DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag) 14209S from Cell Signaling Technology

Product Specs
ItemDNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number14209S
Quantity1 Kit
Research AreaChromatin Regulation / Nuclear Function
Nucleic Acid TypeChIP DNA
ApplicationsChIP, C&R, C&T

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Histone deacetylase 3 regulates microglial function through histone deacetylationEpigeneticsDecember 31, 2023Laura Meleady, Morgan Towriss, Jennifer Kim, Vince Bacarac, Vivien Dang, Megan E. Rowland, Annie Vogel Cierniaincubation and isolated using DNA Purification Buffers and Spin Columns (Cell Signaling Technology #14209). Yeast spike-in DNA (5ng) was added to each reaction for normalization using Sample Normalization Primer

(2) ONECUT2 Activates Diverse Resistance Drivers of Androgen Receptor-Independent Heterogeneity in Prostate CancerbioRxivOctober 18, 2023Chen Qian, Qian Yang, Mirja Rotinen, Rongrong Huang, Hyoyoung Kim, Brad Gallent, Yiwu Yan, Radu M. Cadaneanu, Baohui Zhang, Salma Kaochar, et al.supernatants were collected. DNA was purified using DNA purification buffers and spin columns (CST, #14209). The CUT & RUN library was generated with the DNA Library Prep Kit for Illumina (CST, #56795) combined

(3) Echinococcus granulosus cyst fluid inhibits inflammatory responses through inducing histone demethylase KDM5B in macrophagesParasites & VectorsSeptember 9, 2023Xiaopeng Wang, Ruolin Lin, Chunxue Fu, Chun Yang, Dan Dong, Xiangwei Wu, Xueling Chen, Lianghai Wang, Jun Houthe protein-DNA crosslinking, DNA was purified using DNA Purification Buffers and Spin Columns (CST #14209). The enrichment of specific DNA sequences following immunoprecipitation was determined by semi-quantitative PCR

(4) CHARGE syndrome-associated CHD7 acts at ISL1-regulated enhancers to modulate second heart field gene expressionCardiovascular ResearchSeptember 5, 2023Athanasia Stathopoulou, Ping Wang, Charlotte Thellier, Robert G Kelly, Deyou Zheng, Peter J Scambler66362, dilution 1/20) as negative control. DNA fragments were purified using DNA Purification kit (CST, 14209). Library preparation was performed with the NEB DNA Ultra II assay with some deviations, as described

(5) Targeting the Clear Cell Sarcoma Oncogenic Driver Fusion Gene EWSR1::ATF1 by HDAC InhibitionCancer Research CommunicationsJuly 3, 2023Hirokazu Mae, Hidetatsu Outani, Yoshinori Imura, Ryota Chijimatsu, Akitomo Inoue, Yuki Kotani, Naohiro Yasuda, Sho Nakai, Takaaki Nakai, Satoshi Takenaka, et al.at 4°C overnight. Antibody-bound DNA was purified using DNA purification buffers and spin columns (14209S; Cell Signaling Technology) and amplified for use as templates in CUT&RUN–qRT-PCR or to construct libraries

(6) TRIM5α recruits HDAC1 to p50 and Sp1 and promotes H3K9 deacetylation at the HIV-1 LTRNature communicationsJune 8, 2023Ran XH, Zhu JW, Ni RZ, Zheng YT, Chen YY, Zheng WH, Mu D.cross-linking with 200 mM NaCl and Proteinase K at 65 °C for 2 h. DNA was then purified by spin columns (14209, Cell Signaling Technology). For qPCR of immunoprecipitated DNA, targeting primers were used to analyze

(7) ZFP92, a KRAB domain zinc finger protein enriched in pancreatic islets, binds to B1/Alu SINE transposable elements and regulates retroelements and genesPLOS GeneticsMay 8, 2023Anna B. Osipovich, Karrie D. Dudek, Linh T. Trinh, Lily H. Kim, Shristi Shrestha, Jean-Philippe Cartailler, Mark A. MagnusonRNAse-containing Stop Buffer (30 min. at 37°C) to release DNA. DNA was further purified using spin columns (14209S, Cell Signaling) and directly used for library prep or qPCR. For sequencing, DNA was processed through

(8) Jarid2 promotes temporal progression of retinal progenitors via repression of Foxp1Cell reportsMarch 28, 2023Zhang J, Roberts JM, Chang F, Schwakopf J, Vetter ML.negative control. DNA fragments were purified on spin columns, eluted with 50 μl buffer (Cell Signaling 14209) and provided to University of Utah High Throughput Genomics Core for library generation. DNA quality

(9) Targeting the chromatin effector Pygo2 promotes cytotoxic T cell responses and overcomes immunotherapy resistance in prostate cancerScience ImmunologyMarch 17, 2023Yini Zhu, Yun Zhao, Jiling Wen, Sheng Liu, Tianhe Huang, Ishita Hatial, Xiaoxia Peng, Hawraa Al Al Janabi, Gang Huang, Jackson Mittlesteadt, et al.Digested DNA was extracted and purified using DNA purification spin columns (Cell Signaling Technology, 14209). The input samples were sonicated for 12 min with a Covaris S220 Ultrasonicator System to obtain a

(10) Receptor activity‐modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axisCell Communication and SignalingDecember 1, 2022Siyuan Yin, Ru Song, Jiaxu Ma, Chunyan Liu, Zhenjie Wu, Guoqi Cao, Jian Liu, Guang Zhang, Huayu Zhang, Rui Sun, et al.and release steps. The extracted DNA was purified using DNA purification buffers and spin columns (#14209; CST). Finally, the DNA products were quantified by qPCR and DNA agarose gel electrophoresis, and the

(11) RAD18 opposes transcription-associated genome instability through FANCD2 recruitmentPLoS geneticsDecember 1, 2022Wells JP, Chang EY, Dinatto L, White J, Ryall S, Stirling PC.proteinase K (CST#10012) overnight at 65°C. DNA samples were purified with Cell Signaling spin columns (#14209S). DNA concentration was measured using NanoDrop One/Onec. DNA samples were used for DRIP analysis, where

(12) HNRNPU promotes the progression of triple-negative breast cancer via RNA transcription and alternative splicing mechanismsCell Death DisNovember 8, 2022Bo-Yue Han, Zhebin Liu, Xin Hu, Hong Lingwas purified with SimpleChIP® DNA Purification Buffers and Spin Columns (Cell Signaling Technology, #14209 S). All the detailed steps were performed according to the instructions of kit instructions. The DNA

(13) Osteocytes directly regulate osteolysis via MYD88 signaling in bacterial bone infectionNature communicationsNovember 4, 2022Yoshimoto T, Kittaka M, Doan AAP, Urata R, Prideaux M, Rojas RE, Harding CV, Henry Boom W, Bonewald LF, Greenfield EM, et al.supernatants were collected. DNA was purified using the DNA purification buffers and spin columns (#14209, CST). qPCR was conducted using primer sets in the Supplementary Table 3 to quantitate the amount of

(14) HSF1 is a driver of leukemia stem cell self-renewal in acute myeloid leukemiaNature communicationsOctober 16, 2022Dong Q, Xiu Y, Wang Y, Hodgson C, Borcherding N, Jordan C, Buchanan J, Taylor E, Wagner B, Leidinger M, et al.chromatin fragments were purified by DNA Purification Buffers and Spin Columns (Cell Signaling Technology, #14209). Libraries were generated using the SMARTer ThruPLEX DNA-seq Kit (TAKARA) and sequenced on the Illumina’s

(15) PHF20 is crucial for epigenetic control of starvation-induced autophagy through enhancer activationNucleic Acids ResearchAugust 12, 2022Se Won Park, Jaehoon Kim, Sungryong Oh, Jeongyoon Lee, Joowon Cha, Hyun Sik Lee, Keun Il Kim, Daechan Park, Sung Hee Baektarget protein is attached. DNA extract was purified with DNA Purification Buffers and Spin Columns (#14209, Cell signaling) and subjected to qRT-PCR. The primers used for qRT-PCR are listed in Supplementary

(16) Integrative analysis and prediction of human R-loop binding proteinsG3 Genes|Genomes|GeneticsJuly 29, 2022Arun Kumar, Louis-Alexandre Fournier, Peter C Stirlingproteinase K (CST#10012) overnight at 65°C. DNA samples were purified with Cell Signaling spin columns (#14209S). DNA concentration was measured using NanoDrop One/Onec. DNA samples were used for DRIP analysis, where

(17) Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosisMolecular therapy. Nucleic acidsDecember 3, 2021Zeng N, Huang YQ, Yan YM, Hu ZQ, Zhang Z, Feng JX, Guo JS, Zhu JN, Fu YH, Wang XP, et al.complex was washed, and DNA was extracted and purified by DNA Purification Buffers and Spin Columns (CST, 14209). ChIP DNA was analyzed by real-time PCR using specific primers, and the data were normalized by input

(18) Effects of Dicyclohexyl Phthalate Exposure on PXR Activation and Lipid Homeostasis in MiceEnvironmental Health PerspectivesDecember 1, 2021Yipeng Sui, Zhaojie Meng, Jianzhong Chen, Jingwei Liu, Rebecca Hernandez, Miko B. Gonzales, Taesik Gwag, Andrew J. Morris, Changcheng Zhouat 65°C for 3 h. The precipitated genomic DNA was purified by using a spin column purification kit (14209; Cell Signaling Technology). qPCR analysis was then performed with the primers targeting the PXR response

(19) RETRACTED ARTICLE: AKT3-mediated IWS1 phosphorylation promotes the proliferation of EGFR-mutant lung adenocarcinomas through cell cycle-regulated U2AF2 RNA splicingNature CommunicationsDecember 1, 2021Georgios I. Laliotis et al.proteinase K, it was extracted with DNA Purification Buffers and Spin Columns (Cell SIgnaling, Cat. No 14209). The immunoprecipitated DNA of the target loci, was then amplified by quantitative PCR, using the sets

(20) RETRACTED ARTICLE: Phosphor-IWS1-dependent U2AF2 splicing regulates trafficking of CAR-E-positive intronless gene mRNAs and sensitivity to viral infectionCommunications BiologyDecember 1, 2021Georgios I. Laliotis et al.were extracted with DNA Purification Buffers and Spin Columns (Cell Signaling Technologies, Cat. No 14209). Real-time PCR using different sets of primers (Supplementary Table 2) to amplify the ISRE genomic

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DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag) from Cell Signaling Technology

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DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag) from Cell Signaling Technology

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