rtStar™ First-Strand cDNA Synthesis Kit (3’ and 5’ adaptor) AS-FS-003 from Arraystar, Inc

Product Specs
ItemrtStar™ First-Strand cDNA Synthesis Kit (3’ and 5’ adaptor)
CompanyArraystar, Inc
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAS-FS-003
TypetRF&tiRNA
ApplicationscDNA Synthesis
Quantity6RXNs

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Arraystar, Inc

9430 Key West Avenue

Suite 128

Rockville, Maryland 20850

United States

Phone: 888-416-6343
240-314-0388
Fax: 240-238-9860

Company Profile

Website: http://www.arraystar.com

(1) Hyperglycemia-regulated tRNA-derived fragment tRF-3001a propels neurovascular dysfunction in diabetic miceCell Reports MedicineOctober 1, 2023Jun-Ya Zhu, Wen Yao, Xi-Sen Ni, Mu-Di Yao, Wen Bai, Tian-Jing Yang, Zi-Ran Zhang, Xiu-Miao Li, Qin Jiang, Biao YantiRNA pretreatment kit,Arraystar,Cat# AS-FS-005 rtStar™ First-strand cDNA Synthesis kit,Arraystar,Cat# AS-FS-003 SuperScript IV First-Strand Synthesis System,Thermo Fisher Scientific,Cat# 18091050 BeyoClick™EdU

(2) Differential expression spectrum and targeted gene prediction of tRNA-derived small RNAs in idiopathic pulmonary arterial hypertensionFrontiers in Molecular BiosciencesJuly 11, 2023Yusi Chen, Yi Tang, Sitong Hou, Jun Luo, Jingyuan Chen, Haihua Qiu, Wenjie Chen, Kexing Li, Jin He, Jiang Lireverse transcription as the producer’s protocol using the rtStar First-Strand cDNA Synthesis kit (Cat# AS-FS-003, Arraystar). Real-time PCR was carried out by QuantStudio 5 Real-time PCR System (Applied Biosystems),

(3) Systematic and Comprehensive Analysis of tRNA-Derived Small RNAs Reveals Their Potential Regulatory Roles and Clinical Relevance in SarcoidosisJournal of Inflammation ResearchJune 1, 2023Min Zhao, Chang Tian, Xin Di, Shan Cong, Yingshu Cao, Xijia Zhou, Ke Wangmodifications, and then reverse transcribed to cDNA using the rtStar™ First-Strand cDNA Synthesis Kit (cat. no. AS-FS-003; Arraystar Inc, USA.) according to the manufacturer’s protocol. RT-qPCR was performed using the ViiA

(4) Men who inject opioids exhibit altered tRNA-Gly-GCC isoforms in semenMolecular Human ReproductionFebruary 28, 2023Germán Gornalusse, Ryan M Spengler, Erin Sandford, Yeseul Kim, Claire Levy, Muneesh Tewari, Florian Hladik, Lucia Vojtechextraction and ethanol precipitation, rtStar™ first-strand cDNA synthesis kit (3′ and 5′ adaptor) (Cat# AS-FS-003, Arraystar, Rockville, MD, USA) was used as to synthesize cDNA. Equivalent amounts of cDNA from each

(5) Identification of a tsRNA Contributor to Impaired Diabetic Wound Healing via High Glucose-Induced Endothelial DysfunctionDiabetes, Metabolic Syndrome and ObesityJanuary 31, 2023Xiao-Tian Zhang, Zhen-Yang Mao, Xiang-Yun Jin, Yu-Gang Wang, Yu-Qi Dong, Chao ZhangcDNA and amplified to cDNA using the rtStar™ First-Strand cDNA Synthesis Kit (3’ and 5’ adaptor) (Cat# AS-FS-003, Arraystar), according to the manufacturer’s protocol. For mRNA, reverse transcription into cDNA was

(6) Differentially expressed tRNA-derived fragments and their roles in primary cardiomyocytes stimulated by high glucoseFront Endocrinol (Lausanne)January 13, 2023Yongting Zhao, Ruxin Wang, Qi Qin, Jiaojiao Yu, Hui Che, Lihong Wangpretreatment kit (Cat# AS-FS-005, Arraystar, USA) and rtStarTM First-Strand cDNA Synthesis kit (Cat# AS-FS-003, Arraystar, USA) were applied separately to pretreat tRF and tiRNA and synthetize first-strand cDNA

(7) Inhibition of tiRNA-Gly-GCC ameliorates neointimal formation via CBX3-mediated VSMCs phenotypic switchingFrontiers in cardiovascular medicineJanuary 1, 2023Rong Z, Li F, Zhang R, Niu S, Di X, Ni L, Liu C.remove RNA modifications, and the rtStar™ First-Strand cDNA Synthesis Kit (3′ and 5′ adaptor) (Cat# AS-FS-003, Arraystar, Rockville, MD, USA) was used to synthesize cDNA. For mRNA reverse transcription, cDNA was

(8) tRF3008A suppresses the progression and metastasis of colorectal cancer by destabilizing FOXK1 in an AGO-dependent mannerJournal of Experimental & Clinical Cancer ResearchDecember 1, 2022Ying Han, Yinghui Peng, Shanshan Liu, Xinwen Wang, Changjing Cai, Cao Guo, Yihong Chen, Le Gao, Qiaoqiao Huang, Min He, et al.efficient reverse transcription. The rtStar™ First-Strand cDNA Synthesis Kit (5′ and 3′ Adaptors) (Cat# AS-FS-003-02, Arraystar) was used to create cDNA libraries from small RNAs for qPCR detection according to the

(9) RNA â€sequencing reveals the expression profiles of tsRNAs and their potential carcinogenic role in cholangiocarcinomaJournal of Clinical Laboratory AnalysisOctober 1, 2022Yanâ€ke Li, Liâ€rong Yan, Ang Wang, Liâ€yue Jiang, Qian Xu, Benâ€gang WangAS‐FS‐005). cDNA was synthesized with the rtStar™ First‐Strand cDNA Synthesis Kit (3' and 5' adaptor) (Cat#AS‐FS‐003‐02; Arraystar). The primers were designed for Predesigned Human tRNA Primer Sets V2.0. U6 was utilized

(10) A Novel tiRNA-Gly-GCC-1 Promotes Progression of Urothelial Bladder Carcinoma and Directly Targets TLR4Cancers (Basel)September 20, 2022Chuan Qin, Zheng-Hao Chen, Rui Cao, Ming-Jun Shi, Ye Tian(cDNA) was synthesized using the rtStar™ First-Strand cDNA Synthesis Kit (3′ and 5′ adaptors) (Cat# AS-FS-003, Arraystar). qRT-PCR was performed in the QuantStudio™ 5 Real-time PCR System (Applied Biosystems) with

(11) TRFs and tiRNAs sequence in acute rejection for vascularized composite allotransplantationScientific DataSeptember 7, 2022Yuan Fang, Haibo Li, Jingting Chen, Yao Xiong, Xu Li, Binbin Sun, Shengli Li, Jianda Zhou, Shoubao Wangpolymerase chain reaction (RT-qPCR) The rtStar™ First-Strand cDNA Synthesis Kit (3′ and 5′ adaptor) (Cat# AS-FS-003, Arraystar) was used to synthesize cDNA. The QuantStudio™ 5 Real-time PCR System (Applied Biosystems)

(12) Selective cleavage of ncRNA and antiviral activity by RNase2/EDN in THP1-induced macrophagesCellular and Molecular Life SciencesApril 1, 2022Lu Lu, Jiarui Li, Ranlei Wei, Irene Guidi, Luca Cozzuto, Julia Ponomarenko, Guillem Prats-Ejarque, Ester Boixlibraries from small RNAs for qPCR detection using rtStar First-Strand cDNA Synthesis Kit (ArrayStar, AS-FS-003). This method sequentially ligates 3′-adaptor with its 5′-end to the 3′-end of the RNAs, and 5′-adaptor

(13) Differential Expression Profiles and Bioinformatics Analysis of tRNA-Derived Small RNAs in Muscle-Invasive Bladder Cancer in a Chinese PopulationGenes (Basel)March 28, 2022Chuan Qin, Zheng-Hao Chen, Rui Cao, Ming-Jun Shi, Ye Tian(Cat# AS-FS-005, Arraystar, Rockville, MD, USA) and rtStarTM First-Strand cDNA Synthesis Kit (Cat# AS-FS-003, Arraystar), respectively. Quantitative reverse transcription-PCR (qRT-PCR) was performed in a ViiA 7 Real-time PCR System

(14) Differential Expression and Bioinformatics Analysis of tRF/tiRNA in Endometriosis PatientsBioMed Research InternationalMarch 3, 2022Wang-shu Li, Yi-lin Li, Rui Cao, Chun-fang Ha, Shu Sun, Lan Yu, Jian Lithat would interfere with qPCR. The rtStar™ First-Strand cDNA Synthesis Kit (3′ and 5′ adaptor) (Cat# AS-FS-003, Arraystar, USA) was then used to create a cDNA library of small RNAs, which were subjected to 3′-terminal

(15) Expression profiles of tRNA-derived fragments and their potential roles in lung adenocarcinomaAnnals of Translational MedicineFebruary 1, 2022Le-Tian Huang, Meng Cui, Mario Silva, Katsuhiro Okuda, Yoshihisa Shimada, Jia-He Wang, Yi-Bing WangcDNA was synthesized with the rtStar™ First-Strand cDNA Synthesis Kit (3' and 5' adaptor) (catalog no. AS-FS-003, Arraystar, MD, USA). The primers were designed for the tRFs and tiRNAs using Primer 5.0 and synthesized

(16) tRNA-derived fragment TRF365 regulates the metabolism of anterior cruciate ligament cells by targeting IKBKBCell death discoveryJanuary 10, 2022Long D, Xu Y, Mao G, Xin R, Deng Z, Liao H, Li Z, Yang Z, Yu B, Yang Z, et al.miRNA sample pretreatment. The rtStar First-Strand cDNA Synthesis Kit with 3′ and 5′ adaptors (Cat. #AS-FS-003; Arraystar) was used for cDNA synthesis. qRT-PCR was performed using the SYBR Green Pro Taq HS qPCR

(17) tRNA-Derived Fragment tRF-5009A Regulates Autophagy and Degeneration of Cartilage in Osteoarthritis via Targeting mTOROxidative medicine and cellular longevityJanuary 1, 2022Deng Z, Long D, Liu H, Xu Y, Xin R, Liao H, Li Z, Li R, Mao G, Zhang Z, et al.cDNA was synthesized using the rtStar first-strand cDNA synthesis kit with 3′ and 5′ adaptors (Cat. #AS-FS-003; Arraystar). qRT-PCR was performed using an ABI ViiA™ 7 real-time PCR system (ViiA 7Dx; Applied Biosystems,

(18) METTL1‐m ⁷ G‐EGFR/EFEMP1 axis promotes the bladder cancer developmentClinical and Translational MedicineDecember 22, 2021Xiaoling Ying et al.analysis using the rtStra™ First‐Strand cDNA Synthesis Kit (3’ and 5’ adapters) (Arraystar, Cat. #: AS‐FS‐003‐02). qPCR was conducted with the qPCR Mix (Vazyme Biotech, Cat. #: Q711‐02). β‐Actin and U6 were employed

(19) A specific tRNA half, 5’tiRNA-His-GTG, responds to hypoxia via the HIF1α/ANG axis and promotes colorectal cancer progression by regulating LATS2Journal of Experimental & Clinical Cancer ResearchDecember 1, 2021En-Wei Tao, Hao-Lian Wang, Wing Yin Cheng, Qian-Qian Liu, Ying-Xuan Chen, Qin-Yan Gaoefficient cDNA reverse transcription. After sequentially ligating 3′ and 5′ small RNA adapters (#: AS-FS-003, Arraystar), cDNA was synthesized and amplified using Illumina’s proprietary reverse transcription (RT)

(20) Identification of clinical trait-related small RNA biomarkers with weighted gene co-expression network analysis for personalized medicine in endocervical adenocarcinomaAging (Albany NY)September 30, 2021Zhiwen Shi, Xinyu Qu, Chenyan Guo, Lihong Zhang, Chuyue Peng, Zhu Xie, Keqin Hua, Junjun Qiureverse-transcribed into cDNA using rtStar™ First-Strand cDNA Synthesis Kit (3’ and 5’ adaptor) (Cat# AS-FS-003, Arraystar) according to the manufacturer’s protocols. Then, qPCR amplification was performed using

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rtStar™ First-Strand cDNA Synthesis Kit (3’ and 5’ adaptor) from Arraystar, Inc

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Arraystar, Inc

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rtStar™ First-Strand cDNA Synthesis Kit (3’ and 5’ adaptor) from Arraystar, Inc

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