatment kit (Cat# AS-FS-005, Arraystar) to strip RNA of modifications such as acetylation and methylation that would interfere with

(5) 5’tiRNA-Pro-TGG, a novel tRNA halve, promotes oncogenesis in sessile serrated lesions and serrated pathway of colorectal cancerWorld Journal of Gastrointestinal OncologyJune 15, 2023Xin-Yuan Wang, Yu-Jie Zhou, Hai-Ying Chen, Jin-Nan Chen, Shan-Shan Chen, Hui-Min Chen, Xiao-Bo Limentioned before. Next, a commercial RNA pretreatment kit (rtStar™ tRF and tiRNA Pretreatment Kit, AS-FS-005, Arraystar Inc., MD, United States) for tRF and tiRNA-seq library preparation was used, which was then

(6) Systematic and Comprehensive Analysis of tRNA-Derived Small RNAs Reveals Their Potential Regulatory Roles and Clinical Relevance in SarcoidosisJournal of Inflammation ResearchJune 1, 2023Min Zhao, Chang Tian, Xin Di, Shan Cong, Yingshu Cao, Xijia Zhou, Ke WangTRIzol® reagent. RNA samples were pre-treated using the rtStar™ tRF and tiRNA Pre-treatment Kit (cat. no. AS‑FS‑005; Arraystar Inc.) to remove RNA modifications, and then reverse transcribed to cDNA using the rtStar™ First-Strand cDNA Synthesis Kit

(7) Expression profiles and potential roles of serum tRNA‑derived fragments in diabetic nephropathyExperimental and Therapeutic MedicineMay 11, 2023Chan Huang, Ling Ding, Jialing Ji, Yunyang Qiao, Zihuan Xia, Huimin Shi, Shiting Zhang, Weihua Gan, Aiqing Zhangserum of patients with DN and DM was treated using the rtStar™ tRF&tiRNA Pretreatment Kit (cat. no. AS-FS-005; Arraystar, Inc.) to remove excess modifications and ensure efficient RT. The 3' and 5' miRNA adapters

(8) Men who inject opioids exhibit altered tRNA-Gly-GCC isoforms in semenMolecular Human ReproductionFebruary 28, 2023Germán Gornalusse, Ryan M Spengler, Erin Sandford, Yeseul Kim, Claire Levy, Muneesh Tewari, Florian Hladik, Lucia Vojtechdescribed above was submitted to ArrayStar PCR service. An rtStar™ tRF&tiRNA Pretreatment Kit (Cat# AS-FS-005, Arraystar, Rockville, MD, USA) was used for RNA sample pretreatment; this process removed chemical

(9) Identification of a tsRNA Contributor to Impaired Diabetic Wound Healing via High Glucose-Induced Endothelial DysfunctionDiabetes, Metabolic Syndrome and ObesityJanuary 31, 2023Xiao-Tian Zhang, Zhen-Yang Mao, Xiang-Yun Jin, Yu-Gang Wang, Yu-Qi Dong, Chao ZhangtsRNAs. qRT-PCR Analyses For tsRNAs, RNAs were prepared using the rtStar™ tRFandtiRNA Pretreatment Kit (Cat# AS-FS-005, Arraystar) and then reverse transcribed into first-strand cDNA and amplified to cDNA using the rtStar™ First-Strand cDNA Synthesis Kit (3’ and 5’ adaptor

(10) Differentially expressed tRNA-derived fragments and their roles in primary cardiomyocytes stimulated by high glucoseFront Endocrinol (Lausanne)January 13, 2023Yongting Zhao, Ruxin Wang, Qi Qin, Jiaojiao Yu, Hui Che, Lihong Wangusing a RNeasy® MinEluteTM cleanup kit (Qiagen, Germany). The rtStarTM tRF&tiRNA pretreatment kit (Cat# AS-FS-005, Arraystar, USA) and rtStarTM First-Strand cDNA Synthesis kit (Cat# AS-FS-003, Arraystar, USA) were

(11) Inhibition of tiRNA-Gly-GCC ameliorates neointimal formation via CBX3-mediated VSMCs phenotypic switchingFrontiers in cardiovascular medicineJanuary 1, 2023Rong Z, Li F, Zhang R, Niu S, Di X, Ni L, Liu C.were performed as previously described (34). Briefly, the rtStar™ tRF and tiRNA Pretreatment Kit (Cat# AS-FS-005, Arraystar, Rockville, MD, USA) was used to remove RNA modifications, and the rtStar™ First-Strand cDNA Synthesis Kit (3′ and 5′ adaptor

(12) Role of tRNA derived fragments in renal ischemia–reperfusion injuryRenal FailureDecember 31, 2022Dan Li, Hao Zhang, Xueqin Wu, Qing Dai, Shiqi Tang, Yan Liu, Shikun Yang, Wei Zhangthe concentration and purity of each RNA sample. Next, Using rtStar™ tRF&tiRNA Pretreatment Kit (Cat# AS-FS-005, Arraystar) to remove the modification of total RNA. Then, the RNA was reversed to cDNA. Next, polymerase chain reaction

(13) tRF3008A suppresses the progression and metastasis of colorectal cancer by destabilizing FOXK1 in an AGO-dependent mannerJournal of Experimental & Clinical Cancer ResearchDecember 1, 2022Ying Han, Yinghui Peng, Shanshan Liu, Xinwen Wang, Changjing Cai, Cao Guo, Yihong Chen, Le Gao, Qiaoqiao Huang, Min He, et al.South University. tRFs pretreatment and cDNA synthesis The rtStar™ tRF&tiRNA Pretreatment Kit (Cat# AS-FS-005, Arraystar) was used to remove the modifications that interfere with small RNA-seq library construction

(14) RNA â€sequencing reveals the expression profiles of tsRNAs and their potential carcinogenic role in cholangiocarcinomaJournal of Clinical Laboratory AnalysisOctober 1, 2022Yanâ€ke Li, Liâ€rong Yan, Ang Wang, Liâ€yue Jiang, Qian Xu, Benâ€gang Wangsamples by TRIZOL (TIANGEN BIOTECH). Total RNA was pretreated by rtStar™ tRF&tiRNA Pretreatment Kit (Cat# AS‐FS‐005). cDNA was synthesized with the rtStar™ First‐Strand cDNA Synthesis Kit (3' and 5' adaptor) (Cat#AS‐FS‐003‐02;

(15) A Novel tiRNA-Gly-GCC-1 Promotes Progression of Urothelial Bladder Carcinoma and Directly Targets TLR4Cancers (Basel)September 20, 2022Chuan Qin, Zheng-Hao Chen, Rui Cao, Ming-Jun Shi, Ye Tiandemethylation were performed for tiRNA-Gly-GCC-1 using the rtStar™ tRF&tiRNA Pretreatment Kit (Cat# AS-FS-005, Arraystar). Next, complementary DNA (cDNA) was synthesized using the rtStar™ First-Strand cDNA Synthesis Kit

(16) Differential Expression Profiles and Bioinformatics Analysis of tRNA-Derived Small RNAs in Muscle-Invasive Bladder Cancer in a Chinese PopulationGenes (Basel)March 28, 2022Chuan Qin, Zheng-Hao Chen, Rui Cao, Ming-Jun Shi, Ye Tianpretreatment and cDNA synthesis were performed using rtStarTM tRF and tiRNA Pretreatment Kit (Cat# AS-FS-005, Arraystar, Rockville, MD, USA) and rtStarTM First-Strand cDNA Synthesis Kit (Cat# AS-FS-003, Arraystar),

(17) Characteristics of Transfer RNA-Derived Fragments Expressed during Human Renal Cell Development: The Role of Dicer in tRF BiogenesisInternational Journal of Molecular SciencesMarch 26, 2022Marek Kazimierczyk, Marta Wojnicka, Ewa Biała, Paulina Żydowicz-Machtel, Barbara Imiołczyk, Tomasz Ostrowski, Anna Kurzyńska-Kokorniak, Jan Wrzesinskisubsequent steps, the total RNA was pretreated using the rtStarTM tRF and tiRNA Pretreatment Kit (cat. no. AS-FS-005, Arraystar, Rockville, MD, USA), in accordance with the manufacturer’s instructions. The second small

(18) Expression profiles of tRNA-derived fragments and their potential roles in lung adenocarcinomaAnnals of Translational MedicineFebruary 1, 2022Le-Tian Huang, Meng Cui, Mario Silva, Katsuhiro Okuda, Yoshihisa Shimada, Jia-He Wang, Yi-Bing Wangagarose gel electrophoresis. Pretreatment of tRFs and tiRNAs and library preparation The rtStar™ tRF&tiRNA Pretreatment Kit (Catalog NO. AS-FS-005, Arraystar, MD, USA) was utilized to treat total RNA samples. According to the manufacturer’s instructions,

(19) tRNA-derived fragment TRF365 regulates the metabolism of anterior cruciate ligament cells by targeting IKBKBCell death discoveryJanuary 10, 2022Long D, Xu Y, Mao G, Xin R, Deng Z, Liao H, Li Z, Yang Z, Yu B, Yang Z, et al.using the PrimeScript RT master mix (Takara, Japan). The rtStar tRF & tiRNA Pretreatment Kit (Cat. #AS-FS-005; Arraystar) was used for miRNA sample pretreatment. The rtStar First-Strand cDNA Synthesis Kit with

(20) tRNA-Derived Fragment tRF-5009A Regulates Autophagy and Degeneration of Cartilage in Osteoarthritis via Targeting mTOROxidative medicine and cellular longevityJanuary 1, 2022Deng Z, Long D, Liu H, Xu Y, Xin R, Liao H, Li Z, Li R, Mao G, Zhang Z, et al.NanoDrop spectrophotometer (NanoDrop Technologies, USA). The rtStar tRF and tiRNA pretreatment kit (Cat. #AS-FS-005; Arraystar) was used to preprocess the miRNA samples. cDNA was synthesized using the rtStar first-strand cDNA synthesis kit

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rtStar™ tRF&tiRNA Pretreatment Kit from Arraystar, Inc

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rtStar™ tRF&tiRNA Pretreatment Kit from Arraystar, Inc

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