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Assay Kits
Assay Kit
rtStar™ tRF&tiRNA Pretreatment Kit
rtStar™ tRF&tiRNA Pretreatment Kit from Arraystar, Inc
Be the first to write a review!
Citations:
(37)
Supplier Page
Supplier Page from
Arraystar, Inc for
rtStar™ tRF&tiRNA Pretreatment Kit
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Description
rtStar™ tRF&tiRNA Pretreatment Kit is designed to remove the heavy modifications in tRF & tiRNA and prepare the RNA samples before tRF&tiRNA cDNA library construction of qPCR
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
rtStar™ tRF&tiRNA Pretreatment Kit
Company
Arraystar, Inc
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
AS-FS-005
Applications
PCR
Type
tRF&tiRNA
Quantity
12RXNs
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Arraystar, Inc
9430 Key West Avenue
Suite 128
Rockville
,
Maryland
20850
United States
Phone
: 888-416-6343
240-314-0388
Fax: 240-238-9860
Company Profile
Website:
http://www.arraystar.com
Citations (37)
(1)
XPOT Disruption Suppresses TNBC Growth through Inhibition of Specific tRNA Nuclear Exportation and TTC19 Expression to Induce Cytokinesis Failure
International Journal of Biological Sciences
October 24, 2023
Huijuan Dai, Xiaomei Yang, Xiaonan Sheng, Yaohui Wang, Shan Zhang, Xueli Zhang, Lipeng Hu, Zhigang Zhang, Xinrui Dong, Wenjin Yin,
et al.
size window of 60-100 nt for tRNA. The “demethylation” section in rtStarTM tRF&tiRNA Pretreatment Kit (
AS-FS-005
;
Arraystar
) was used for tRNA m1A&m3C demethylation treatment. A 50-µL demethylation reaction mixture
(2)
Expression profiles and function prediction of tRNA-derived fragments in glioma
BMC Cancer
October 20, 2023
Deng Wei, Ben Niu, Bei Zhai, Xiao-bai Liu, Yi-long Yao, Chan-chan Liang, Ping Wang
manufacturer instructions. Some RNA modifications were removed using rtStarTM tRF&tiRNA Pretreatment Kit (Cat#
AS-FS-005
,
Arraystar
). Using 2×
miRNA
P-RT Solution mix (Shanghai Sangon Biotech, China) and
miRNA
P-RT Enzyme
(3)
Hyperglycemia-regulated tRNA-derived fragment tRF-3001a propels neurovascular dysfunction in diabetic mice
Cell Reports Medicine
October 1, 2023
Jun-Ya Zhu, Wen Yao, Xi-Sen Ni, Mu-Di Yao, Wen Bai, Tian-Jing Yang, Zi-Ran Zhang, Xiu-Miao Li, Qin Jiang, Biao Yan
commercial
assays
,Critical commercial
assays
,, rtStar™ tRF and tiRNA pretreatment kit,
Arraystar
,Cat#
AS-FS-005
rtStar™ First-strand cDNA Synthesis kit
,
Arraystar
,Cat#
AS-FS-003
SuperScript IV First-Strand Synthesis System
(4)
Differential expression spectrum and targeted gene prediction of tRNA-derived small RNAs in idiopathic pulmonary arterial hypertension
Frontiers in Molecular Biosciences
July 11, 2023
Yusi Chen, Yi Tang, Sitong Hou, Jun Luo, Jingyuan Chen, Haihua Qiu, Wenjie Chen, Kexing Li, Jin He, Jiang Li
agarose gel electrophoresis
. RNA samples were preconditioned using the rtStar tsRNA Pretreatment kit (Cat#
AS-FS-005
,
Arraystar
) to strip RNA of modifications such as acetylation and methylation that would interfere with
(5)
5’tiRNA-Pro-TGG, a novel tRNA halve, promotes oncogenesis in sessile serrated lesions and serrated pathway of colorectal cancer
World Journal of Gastrointestinal Oncology
June 15, 2023
Xin-Yuan Wang, Yu-Jie Zhou, Hai-Ying Chen, Jin-Nan Chen, Shan-Shan Chen, Hui-Min Chen, Xiao-Bo Li
mentioned before. Next, a commercial RNA pretreatment kit (rtStar™ tRF and tiRNA Pretreatment Kit,
AS-FS-005
,
Arraystar Inc
., MD, United States) for tRF and tiRNA-seq library preparation was used, which was then
(6)
Systematic and Comprehensive Analysis of tRNA-Derived Small RNAs Reveals Their Potential Regulatory Roles and Clinical Relevance in Sarcoidosis
Journal of Inflammation Research
June 1, 2023
Min Zhao, Chang Tian, Xin Di, Shan Cong, Yingshu Cao, Xijia Zhou, Ke Wang
TRIzol® reagent. RNA samples were pre-treated using the rtStar™ tRF and tiRNA Pre-treatment Kit (cat. no.
AS‑FS‑005
;
Arraystar Inc
.) to remove RNA modifications, and then reverse transcribed to cDNA using the
rtStar™ First-Strand cDNA Synthesis Kit
(7)
Expression profiles and potential roles of serum tRNA‑derived fragments in diabetic nephropathy
Experimental and Therapeutic Medicine
May 11, 2023
Chan Huang, Ling Ding, Jialing Ji, Yunyang Qiao, Zihuan Xia, Huimin Shi, Shiting Zhang, Weihua Gan, Aiqing Zhang
serum of patients with DN and DM was treated using the
rtStar™ tRF&tiRNA Pretreatment Kit
(cat. no.
AS-FS-005
;
Arraystar, Inc
.) to remove excess modifications and ensure efficient RT. The 3' and 5'
miRNA
adapters
(8)
Men who inject opioids exhibit altered tRNA-Gly-GCC isoforms in semen
Molecular Human Reproduction
February 28, 2023
Germán Gornalusse, Ryan M Spengler, Erin Sandford, Yeseul Kim, Claire Levy, Muneesh Tewari, Florian Hladik, Lucia Vojtech
described above was submitted to ArrayStar
PCR
service. An
rtStar™ tRF&tiRNA Pretreatment Kit
(Cat#
AS-FS-005
,
Arraystar
, Rockville, MD, USA) was used for RNA sample pretreatment; this process removed chemical
(9)
Identification of a tsRNA Contributor to Impaired Diabetic Wound Healing via High Glucose-Induced Endothelial Dysfunction
Diabetes, Metabolic Syndrome and Obesity
January 31, 2023
Xiao-Tian Zhang, Zhen-Yang Mao, Xiang-Yun Jin, Yu-Gang Wang, Yu-Qi Dong, Chao Zhang
tsRNAs.
qRT-PCR
Analyses For tsRNAs, RNAs were prepared using the rtStar™ tRFandtiRNA Pretreatment Kit (Cat#
AS-FS-005
,
Arraystar
) and then reverse transcribed into first-strand cDNA and amplified to cDNA using the
rtStar™ First-Strand cDNA Synthesis Kit (3’ and 5’ adaptor
(10)
Differentially expressed tRNA-derived fragments and their roles in primary cardiomyocytes stimulated by high glucose
Front Endocrinol (Lausanne)
January 13, 2023
Yongting Zhao, Ruxin Wang, Qi Qin, Jiaojiao Yu, Hui Che, Lihong Wang
using a RNeasy® MinEluteTM cleanup kit (Qiagen, Germany). The rtStarTM tRF&tiRNA pretreatment kit (Cat#
AS-FS-005
,
Arraystar
, USA) and rtStarTM
First-Strand cDNA Synthesis kit
(Cat#
AS-FS-003
,
Arraystar
, USA) were
(11)
Inhibition of tiRNA-Gly-GCC ameliorates neointimal formation via CBX3-mediated VSMCs phenotypic switching
Frontiers in cardiovascular medicine
January 1, 2023
Rong Z, Li F, Zhang R, Niu S, Di X, Ni L, Liu C.
were performed as previously described (34). Briefly, the rtStar™ tRF and tiRNA Pretreatment Kit (Cat#
AS-FS-005
,
Arraystar
, Rockville, MD, USA) was used to remove RNA modifications, and the
rtStar™ First-Strand cDNA Synthesis Kit (3′ and 5′ adaptor
(12)
Role of tRNA derived fragments in renal ischemia–reperfusion injury
Renal Failure
December 31, 2022
Dan Li, Hao Zhang, Xueqin Wu, Qing Dai, Shiqi Tang, Yan Liu, Shikun Yang, Wei Zhang
the concentration and purity of each RNA sample. Next, Using
rtStar™ tRF&tiRNA Pretreatment Kit
(Cat#
AS-FS-005
,
Arraystar
) to remove the modification of total RNA. Then, the RNA was reversed to cDNA. Next,
polymerase chain reaction
(13)
tRF3008A suppresses the progression and metastasis of colorectal cancer by destabilizing FOXK1 in an AGO-dependent manner
Journal of Experimental & Clinical Cancer Research
December 1, 2022
Ying Han, Yinghui Peng, Shanshan Liu, Xinwen Wang, Changjing Cai, Cao Guo, Yihong Chen, Le Gao, Qiaoqiao Huang, Min He,
et al.
South University. tRFs pretreatment and
cDNA synthesis
The
rtStar™ tRF&tiRNA Pretreatment Kit
(Cat#
AS-FS-005
,
Arraystar
) was used to remove the modifications that interfere with small RNA-seq
library construction
(14)
RNA â€sequencing reveals the expression profiles of tsRNAs and their potential carcinogenic role in cholangiocarcinoma
Journal of Clinical Laboratory Analysis
October 1, 2022
Yanâ€ke Li, Liâ€rong Yan, Ang Wang, Liâ€yue Jiang, Qian Xu, Benâ€gang Wang
samples by TRIZOL (TIANGEN BIOTECH). Total RNA was pretreated by
rtStar™ tRF&tiRNA Pretreatment Kit
(Cat#
AS‐FS‐005
). cDNA was synthesized with the
rtStar™ First‐Strand cDNA Synthesis Kit (3' and 5' adaptor
) (Cat#
AS‐FS‐003
‐02;
(15)
A Novel tiRNA-Gly-GCC-1 Promotes Progression of Urothelial Bladder Carcinoma and Directly Targets TLR4
Cancers (Basel)
September 20, 2022
Chuan Qin, Zheng-Hao Chen, Rui Cao, Ming-Jun Shi, Ye Tian
demethylation were performed for tiRNA-Gly-GCC-1 using the
rtStar™ tRF&tiRNA Pretreatment Kit
(Cat#
AS-FS-005
,
Arraystar
). Next, complementary DNA (cDNA) was synthesized using the
rtStar™ First-Strand cDNA Synthesis Kit
(16)
Differential Expression Profiles and Bioinformatics Analysis of tRNA-Derived Small RNAs in Muscle-Invasive Bladder Cancer in a Chinese Population
Genes (Basel)
March 28, 2022
Chuan Qin, Zheng-Hao Chen, Rui Cao, Ming-Jun Shi, Ye Tian
pretreatment and
cDNA synthesis
were performed using rtStarTM tRF and tiRNA Pretreatment Kit (Cat#
AS-FS-005
,
Arraystar
, Rockville, MD, USA) and rtStarTM
First-Strand cDNA Synthesis Kit
(Cat#
AS-FS-003
,
Arraystar
),
(17)
Characteristics of Transfer RNA-Derived Fragments Expressed during Human Renal Cell Development: The Role of Dicer in tRF Biogenesis
International Journal of Molecular Sciences
March 26, 2022
Marek Kazimierczyk, Marta Wojnicka, Ewa Biała, Paulina Żydowicz-Machtel, Barbara Imiołczyk, Tomasz Ostrowski, Anna Kurzyńska-Kokorniak, Jan Wrzesinski
subsequent steps, the total RNA was pretreated using the rtStarTM tRF and tiRNA Pretreatment Kit (cat. no.
AS-FS-005
,
Arraystar
, Rockville, MD, USA), in accordance with the manufacturer’s instructions. The second small
(18)
Expression profiles of tRNA-derived fragments and their potential roles in lung adenocarcinoma
Annals of Translational Medicine
February 1, 2022
Le-Tian Huang, Meng Cui, Mario Silva, Katsuhiro Okuda, Yoshihisa Shimada, Jia-He Wang, Yi-Bing Wang
agarose gel electrophoresis
. Pretreatment of tRFs and tiRNAs and library preparation The
rtStar™ tRF&tiRNA Pretreatment Kit
(Catalog NO.
AS-FS-005
,
Arraystar
, MD, USA) was utilized to treat total RNA samples. According to the manufacturer’s instructions,
(19)
tRNA-derived fragment TRF365 regulates the metabolism of anterior cruciate ligament cells by targeting IKBKB
Cell death discovery
January 10, 2022
Long D, Xu Y, Mao G, Xin R, Deng Z, Liao H, Li Z, Yang Z, Yu B, Yang Z,
et al
.
using the PrimeScript RT master mix (Takara, Japan). The
rtStar tRF & tiRNA Pretreatment Kit
(Cat. #
AS-FS-005
;
Arraystar
) was used for
miRNA
sample pretreatment. The
rtStar First-Strand cDNA Synthesis Kit
with
(20)
tRNA-Derived Fragment tRF-5009A Regulates Autophagy and Degeneration of Cartilage in Osteoarthritis via Targeting mTOR
Oxidative medicine and cellular longevity
January 1, 2022
Deng Z, Long D, Liu H, Xu Y, Xin R, Liao H, Li Z, Li R, Mao G, Zhang Z,
et al
.
NanoDrop spectrophotometer (NanoDrop Technologies, USA). The rtStar tRF and tiRNA pretreatment kit (Cat. #
AS-FS-005
;
Arraystar
) was used to preprocess the
miRNA
samples. cDNA was synthesized using the
rtStar first-strand cDNA synthesis kit
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rtStar™ tRF&tiRNA Pretreatment Kit from Arraystar, Inc
Supplier Page
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