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Molecular Biology
DNA
Lambda DNA
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Lambda DNA
Lambda DNA
Lambda DNA from Promega
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Citations:
(18)
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Lambda DNA
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Description
Generates molecular weight size markers used in gel analysis of nucleic acids; nucleotide sequence has been determined
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Lambda DNA
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
D1501
Quantity
250microg
Nucleic Acid Type
Lambda DNA
Species
Bacteriophage
Size Range
48.5 Kbp
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (18)
(1)
Chronic graft-versus-host disease is characterized by high levels and distinctive tissue-of-origin patterns of cell-free DNA
iScience
November 17, 2023
Yifan Pang, Temesgen E. Andargie, Moon Kyoo Jang, Hyesik Kong, Woojin Park, Thomas Hill, Neelam Redekar, Yi-Ping Fu, Desai A. Parth, Noa G. Holtzman,
et al.
cycles),Illumina,20028313
Quant-iT PicoGreen dsDNA assay kit
,
Thermo Fisher Scientific
,
P11496
Lambda DNA
,
Promega
,
D1501
iTaq Universal SYBR Green Supermix
,
Bio-Rad
,
1725122
Primers for Alu115/Alu247,Customized (Available
(2)
Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particles
BMC Biology
December 1, 2021
Marc D. Hein, Prerna Arora, Pavel Marichal-Gallardo, Michael Winkler, Yvonne Genzel, Stefan Pöhlmann, Klaus Schughart, Sascha Y. Kupke, Udo Reichl
assay
(#
P7581
;
Life Technologies
GmbH; Darmstadt, Germany). The standard curve was made with λ DNA (#
D1501
;
Promega
; Madison, USA) in the range of 4–250 ng/mL and had a
limit of detection
of 1.6 ng/mL. The RSD
(3)
Cell culture–based production of defective interfering influenza A virus particles in perfusion mode using an alternating tangential flow filtration system
Applied Microbiology and Biotechnology
October 1, 2021
Marc D. Hein, Anshika Chawla, Maurizio Cattaneo, Sascha Y. Kupke, Yvonne Genzel, Udo Reichl
previously (Opitz et al. 2007). The standard
calibration
curve was made using
lambda DNA
(
Promega
,
D1501
). Calculations The total amount of produced DI244 particles for each cultivation was calculated using
(4)
DoMY-Seq: A yeast two-hybrid–based technique for precision mapping of protein–protein interaction motifs
Journal of Biological Chemistry
January 1, 2021
Pau Castel, Ann Holtz-Morris, Yongwon Kwon, Bernhard P. Suter, Frank McCormick
(5)
A rapid protocol for generating arthropod DNA barcodes suitable for use with undergraduate students
Journal of Biological Education
October 19, 2020
Grant R. Brown, Claire Dagen, Ben Reilly O’Donnell, Jeff A. Graves
experience each tube of mytaq will yield ca. 30 aliquots of 40µl.
Lambda DNA
(pack size 250ug) Item code:
D1501
PROMEGA
£61.20 Oligo's X 4 CHR-2 and LCO1490 sequences INTEGRATED DNA TECHNOLOGIES INC £11.02
Proteinase k
(6)
Pluripotent stem cells with low differentiation potential contain incompletely reprogrammed DNA replication
Journal of Cell Biology
September 7, 2020
Theodore Paniza et al.
(
69506
). One microgram of genomic DNA was spiked with 5 ng/ul of unmethylated cl857 Sam7
Lambda DNA
(
D1501
;
Promega
) to later compute non-conversion rate. The DNA was fragmented to 150–200 bp using a Covaris
(7)
Lineage and Parent-of-Origin Effects in DNA Methylation of Honey Bees (Apis mellifera) Revealed by Reciprocal Crosses and Whole-Genome Bisulfite Sequencing
Genome Biology and Evolution
August 1, 2020
Xin Wu, David A Galbraith, Paramita Chatterjee, Hyeonsoo Jeong, Christina M Grozinger, Soojin V Yi
overhangs and methylated adapters. We added 10 ng of unmethylated
lambda phage
DNA (
Promega
, Cat. No.
D1501
) to the input DNA of each library as a control for bisulfite conversion rates. Bisulfite treatment of
(8)
Distinct epigenomic and transcriptomic modifications associated with Wolbachia-mediated asexuality
PLOS Pathogens
March 18, 2020
Xin Wu, Amelia R. I. Lindsey, Paramita Chatterjee, John H. Werren, Richard Stouthamer, Soojin V. Yi
64˚C. As a control for bisulfite conversion, 10ng of unmethylated
lambda phage
DNA (
Promega
, Cat. No.
D1501
) was added to the 1μg of input DNA. Bisulfite-treated DNA fragments remain single-stranded as they are
(9)
Cell type-specific epigenetic links to schizophrenia risk in the brain
Genome Biology
December 1, 2019
Isabel Mendizabal et al.
and data processing As a control for bisulfite conversion, 10 ng of unmethylated
lambda phage
DNA (#
D1501
,
Promega
) was added to the 1 μg of input DNA. Libraries were made with an in-house Illumina sequencer-compatible
(10)
Joint profiling of DNA methylation and chromatin architecture in single cells
Nature Methods
October 1, 2019
Guoqiang Li, Yaping Liu, Yanxiao Zhang, Naoki Kubo, Miao Yu, Rongxin Fang, Manolis Kellis, Bing Ren
bisulfite conversion with EZ DNA Methylation-Gold™ Kit (Zymo, D5005).
Unmethylated lambda DNA
(
Promega
,
D1501
) was sonicated and ligated with the same adapter for Methyl-HiC sample and then was spiked in at 0.5%
(11)
Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified
Ganoderma lucidum
strain QRS 5120 using response surface methodology
AIMS Microbiology
January 22, 2019
Sugenendran Supramani, 1 Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia, Rahayu Ahmad, Zul Ilham, Mohamad Suffian Mohamad Annuar, Anita Klaus, Wan Abd Al Qadr Imad Wan-Mohtar, 2 Halal Action Laboratory, Kolej Permata Insan, University Sains Islam Malaysia, Bandar Baru Nilai, 71800, Nilai, Negeri Sembilan, Malaysia, 3 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia, 4 Institute for Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia
triphosphates (dNTPs, 200 μM each) (
Promega
no.
U1511
, Madison, OH, USA), 0.5 U DNA polymerase (
Promega
no.
D1501
, Madison, OH, USA), supplied
PCR buffer
(
ThermoFisher
no.
14966123
, Platinum II Green
PCR Buffer
) and
(12)
Global DNA methylation remodeling during direct reprogramming of fibroblasts to neurons
eLife
January 15, 2019
Luo C, Lee QY, Wapinski O, Castanon R, Nery JR, Mall M, Kareta MS, Cullen SM, Goodell MA, Chang HY,
et al
.
were prepared as previously described (Urich et al., 2015), except regular
lambda DNA
(
Promega
cat. #
D1501
) isolated from dcm +
E.Coli
was spiked into samples as the control for non-conversion rate. MethylC-seq
(13)
Environmental enrichment increases transcriptional and epigenetic differentiation between mouse dorsal and ventral dentate gyrus
Nature Communications
December 1, 2018
Tie-Yuan Zhang et al.
in-house using the Wisegene kit as described in Yu et al.22. Three spike-in control DNAs,
lambda DNA
(Cat#
D1501
,
Promega
), 5 mC (Cat# S001, Wisegene) and 5hmC (Cat# S002, Wisegene) were added to each sample (2.5
(14)
Purification of influenza virus-like particles using sulfated cellulose membrane adsorbers
Journal of Chemical Technology & Biotechnology
July 1, 2018
Sofia B Carvalho, A Raquel Fortuna, Michael W Wolff, Cristina Peixoto, Paula M Alves, Udo Reichl, Manuel JT Carrondo
Quant‐iT™ Picogreen® dsDNA reagent
(
P7581
,
Molecular Probes
, USA). The standard curve was prepared with λ‐DNA (#
D1501
,
Promega
GmbH, Germany). The
assay
was carried out in a black 96‐well microplate, flat transparent (
3915
,
(15)
Optimization of cell culture-derived influenza A virus particles purification using sulfated cellulose membrane adsorbers
Engineering in Life Sciences
January 1, 2018
Ana Raquel Fortuna, Florian Taft, Louis Villain, Michael W. Wolff, Udo Reichl
The samples were dialyzed as described in Section 2.6.2. The
calibration
standard was prepared with λ-DNA (#
D1501
,
Promega
GmbH, Mannheim, Germany) in the same
dialysis
buffer, in the range of 4 to 1000 ngDNA mL−1
(16)
MethylC-seq library preparation for base-resolution whole-genome bisulfite sequencing
Nature Protocols
March 1, 2015
Mark A Urich, Joseph R Nery, Ryan Lister, Robert J Schmitz, Joseph R Ecker
Qiagen plant DNeasy and the Qiagen blood and tissue kits Unmethylated λ
phage
DNA (
Promega
, cat. no.
D1501
) Agencourt AMPure XP (
Beckman Coulter
, cat. no.
A63880
) Ethyl alcohol (200 proof, for molecular
(17)
DNA target sequence identification mechanism for dimer-active protein complexes
Nucleic Acids Research
February 1, 2013
Markita P. Landry, Xueqing Zou, Lei Wang, Wai Mun Huang, Klaus Schulten, Yann R. Chemla
was synthesized by filling in the cos ends of λ-DNA with biotinylated dNTPs. Briefly, 1 µg of λ-DNA (
D1501
,
Promega
, Madison, WI, USA) was incubated at room temperature for 10 min with 1 U of
DNA Polymerase I Large Klenow Fragment
(18)
Viral Quantitative Capillary Electrophoresis for Counting and Quality Control of RNA Viruses
Analytical Chemistry
November 6, 2012
Afnan Azizi, Gleb G. Mironov, Darija Muharemagic, Mohamed Wehbe, John C. Bell, Maxim V. Berezovski
Rockland Immunochemicals
, USA); YOYO-1 dye (cat. no.
Y3601
,
Invitrogen
, USA);
lambda DNA
standard (cat. no.
D1501
,
Promega
, USA); RiboGreen and
Escherichia coli
rRNA (16S and 23S) standard from the
Quant-IT RiboGreen RNA Assay Kit
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Lambda DNA from Promega
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