Fig 1: Effect of PRMT7 expression on PTEN and downstream PI3K/AKT signaling pathway. Western blot was used to detect the effect of low/high PRMT7 expression on the expression of PTEN and related proteins in the downstream PI3K/AKT signaling pathway. *P < 0.05, **P < 0.01, ***P < 0.001 and ns: no significance.
Fig 2: PRMT7 affects gastric cancer cell proliferation and migration via the PI3K/AKT signaling pathway. A CCK-8 assay was used to detect the effect of LY294002 on the viability of gastric cancer cells. B Colony formation assay was used to detect the effect of LY294002 on the proliferation of gastric cancer cells. C Transwell assay was used to detect the effect of LY294002 on the migration ability of gastric cancer cells. D LY294002 reversed the effect of PRMT7 knockdown on PI3K/AKT signaling. *P < 0.05, **P < 0.01, ***P < 0.001 and ns: no significance.
Fig 3: PRMT7 plays a tumor suppressor role dependent on PTEN. A Effect of PRMT7 overexpression on the viability of PTEN-deficient gastric cancer cells. B Effect of PRMT7 overexpression on the proliferation of PTEN-deficient gastric cancer cells. C Effect of PRMT7 overexpression on the migration of PTEN-deficient gastric cancer cells. D Knockdown of PTEN reversed the effect of PRMT7 upregulation on the expression of PTEN and its downstream signaling pathways and target proteins. *P < 0.05, **P < 0.01, ***P < 0.001 and ns: no significance.
Fig 4: PRMT7 interacts with PTEN and promotes PTEN methylation. A RT-qPCR was used to detect the expression of PTEN mRNA in the transfected cells. B Endogenous PTEN and PRMT7 immunoprecipitation occurred in AGS cells. C Exogenous PTEN and PRMT7 co-immunoprecipitated in AGS cells. D AGS cells transfected with PRMT7 siRNA and overexpression plasmid were subjected to co-immunoprecipitation with PTEN antibody, followed by detection of PTEN methylation level with MMA antibody. *P < 0.05, **P < 0.01, ***P < 0.001 and ns: no significance.
Fig 5: PRMT7 is expressed at low levels in gastric cancer tissues. A Immunohistochemical analyses were performed to observe PRMT7 expression in gastric cancer: a) normal gastric tissue; b) high-medium differentiated gastric cancer tissue; c) poorly differentiated gastric cancer tissue. B Western blot analysis of PRMT7 protein expression levels in 30 paired gastric cancer tissues and adjacent normal mucosal tissues. N: normal mucosa adjacent to cancer; T: tumor tissue. C PRMT7 expression in the GES-1 cell line and various gastric cancer cell lines. ***P < 0.001.
Supplier Page from Sino Biological, Inc. for Human PRMT7 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-His tag