Control CRISPR/Cas9 Plasmid from Santa Cruz Biotechnology, Inc.

Product Specs
ItemControl CRISPR/Cas9 Plasmid
CompanySanta Cruz Biotechnology, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numbersc-418922
Quantity20 µg
Nucleic Acid TypeCRISPR Negative Controls
Vector Type (Standardized)Plasmid
TypePlasmid

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Human RTEL1 Interacts with KPNB1 (Importin β) and NUP153 and Connects Nuclear Import to Nuclear Envelope Stability in S-PhaseCellsDecember 8, 2023Michael Schertzer, Laurent Jullien, André L. Pinto, Rodrigo T. Calado, Patrick Revy, Arturo Londoño-VallejoFigure 1. RTEL1 CRISPR plasmid pools and control (SCR) plasmids were from Santa Cruz (sc-403196A1, and sc-418922, respectively). All sequencing was performed in-house at the Institut Curie Sequencing center using

(2) Enhancement of Radiation Sensitivity by Cathepsin L Suppression in Colon Carcinoma CellsInt J Mol SciDecember 4, 2023Ramadan F. Abdelaziz, Ahmed M. Hussein, Mohamed H. Kotob, Christina Weiss, Krzysztof Chelminski, Tamara Stojanovic, Christian R. Studenik, Mohammed AufyL plasmid (Santa Cruz, sc-419880) and the other with the corresponding control plasmid (Santa Cruz, sc-418922). The transfection process was meticulously executed using the X-tremeGENE™ HP DNA transfection reagent

(3) 3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complexAging CellNovember 13, 2023Zer Vue, Edgar Garza‐Lopez, Kit Neikirk, Prasanna Katti, Larry Vang, Heather Beasley, Jianqiang Shao, Andrea G. Marshall, Amber Crabtree, Alexandria C. Murphy, et al.myotubes were incubated with CRISPR/Cas9 mixtures to produce the following knockouts—control CRISPR/Cas9 (sc‐418922), Chchd6 (Mic25) CRISPR (sc‐425817), Chchd3 (Mic19) CRISPR (sc‐425804), and Mitofilin (Mic60) CRISPR

(4) The Significance of Cathepsin B in Mediating Radiation Resistance in Colon Carcinoma Cell Line (Caco-2)International Journal of Molecular SciencesNovember 9, 2023Ramadan F. Abdelaziz, Ahmed M. Hussein, Mohamed H. Kotob, Christina Weiss, Krzysztof Chelminski, Christian R. Studenik, Mohammed AufyCRISPR/Cas9 plasmid targeting cath B (Santa Cruz sc-400360) or the corresponding control plasmid (Santa Cruz sc-418922). The transfection procedure was conducted using the X-tremeGENE ™ HP DNA transfection reagent sourced

(5) SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9CellOctober 26, 2023Nora Schmidt, Sabina Ganskih, Yuanjie Wei, Alexander Gabel, Sebastian Zielinski, Hasmik Keshishian, Caleb A. Lareau, Liv Zimmermann, Jana Makroczyova, Cadence Pearce, et al.paper,N/A pLEX-SND1-FLAG,This paper,N/A pLEX-NSP9-V5,This paper,N/A CRISPR/Cas9 control plasmid,Santa Cruz Biotechnology,sc-418922 SND1 CRISPR/Cas9 KO plasmids,Santa Cruz Biotechnology,sc-403949 ,, Software and algorithms,Software

(6) NR1D1 Stimulates Antitumor Immune Responses in Breast Cancer by Activating cGAS-STING SignalingCancer ResearchSeptember 15, 2023Na-Lee Ka, Mi Kyung Park, Seung-Su Kim, Yoon Jeon, Sewon Hwang, Sun Mi Kim, Ga Young Lim, Ho Lee, Mi-Ock LeeNR1D1 CRISPR/Cas9 KO plasmid (sc-401211 or sc-432177, Santa Cruz Biotechnology) or a control plasmid (sc-418922) using Lipofectamine 2000 (Thermo Fisher Scientific). GFP-positive cells were sorted by FACS Aria (BD

(7) Etiopathogenic role of ERK5 signaling in sarcoma: prognostic and therapeutic implicationsExperimental & Molecular MedicineJune 19, 2023Adrián Sánchez-Fdez, Sofía Matilla-Almazán, Sofía Del Carmen, Mar Abad, Elena Arconada-Luque, Jaime Jiménez-Suárez, Luis Miguel Chinchilla-Tábora, Mª José Ruíz-Hidalgo, Ricardo Sánchez-Prieto, Atanasio Pandiella, et al.sc-400891-HDR for ERK5 and sc-401688 and sc-401688-HDR for MEK5) and a control CRISPR/Cas9 plasmid (reference sc-418922) from Santa Cruz Biotechnology. Cell proliferation assays were carried out in 24- or 6-well plates.

(8) Functional glycoproteomics by integrated network assembly and partitioningbioRxiv : the preprint server for biologyJune 14, 2023Griffin ME, Thompson JW, Xiao Y, Sweredoski MJ, Aksenfeld RB, Jensen EH, Koldobskaya Y, Schacht AL, Kim TD, Choudhry P, et al.BAP1 CRISPR/Cas9 KO Plasmid (h) (sc-400232), BAP1 HDR Plasmid (h) (sc-400232-HDR), and Control CRISPR/Cas9 Plasmid (sc-418922) were purchased from Santa Cruz Biotechnology (Dallas, TX) and prepared per the manufacturer’s instructions.

(9) SIRT6-PAI-1 axis is a promising therapeutic target in aging-related bone metabolic disruptionScientific reportsMay 17, 2023Aobulikasimu A, Liu T, Piao J, Sato S, Ochi H, Okawa A, Tsuji K, Asou Y.(catalog number: sc-424467-KO-2), HDR (catalog number: sc-424467-HDR-2), and control (catalog number: sc-418922) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Sirt6 knockout was performed according

(10) Interplay between APP and glypican-1 (GPC1) processing and α-synuclein (SYN) aggregation in undifferentiated and differentiated human neural progenitor cells (NPC)GlycobiologyMay 17, 2023Fang Cheng, Lars-Åke Fransson, Katrin Maninonspecific CRISPR/Cas9 control plasmid (not targeting any known gene; Control CRISPR/Cas9 Plasmid, sc-418922; Santa Cruz Biotechnology) according to the manufacturer’s instructions. For transfection, 10 μL of

(11) Fragile X Messenger Ribonucleoprotein 1 (FMR1), a novel inhibitor of osteoblast/osteocyte differentiation, regulates bone formation, mass, and strength in young and aged male and female miceBone ResearchMay 17, 2023Padmini Deosthale, Julián Balanta-Melo, Amy Creecy, Chongshan Liu, Alejandro Marcial, Laura Morales, Julita Cridlin, Sylvia Robertson, Chiebuka Okpara, David J. Sanchez, et al.triplicates. After 24 h-culture, cells were exposed to a mix of 1 μg·μL−1 control (scramble) plasmid (cat# sc-418922; Santa Cruz Biotechnology, Inc) or FMR1 CRISPR/Cas9 plasmid (cat# sc-420392; Santa Cruz Biotechnology, Inc

(12) The Oncosuppressive Properties of KCTD1: Its Role in Cell Growth and MobilityBiologyMarch 21, 2023Smaldone G, Pecoraro G, Pane K, Franzese M, Ruggiero A, Vitagliano L, Salvatore M.obtain SW480 control cells (SW480ctrl), SW480 cells were transfected with a CRISPR/CAS9 control plasmid (sc-418922, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) plus pReceiver-M94 plasmid (GeneCopoeia, Inc., Rockville,

(13) CCL5/CCR5-mediated peripheral inflammation exacerbates blood‒brain barrier disruption after intracerebral hemorrhage in miceJournal of translational medicineMarch 14, 2023Lin J, Xu Y, Guo P, Chen YJ, Zhou J, Xia M, Tan B, Liu X, Feng H, Chen Y.μl/mouse; sc-421200; Santa Cruz Biotechnology, Shanghai, China) or control CRISPR plasmid (2 μl/mouse; sc-418922; Santa Cruz Biotechnology, Shanghai, China) via intracerebroventricular (i.c.v.) injection 25 h before

(14) An anti-mesothelin targeting antibody drug conjugate induces pyroptosis and ignites antitumor immunity in mouse models of cancerJournal for ImmunoTherapy of CancerMarch 6, 2023Nicole L Wittwer, Alexander H Staudacher, Vasilios Liapis, Pina Cardarelli, Harriet Warren, Michael P BrownCRISPR/Cas9 KO plasmid (sc-424934), mouse DFNA5 HDR Plasmid (sc-424934-HDR) and Control CRISPR/Cas9 plasmid (sc-418922) were purchased from Santa Cruz. CRISPR plasmid and HDR plasmid were cotransfected into EMT6 cells using

(15) mTORC1 upregulates B7-H3/CD276 to inhibit antitumor T cells and drive tumor immune evasionNature communicationsMarch 3, 2023Liu HJ, Du H, Khabibullin D, Zarei M, Wei K, Freeman GJ, Kwiatkowski DJ, Henske EP.knockout expression vector (Santa Cruz, Cat#sc-430440) or control CRISPR/Cas9 plasmid (Santa Cruz, Cat#sc-418922) was transfected into Tsc2-deficient 105 K cells. 48 hr after transfection, the transfected cell population

(16) Novel scFv against Notch Ligand JAG1 Suitable for Development of Cell Therapies toward JAG1-Positive TumorsBiomoleculesMarch 2, 2023Silva G, Rodrigues AF, Ferreira S, Matos C, Eleutério RP, Marques G, Kucheryava K, Lemos AR, Sousa PMF, Castro R, et al.HepG2 cells were transfected with JAG1 CRISPRCas9 KO vector-sc-400208-KO-2 or control CRISPRCas9 KO vector-sc-418922 (Santa Cruz Biotechnology, Dallas, TX, USA), separately, both expressing green fluorescent protein (GFP)

(17) IL-32 is induced by activation of toll-like receptors in multiple myeloma cellsFrontiers in ImmunologyFebruary 16, 2023Kristin Roseth Aass, Synne Stokke Tryggestad, Robin Mjelle, Martin H. Kastnes, Tonje Marie Vikene Nedal, Kristine Misund, Therese Standalcells were electroporated with TLR9 (#sc-400600) and TLR4 (sc-400068-KO-2) CRISPR/Cas9 KO and control (#sc-418922) plasmids from Santa Cruz Biotechnology, all containing green fluorescent protein (GFP) for selection.

(18) Reduced SPAG17 Expression in Systemic Sclerosis Triggers Myofibroblast Transition and Drives FibrosisJournal of Investigative DermatologyFebruary 1, 2023Paulene Sapao, Elisha D.O. Roberson, Bo Shi, Shervin Assassi, Brian Skaug, Fred Lee, Alexandra Naba, Bethany E Perez Perez White, Carlos Córdova-Fletes, Pei-Suen Tsou, et al.manufacturer’s recommendations and transfected with CRIPR/Cas9 vectors (Santa Cruz sc410482, Santa Cruz sc418922 or Lenti-U6-SPAG17-EPCG-VSVG, Figure S16A) following standar methods. Detailed procedures are provided

(19) Metabolomic fingerprinting of renal disease progression in Bardet-Biedl syndrome reveals mitochondrial dysfunction in kidney tubular cellsiScienceNovember 18, 2022Marchese E, Caterino M, Viggiano D, Cevenini A, Tolone S, Docimo L, Di Iorio V, Del Vecchio Blanco F, Fedele R, Simonelli F, et al.,Santa Cruz,Sc-428052 Bbs10 HDR plasmid (m),Santa Cruz,Sc-428052-HDR Control CRISPR/Cas9 Plasmid,Santa Cruz,sc-418922 "BBS10 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag",Sino Biological,HG29785-NF ,, Software and

(20) An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memoryNature cell biologyOctober 1, 2022Lanna A, Vaz B, D'Ambra C, Valvo S, Vuotto C, Chiurchiù V, Devine O, Sanchez M, Borsellino G, Akbar AN, et al.primary human CD3+ CD4+ CD27+ CD28+ T cells were transfected with 3mg of control CRISPR/Cas9 Plasmid (sc-418922, Santa Cruz Biotechnology). Knock-out efficiency was confirmed by qPCR (Hs00972656_m1; TaqMan). Transcript

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Control CRISPR/Cas9 Plasmid from Santa Cruz Biotechnology, Inc.

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Control CRISPR/Cas9 Plasmid from Santa Cruz Biotechnology, Inc.

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