pAdVAntage Vector from Promega

Product Specs
ItempAdVAntage Vector
CompanyPromega
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE1711
HostMammalian
Vector NamepAdVAntage™
Quantity20microg
ResistanceAmpicillin
Reporterluciferase

Specifications/Features

Vector Size:
4392 bp

Features:
• Increased Expression: Co-transfection of pAdVAntage™ Vector with luciferase constructs showed an approximately 27-fold increase in luciferase expression in 293 and HeLa cell lines over transfections performed with the construct DNA alone.
• Flexible: Can be used in a variety of cell lines.

Add to Compare List

Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) RTK signalling promotes epithelial columnar cell shape and apical junction maintenance in human lung progenitor cellsDevelopmentJune 1, 2023Shuyu Liu, Dawei Sun, Richard Butler, Emma L. Rawlinspackaging plasmids: pMD2.G (3 μg, Addgene 12259), psPAX2 (6 μg, Addgene 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, ThermoFisher Scientific) according

(2) Genetically engineered pair of cells for serological testing and its application for SARS‐CoV‐2Bioengineering & Translational MedicineMay 1, 2023Marvin A. Ssemadaali, Juan Arredondo, Elise A. Buser, Sherri Newmyer, Harikrishnan Radhakrishnan, Harold S. Javitz, Satya Dandekar, Parijat BhatnagarFédérale de Lausanne, Lausanne, Switzerland) through Addgene. pAdVAntageTM was obtained from Promega (Cat #E1711). PiggyBac Transposase from Reference 26 was used. All plasmid preparation services (chemical synthesis

(3) CRISPR-based large-scale modeling of loss-of-function mutations to investigate mechanisms of stress resistance in cancerSTAR ProtocolsMarch 1, 2023Fabrizio Simeoni, Ioannis Loukas, Thomas Stuart Wilson, Paola ScaffidiAddgene_12260 Plasmid: pMD2.G,Trono Lab,Addgene; Cat#12259; RRID: Addgene_12259 Plasmid: pAdVAntage™ Vector,Promega,Cat#E1711 Plasmid: arrayed lentiviral sgRNA library,Henser-Brownhill et al.3Loukas et al.1,Table S2 from Henser-Brownhill

(4) Regulation of cargo exocytosis by a Reps1-Ralbp1-RalA moduleScience advancesFebruary 22, 2023Wang S, Chen X, Crisman L, Dou X, Winborn CS, Wan C, Puscher H, Yin Q, Kennedy MJ, Shen J.produced by transfecting GeCKO v2 library plasmids into 293T cells along with pAdVAntage (Promega, #E1711), pCMV-VSVG (Addgene, #8454), and psPax2 (Addgene, #12260) as previously described (19, 21). Starting

(5) A fast-growing dengue virus mutant reveals a dual role of STING in response to infectionOpen biologyDecember 1, 2022Ng WC, Kwek SS, Sun B, Yousefi M, Ong EZ, Tan HC, Puschnik AS, Chan KR, Ooi YS, Ooi EE.pMDLg/pRRE (Addgene #12251), pMD2.G (Addgene #12259), pRSV-Rev (Addgene #12253) and pAdVantage (Promega E1711) using transIT-LT1 transfection reagent (Mirus Bio) to produce lentivirus in 293FT cells. In parallel,

(6) SOX9 maintains human foetal lung tip progenitor state by enhancing WNT and RTK signallingThe EMBO journalNovember 2, 2022Sun D, Llora Batlle O, van den Ameele J, Thomas JC, He P, Lim K, Tang W, Xu C, Meyer KB, Teichmann SA, et al.pMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according

(7) Cell-Based Platform for Antigen Testing and Its Application for SARS-CoV-2 InfectionMicrobiology SpectrumAugust 31, 2022Marvin A. Ssemadaali, Sherri Newmyer, Harikrishnan Radhakrishnan, Juan Arredondo, Harold S. Javitz, Satya Dandekar, Parijat BhatnagarLausanne, Switzerland) through Addgene (40), while pAdvantage was obtained from Promega (catalog number E1711). piggyBac transposase sequence was provided by Nancy Craig (Johns Hopkins University School of Medicine).

(8) AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decayMolecular cellAugust 4, 2022Cho H, Abshire ET, Popp MW, Pröschel C, Schwartz JL, Yeo GW, Maquat LE.DNA pCG-GAG/Pol,"Ulm et al., 2007",N/A pCMV-VSV-G,"Stewart et al., 2003",Addgene #8454 pAdvantage,Promega,E1711 pGT GFP(0),"Carette et al., 2011",Nat Biotechnol. 2011 Jun; 29(6):542–546 pGT GFP(+1),"Carette et

(9) Direct reprogramming of adult adipose-derived regenerative cells toward cardiomyocytes using six transcriptional factorsiScienceJuly 15, 2022Shingo Narita et al.pCMV-R8.74 (Addgene, Cat# 22036), 4 μg pCMV-VSV-G (Addgene, Cat# 8454), and 2 μg pAdVAntage (Promega, Cat# E1711) were diluted in 500 μl of Opti-MEM (Gibco, Cat# 31985-062). A quantity of 60 μl of lipofectamine 2000

(10) ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growthFrontiers in molecular biosciencesJanuary 1, 2022Park J, Park GY, Lee J, Park J, Kim S, Kim E, Park SY, Yoon JH, Lee Y.HEK293T cells were co-transfected with gag/pol (14887, Addgene), pVSVg (8454, Addgene), pAdVAntage (E1711, Promega), and either MIGR1-FLAG-CIC-SWT-GFP, MIGR1-FLAG-CIC-SCH1∼5A-GFP, or MIGR1-GFP control retroviral

(11) Prestimulation of CD2 confers resistance to HIV-1 latent infection in blood resting CD4 T cellsiScienceNovember 19, 2021Sijia He et al.proviral DNA,"Englund et al., 1995",N/A pCMV4-BlaM-Vpr,Dr. Warner C. Greene,N/A pAdVAntage,Promega,Cat# E1711 pLifeAct-EGFP,Addgene,58470 pCMV3-CD2-C-GFPSpark,Sino Biological,Cat#HG10982-ACG pCMV3-CXCR4-C-OFPSpark,Sino Biological

(12) RUSC2 and WDR47 oppositely regulate kinesin-1-dependent distribution of ATG9A to the cell peripheryMolecular biology of the cellNovember 1, 2021Guardia CM, Jain A, Mattera R, Friefeld A, Li Y, Bonifacino JS.delivery. Additional plasmids psPAX2 (Addgene #12260), pMD2.G (Addgene #12259), and pAdVantage (Promega, E1711) were used for lentivirus production. Plasmids encoding GFP-RUSC2 mutants were generated by PCR and

(13) A Strategy for Selective Deletion of Autoimmunity-Related T Cells by pMHC-Targeted DeliveryPharmaceuticsOctober 13, 2021Shalom D. Goldberg et al.alpha and beta chains, along with the pAdvantage plasmid to enhance protein expression (Promega Cat# E1711), were mixed with OptiMEM media. After 5 days total of incubation at 37 °C, 120 rpm, 8% CO2, supernatants

(14) A functional genetic toolbox for human tissue-derived organoidseLifeOctober 6, 2021Dawei Sun, Lewis Evans, Francesca Perrone, Vanesa Sokleva, Kyungtae Lim, Saba Rezakhani, Matthias Lutolf, Matthias Zilbauer, Emma L RawlinspMD2.G (3 μg, Addgene plasmid # 12259), psPAX2 (6 μg, Addgene plasmid # 12260) and pAdVAntage (3 μg, E1711, Promega) using Lipofectamine 2000 Transfection Reagent (11668019, Thermo Fisher Scientific) according

(15) Lipid-mediated motor-adaptor sequestration impairs axonal lysosome delivery leading to autophagic stress and dystrophy in Niemann-Pick type CDevelopmental cellMay 17, 2021Roney JC, Li S, Farfel-Becker T, Huang N, Sun T, Xie Y, Cheng XT, Lin MY, Platt FM, Sheng ZH.# 12260 pMD2.G,A gift from Dr. Didier Trono (EPFL),Addgene plasmid # 12259 pAdVAntage,Promega,Cat#E1711 pGEX-KG-D4H*-mCherry,"(Lim et al., 2019)",Addgene plasmid # 134604 Software and Algorithms,Software

(16) Genetic evidence for an inhibitory role of tomosyn in insulin‐stimulated GLUT4 exocytosisTrafficFebruary 22, 2021Shifeng Wang, Yinghui Liu, Lauren Crisman, Chun Wan, Jessica Miller, Haijia Yu, Jingshi Shenselection marker (60). CRISPR plasmids were transfected into 293T cells along with pAdVAntage (Promega, #E1711), pCMV-VSVG (Addgene, #8454), and psPax2 (Addgene, #12260) as previously described (60). The 293T cell

(17) Genetic Screens Identify Host Factors for SARS-CoV-2 and Common Cold CoronavirusesCellJanuary 7, 2021Wang R, Simoneau CR, Kulsuptrakul J, Bouhaddou M, Travisano KA, Hayashi JM, Carlson-Stevermer J, Zengel JR, Richards CM, Fozouni P, et al.2003",Addgene Cat#8455 pCMV-VSV-G,"Stewart et al., 2003",Addgene Cat#8454 pAdVAntage,Promega,Cat#E1711 pLenti-DsRed_IRES_EGFP,"Rousseaux et al., 2016",Addgene Cat#92194 PIK3R4,"Johannessen et al., 2010",Addgene

(18) Comparison of Two Strategies to Generate Antigen-Specific Human Monoclonal Antibodies: Which Method to Choose for Which Purpose?Frontiers in immunologyJanuary 1, 2021Ehlers AM, den Hartog Jager CF, Kardol-Hoefnagel T, Katsburg MMD, Knulst AC, Otten HG.resistance gene for selection), pfuse2ss-hcll, Invivogen, San Diego, California, USpAdVAntage™ Vector, E1711, Promega, Madison, Wisconsin, US Cell Lines Human embryonic kidney cells (HEK293-F (RRID : CVCL_6642),

(19) PBRM1 and the glycosylphosphatidylinositol biosynthetic pathway promote tumor killing mediated by MHC-unrestricted cytotoxic lymphocytesScience advancesNovember 1, 2020Menasche BL, Davis EM, Wang S, Ouyang Y, Li S, Yu H, Shen J.293T cells with a cocktail of plasmids including pGT-GFP0, pGT-GFP1, pGT-GFP2, pAdVAntage (Promega, no. E1711), pGAL, and pCMV-VSVG using TurboFectin 8.0 (OriGene, no. TF81001). The retroviruses were collected

(20) Ribosomal protein RACK1 enhances translation of poliovirus and other viral IRESsVirologyJune 1, 2020Ethan LaFontaine, Clare M. Miller, Natasha Permaul, Elliot T. Martin, Gabriele FuchspCMV-dR8.2 dvpr (gag-pol; Addgene #8455), pCMV-VSV-G (envelope; Addgene #8454), pAdVantage (Promega E1711) and pLenti RACK1-FLAG using Fugene HD (Promega). The lentivirus-containing media was filtered through

Be the First to Write a Review!