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Products
XbaI
XbaI
Supplier Images
XbaI
XbaI from New England Biolabs
(1)
Write a review
Citations:
(157)
Supplier Page
Supplier Page from
New England Biolabs for
XbaI
Get Pricing
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
XbaI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0145S
Quantity
3000 units
Concentration
20-100 u/µL
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
T/CTAGA
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (157)
(1)
Impaired proliferation and migration of HUVEC and melanoma cells by human anti-FGF2 mAbs derived from a murine hybridoma by guided selection
Bioengineered
December 31, 2023
Carolina Georg Magalhães, Carla Ploeger Mansueli, Tânia Maria Manieri, Wagner Quintilio, Angélica Garbuio, Juan de Jesus Marinho, Jane Zveiter de Moraes, Lilian Rumi Tsuruta, Ana Maria Moro
were double-digested with
SacI
/
XbaI
restriction enzymes (
New England Biolabs
, Catalog No.
R3156S
and
R0145S
), purified using
Wizard SV Gel and PCR Clean-Up System
kit (
Promega
, Catalog No.
A9281
), and ligated
(2)
Dimerization of melanocortin 4 receptor controls puberty onset and body size polymorphism
Front Endocrinol (Lausanne)
November 10, 2023
Ruiqi Liu, Mike Friedrich, Katherina Hemmen, Kerstin Jansen, Mateus C. Adolfi, Manfred Schartl, Katrin G. Heinze
restriction enzymes
HindIII
(
R3104S
,
New England Biolabs
, Ipswich, Massachusetts, United States) and
XbaI
(
R0145S
,
New England Biolabs
), and ligated to backbones pcDNA3-eGFP/mCherry in frame with eGFP/mCherry encoding
(3)
Systematic investigation of allelic regulatory activity of schizophrenia-associated common variants
Cell Genomics
October 11, 2023
Jessica C. McAfee, Sool Lee, Jiseok Lee, Jessica L. Bell, Oleh Krupa, Jessica Davis, Kimberly Insigne, Marielle L. Bond, Nanxiang Zhao, Alan P. Boyle,
et al.
using Zymo Gel DNA Recovery kit. The gel extracted products were then digested with
XbaI
(
NEB
, cat#
R0145S
) and rSAP for 3 h at 37°C, and then for 10 min at 65°C for heat inactivation. The digested products
(4)
Zmym4 is required for early cranial gene expression and craniofacial cartilage formation
Frontiers in Cell and Developmental Biology
October 3, 2023
Karyn Jourdeuil, Karen M. Neilson, Helene Cousin, Andre L. P. Tavares, Himani D. Majumdar, Dominique Alfandari, Sally A. Moody
subcloned into the BamHI (
Thermo Fisher Scientific
Cat#
FD0054
) and
XbaI
(
New England Biolabs
Cat#
R0145S
) sites of pCS2+ (pCS2+zmym4) using the
Clone
EZ PCR cloning kit (GenScript
Gene Synthesis
Cat# L00339).
(5)
The RNA m5C modification in R-loops as an off switch of Alt-NHEJ
Nature Communications
September 30, 2023
Haibo Yang, Emily M. Lachtara, Xiaojuan Ran, Jessica Hopkins, Parasvi S. Patel, Xueping Zhu, Yao Xiao, Laiyee Phoon, Boya Gao, Lee Zou,
et al.
(
New England Biolabs
Cat #
R3104L
),
SspI
(
New England Biolabs
Cat #
R3132L
), and
XbaI
(
New England Biolabs
Cat #
R0145L
) in 1x NEB Buffer 2 (Cat # B7002) supplemented with 1X
BSA
(
New England Biolabs
Cat # B9000) and 2 mM
(6)
An efficient cloning method to expand vector and restriction site compatibility of Golden Gate Assembly
Cell Reports Methods
August 22, 2023
Masato Sorida, Roberto Bonasio
,
Thermo Scientific
,
SM1331
BsaI-HFv2
,
NEB
,
R3733S
BbsI-HF
,
NEB
,
R3539S
AgeI-HF
,
NEB
,
R3552S
BamHI-HF
,
NEB
,
R3136S
EcoRI-HF
,
NEB
,
R3101L
HindIII-HF
,
NEB
,
R3104S
NcoI-HF
,
NEB
,
R3193S
NotI-HF
,
NEB
,
R3189S
SalI-HF
,
NEB
,
R3138S
SpeI-HF
,
NEB
,
R3133S
XbaI
,
NEB
,
R0145S
XhoI
,
NEB
,
R0146S
XmaI
,
NEB
,
R0180S
KpnI-HF
,
NEB
,
R3142S
NdeI
,
NEB
,
R0111S
Smal,
NEB
,
R0141S
BbsI-HF
,
NEB
,
R3539M
T4 DNA Ligase
(7)
Differentiation block in acute myeloid leukemia regulated by intronic sequences of FTO
iScience
August 18, 2023
Francesco Camera, Isabel Romero-Camarero, Bradley H. Revell, Fabio M.R. Amaral, Oliver J. Sinclair, Fabrizio Simeoni, Daniel H. Wiseman, Lovorka Stojic, Tim C.P. Somervaille
Green,
ThermoFisher
,Cat#
4309155
May-Grunwald Giemsa,Sigma,Cat#MG500 Alt-R Cas9,Integrated DNA Technologies,Cat#1081059
XbaI
,
NEB
,Cat#
R0145L
SbfI
,
NEB
,Cat#
R3642L
Age1,
NEB
,Cat#
R3552S
EcoR1-HF,
NEB
,Cat#
R3101L
DpnII
,
NEB
,Cat#
R0543S
NlaIII
,
NEB
,Cat#
R0125S
,, Critical
(8)
Fluorescent PCR-based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in Zebrafish
Bio Protoc
August 5, 2023
Blake Carrington, Raman Sood
plasmid (Addgene, catalog number: 46757)
XbaI
and
CutSmart buffer
(
New England Biolabs
, catalog number:
R0145S
)QIAquick PCR Purification kit (
Qiagen
, catalog number:
28104
)
mMessage mMachine T3 Transcription kit
(9)
Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24
iScience
July 21, 2023
Patel LR, Stratton SA, McLaughlin M, Krause P, Allton K, Rivas AL, Barbosa D, Hart T, Barton MC.
CutSmart Buffer
,
NEB
,B7204
Esp3I
,
Thermo
,
ER0452
EcoRI
,
NEB
,
R0101S
BamHI
,
NEB
,
R0136S
T4 Ligase,
NEB
,
M0202S
XbaI
,
NEB
,
R0145S
HindIII
,
NEB
,
R0104S
Q5 Site Direct
Mutagenesis
Kit,NEB,E0554 Herculase II Polymerase,
Agilent
,
600675
Clontech
(10)
Systematic characterization of regulatory variants of blood pressure genes
Cell Genomics
July 12, 2023
Winona Oliveros, Kate Delfosse, Daniella F. Lato, Katerina Kiriakopulos, Milad Mokhtaridoost, Abdelrahman Said, Brandon J. McMurray, Jared W.L. Browning, Kaia Mattioli, Guoliang Meng,
et al.
,
STEMCELL Technologies
,Cat#
05010
SfiI
,
NEB
,Cat#
R0123L
"Alkaline Phosphatase, Calf Intest",
NEB
,Cat#
M0290L
T4 DNA Ligase
,
NEB
,Cat#
M0202M
XbaI
,
NEB
,Cat#
R0145L
KpnI-HF
,
NEB
,Cat#
R3142L
Q5- High Fidelity DNA polymerase
,
NEB
,Cat#
M0491L
Formamide (Deionized
),
Invitrogen
,Cat#
AM9342
Agencourt
(11)
Large-scale Purification of Type III Toxin-antitoxin Ribonucleoprotein Complex and its Components from
Escherichia coli
for Biophysical Studies
BIO-PROTOCOL
July 5, 2023
Parthasarathy Manikandan, Kavyashree Nadig, Mahavir Singh
Laboratories, catalog number: 21113, CAS number: 57-13-6)
XhoI
(
New England Biolabs
, catalog number:
R0145S
), store at -20 °Cβ-Mercaptoethanol (Sisco Research Laboratories, catalog number: 83759, CAS number:
(12)
ERα-associated translocations underlie oncogene amplifications in breast cancer
Nature
June 29, 2023
Jake June-Koo Lee, Youngsook Lucy Jung, Taek-Chin Cheong, Jose Espejo Valle-Inclan, Chong Chu, Doga C. Gulhan, Viktor Ljungström, Hu Jin, Vinayak V. Viswanadham, Emma V. Watson,
et al.
endogenous SHANK2 and RARA locus,
ligation
reactions were digested with
XbaI
(
New England Biolabs
,
R0145L
) for SHANK2 locus and
EcoRI
(
New England Biolabs
,
R0101L
) for RARA locus, respectively. In the first
(13)
eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content
The EMBO journal
June 15, 2023
Duan H, Zhang S, Zarai Y, Öllinger R, Wu Y, Sun L, Hu C, He Y, Tian G, Rad R,
et al
.
,
New England Biolabs
,Cat #
R0580S
BbSI
,
New England Biolabs
,Cat #
R0539S
XbaI
,
New England Biolabs
,Cat #
R0145S
XhoI
,
New England Biolabs
,Cat #
R0146S
BamHI
,
New England Biolabs
,Cat #
R3136S
T4 Polynucleotide Kinase
,
New England Biolabs
(14)
Regulatory architecture of housekeeping genes is driven by promoter assemblies
Cell reports
May 30, 2023
Dejosez M, Dall'Agnese A, Ramamoorthy M, Platt J, Yin X, Hogan M, Brosh R, Weintraub AS, Hnisz D, Abraham BJ,
et al
.
pTZ2073 to shorten the sequence between the Max and Fntb promoters. pTZ2073 was cut with
XbaI
(
NEB
,
R0145S
) and
NheI
(
NEB
,
R0131L
) and the 5383 bp fragment was religated after
gel electrophoresis
and
purification
(15)
TAR Syndrome-associated Rbm8a deficiency causes hematopoietic defects and attenuates Wnt/PCP signaling
bioRxiv
May 5, 2023
Agnese Kocere, Elena Chiavacci, Kevin Manuel Méndez-Acevedo, Charlotte Soneson, Max S. Hiltabidle, Azhwar Raghunath, Jacalyn S. MacGowan, Jordan A. Shavit, Daniela Panáková, Margot L. K. Williams,
et al.
restriction digest
into Tol2kit vector 396 (pCS2FA:Tol2) (Kwan et al., 2007) cut with
BamHI
and
XbaI
(
R0145S
,
NEB
). Morpholinos were obtained from Gene Tools (Philomath, USA) and prepared as a 1 mM stock solution
(16)
Production and characterization of cell-penetrating recombinant botulinum neurotoxin type A
STAR protocols
March 17, 2023
Wei X, Hu J, Jiang L, Li L, Liu J.
Phanta Max Super-Fidelity DNA Polymerase
(1 unit/μL),
Vazyme
,
P505-d3
XbaI
(20 units/μL),
New England Biolabs
,
R0145L
HindIII
(20 units/μL),
New England Biolabs
,
R0104L
Cutsmart buffer
10×,
New England Biolabs
,B7204V
Quick CIP
(17)
Multimodal CRISPR perturbations of GWAS loci associated with coronary artery disease in vascular endothelial cells
PLOS Genetics
March 16, 2023
Florian Wünnemann, Thierry Fotsing Tadjo, Mélissa Beaudoin, Simon Lalonde, Ken Sin Lo, Benjamin P. Kleinstiver, Guillaume Lettre
the gateway cassette from pLVX-EF1α-attR1-ccdB-attR2-IRES-puro-emGFP digested with
XbaI
(
NEB
, cat no
R0145S
) and ligated with Quick ligase from
NEB
(cat no
M2200
) in pLVX-EF1α-IRES-mCherry (refer as Empty-mCherry
(18)
Prediction and verification of glycosyltransferase activity by bioinformatics analysis and protein engineering
STAR Protocols
March 1, 2023
Dietlind L. Gerloff, Elena I. Ilina, Camille Cialini, Uxue Mata Salcedo, Michel Mittelbronn, Tanja Müller
V717A UDP-glucose,
Sigma-Aldrich
,Cat#
U4625
Ethidium bromide
,Bio-Rad,Cat#1610433EDU Gel loading dye 6×,
NEB
,Cat#
B7024S
XbaI
,
NEB
,Cat #
R0145S
NotI-HF
,
NEB
,Cat#
R3189S
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
(19)
Protocol for investigating the biogenesis of SARS-CoV-2 S pseudoviruses in HEK293T cells transduced to express the virus-specific intrabodies
STAR Protocols
March 1, 2023
Surbhi Dahiya, Sudhakar Singh, Sharvan Sehrawat
FemtoLUCENT plus AP kit
,
G-Biosciences
,Cat#
786-10AP
PEI branched,Sigma,408727-100mL
BamHI
,New England Biolab (
NEB
),
R3136S
XbaI
,
NEB
,
R0145S
NcoI
,
NEB
,
R3193S
NotI
,
NEB
,
R3189S
MG132,Sigma,474787 Ni-NTA probes,ForteBio,18-5101 Clarity Western
(20)
Inhibition of SARS-CoV-2 infection in cellular systems using engineered trimeric receptor-binding domain of spike protein
STAR Protocols
March 1, 2023
Angela Rose Liu, Shrikanth C. Basavarajappa, Nandini Sarkar, Anna Bruchez, Parameswaran Ramakrishnan
Biomedicals,802829
TEMED
,
Bio-Rad
,
161-0801
25% Glutaraldehyde,Sigma,354400 Xho1,
NEB
,
R0146L
Xba1,
NEB
,
R0145L
HindIII
,
NEB
,
R0104L
Agarose,Fisher,BP160-500
Ethidium bromide
,
Sigma
,E8751
Ligafast Rapid DNA Ligation System
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Reviews
Write a Review
(1)
9/11/2015
Good XbaI Restriction Enzyme
J G
This is a good source of the XbaI restriction enzyme. I use this product when doing diagnostic restriction digests or in molecular cloning when I need to cut out an insert.
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XbaI from New England Biolabs
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