Tween 20, Molecular Biology Grade from Promega

Product Specs
ItemTween 20, Molecular Biology Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberH5152
Quantity100ml

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Progenitor cell isolation from mouse epididymal adipose tissue and sequencing library constructionSTAR ProtocolsDecember 1, 2023Qianglin Liu, Chaoyang Li, Yuxia Li, Leshan Wang, Xujia Zhang, Buhao Deng, Peidong Gao, Mohammad Shiri, Fozi Alkaifi, Junxing Zhao, et al.T2194-100ML Digitonin,Sigma,Cat# 300410 Calcium chloride solution,Sigma,Cat# 21115-100ML Tween 20,Promega,Cat# H5152 Tris-HCl (pH = 8.0),VWR,Cat# 97062 TE buffer,Sigma,Cat# 93283-100ML Magnesium chloride,Sigma,Cat#

(2) A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organsCell reportsJune 27, 2023van der Valk WH, van Beelen ESA, Steinhart MR, Nist-Lund C, Osorio D, de Groot JCMJ, Sun L, van Benthem PPG, Koehler KR, Locher H.Microsystems). For immunostaining, sections were rinsed in washing buffer consisting of 0.05% Tween-20 (H5152, Promega) and incubated for 30 min with blocking solution consisting of 5% bovine serum albumin (BSA;

(3) Tcf21 marks visceral adipose mesenchymal progenitors and functions as a rate-limiting factor during visceral adipose tissue developmentCell reportsMarch 28, 2023Liu Q, Li C, Deng B, Gao P, Wang L, Li Y, Shiri M, Alkaifi F, Zhao J, Stephens JM, et al.,Beckman Coulter,Cat# A63881 Sodium Chloride,Sigma,Cat# 59222C Digitonin,Sigma,Cat# 300410 Tween 20,Promega,Cat# H5152 Tris,VWR,Cat# 97062 Magnesium Chloride,Sigma,Cat# M1028 DMF,Sigma,Cat# D4551 HCS LipidTOX™ Deep

(4) FRET-FISH probes chromatin compaction at individual genomic loci in single cellsNature communicationsNovember 5, 2022Mota A, Berezicki S, Wernersson E, Harbers L, Li-Wang X, Gradin K, Peuckert C, Crosetto N, Bienko M.The next day, we washed the coverslips twice, 5 min each at 65 °C in 0.2% Tween (Promega, cat. no. H5152)/0.2× SSC pre-warmed to 65 °C inside a water bath, followed by a brief wash in 0.2% Tween/4× SSC at

(5) Restoration of Cathepsin D Level via L-Serine Attenuates PPA-Induced Lysosomal Dysfunction in Neuronal CellsInternational Journal of Molecular SciencesSeptember 13, 2022Hyunbum Jeon, Yeo Jin Kim, Su-Kyeong Hwang, Jinsoo Seo, Ji Young Munmembranes were blocked using 3% BSA (#9048-4-8, GENEray Biotechnology, Shanghai, China) and 0.1% Tween 20 (H5152, Promega, Madison, WI, USA) in TBST buffer (#CBTB-9110, CHEMBIO, Seoul, Korea) for 1 h and washed with

(6) Corticosterone antagonist or TrkB agonist attenuates schizophrenia-like behavior in a mouse model combining Bdnf-e6 deficiency and developmental stressiScienceJuly 15, 2022Chen Y, Li S, Zhang T, Yang F, Lu B.M8046 Tween-80,Sigma-Aldrich,Cat# P1754 MK801,Sigma-Aldrich,Cat# M107-5MG Tween-20,Promega,Cat# H5152 Normal Donkey Serum,Jackson ImmunoResearch Labs,Cat# 017-000-121 Triton™ X-100,Sigma-Aldrich,Cat#

(7) Pirfenidone Sensitizes NCI-H460 Non-Small Cell Lung Cancer Cells to Paclitaxel and to a Combination of Paclitaxel with CarboplatinInternational Journal of Molecular SciencesMarch 26, 2022Helena Branco, Júlio Oliveira, Catarina Antunes, Lúcio L. Santos, Maria Helena Vasconcelos, Cristina P. R. Xavier258148) for 20 min and blockage was performed with a PBS with 0.5% Tween (Promega, Madison, WI, USA; H5152) and 0.05% Bovine Serum Albumin (BSA; Merck Life Science, Darmstadt, Germany; A7906) solution. Then,

(8) Reciprocal Regulation of Mitofusin 2-Mediated Mitophagy and Mitochondrial Fusion by Different PINK1 Phosphorylation EventsFrontiers in cell and developmental biologyJanuary 1, 2022Li J, Dang X, Franco A, Dorn GW 2nd.1706404) in 1x phosphate-buffered saline, 0.05% Tween-20 (PBS, Sigma, P5368; Tween-20, Promega, and H5152) at room temperature for 30 min, incubated with primary antibodies diluted in blocking buffer (room

(9) Real-time monitoring of deformed wing virus-infected bee foraging behavior following histone deacetylase inhibitor treatmentiScienceOctober 22, 2021Cheng-Kang Tang, Yu-Hsien Lin, Joe-Air Jiang, Yun-Heng Lu, Chih-Hsuan Tsai, Yu-Chun Lin, Yun-Ru Chen, Carol-P Wu, Yueh-Lung WuBrands (Far East) Limited, Taiwan Branch (H.K.)",A-112430309-00000-0 0.05% Tween-20,Promega Corporation,H5152 Acetyl-H3,Merck Millipore,Cat# 06-599; RRID:AB_2115283 Acetyl-H4,Merck Millipore,Cat# 06-866; RRID:AB_310270 Calpain-1,ABcam,Cat#

(10) Nitro-Deficient Niclosamide Confers Reduced Genotoxicity and Retains Mitochondrial Uncoupling Activity for Cancer TherapyInternational Journal of Molecular SciencesSeptember 27, 2021Tsz Wai Ngai, Gamal Ahmed Elfar, Pearlyn Yeo, Nicholas Phua, Jin Hui Hor, Shuwen Chen, Ying Swan Ho, Chit Fang Cheok1:1000). PVDF membrane was subsequently washed for 10 min in 1× PBS with 0.1% Tween-20 (Promega USA H5152) 3 times prior to incubation in horseradish peroxidase-conjugated secondary antibodies (Agilent Dako

(11) Stretching DNA origami: effect of nicks and Holliday junctions on the axial stiffnessNucleic Acids ResearchDecember 2, 2020Wei-Hung Jung, Enze Chen, Remi Veneziano, Stavros Gaitanaros, Yun Chenblocking solution with 3 mg/ml bovine serum albumin (Sigma-Aldrich, A9647) and 0.1% Tween 20 (Promega, H5152) in PBS was then manually added into the flow chambers and incubated for additional 2 h. The blocking

(12) Neutral DNA–avidin nanoparticles as ultrasensitive reporters in immuno-PCRThe AnalystJuly 21, 2020Dimple Chavan, Hui Chen, Mary Crum, Binh Vu, Mohammad Safari, Maxwell Smith, Peter Vekilov, Jacinta C. Conrad, Katerina Kourentzi, Richard C. Willsontablets, pH 7.4 were from Takara Bio USA Inc. (Mountainview, CA). Tween® 20, Molecular Biology Grade (H5152) was from Promega (Madison, Wisconsin). Mx3000P optical strip tubes (401428), Mx3000P optical strip

(13) Propionic acid induces dendritic spine loss by MAPK/ERK signaling and dysregulation of autophagic fluxMolecular brainJune 2, 2020Choi H, Kim IS, Mun JY.saline (#CBTB-9110, CHEM-BIO) containing 3% BSA (#9048-4-8, GENEray Biotechnology) and 0.1% Tween 20 (H5152, Promega) for 1 h. Membranes were then washed with TBST and incubated overnight at 4 °C with primary

(14) CRISPR-on for activation of endogenous SMARCA4 and TFAP2C expression in bovine embryosReproductionJune 1, 2020Virginia Savy, Virgilia Alberio, Natalia G Canel, Laura D Ratner, Maria I Gismondi, Sergio F Ferraris, Rafael Fernandez-Martín, Jason G Knott, Romina J Bevacqua, Daniel F Salamoneimmunoreactions were blocked by incubation for 1 h in blocking solution (DPBS containing 4% BSA and 0.2% Tween® 20; H5152, Promega). Incubation with Rabbit Anti-HA tag antibody (9110 Abcam) diluted 1:300 in blocking solution,

(15) 188 MicroRNA characterization in equine induced pluripotent stem cellsReproduction, Fertility and DevelopmentJanuary 1, 2019L. N. Moro et al.After permeabilization, blocking was assessed by 1 h incubation with 3% FBS and 0.1% Tween-20 (Promega, H5152), followed by primary antibody incubation for 2 hours at room temperature. They were then washed and

(16) Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell TranscriptomicsCell stem cellDecember 6, 2018Wu H, Uchimura K, Donnelly EL, Kirita Y, Morris SA, Humphreys BD.8.0",Sigma-Aldrich,Cat#93283 1M DTT,Teknova,Cat#D9750 2M Tris pH 7.5,Sigma-Aldrich,Cat#T2944 "Tween 20, RNase Free",Promega,Cat#H5152 NxGen RNAse Inhibitor,Lucigen,Cat#30281-2 Nuclei EZ Lysis Buffer,Sigma-Aldrich,Cat#N-3408 RNasin

(17) MicroRNA characterization in equine induced pluripotent stem cellsPLOS ONEDecember 3, 2018Lucia Natalia Moro et al.After permeabilization, blocking was assessed by 1 h incubation with 3% FBS and 0.1% Tween-20 (Promega, H5152), followed by primary antibody incubation for 2 hours at room temperature. They were then washed and

(18) ICG-001 affects DRP1 activity and ER stress correlative with its anti-proliferative effectOncotargetDecember 5, 2017Zinecker H, Ouaret D, Ebner D, Gaidt MM, Taylor S, Aulicino A, Jagielowicz M, Hornung V, Simmons A.binding (Costar, cat. no. 3922), Anti-mouse DRP1 (Abcam, cat. no. ab56788), Tween 20 (Promega, cat. no. H5152), ECL™ Blocking Agent (Amersham, cat. no. RPN2125), P-DRP1Ser616 (Cell signaling technology, cat. no.

(19) Abrogating Mitochondrial Dynamics in Mouse Hearts Accelerates Mitochondrial SenescenceCell MetabolismDecember 5, 2017Moshi Song, Antonietta Franco, Julie A. Fleischer, Lihong Zhang, Gerald W. Dornphosphate-buffered saline (PBS, Thermo Fisher Scientific, 70011-069) containing 0.1% Tween-20 (Promega, H5152) plus 5% nonfat dry milk (Bio-Rad, 170-6404) and 1% bovine serum albumin (BSA, Santa Cruz Biotech, 9048-46-8)

(20) Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expressionREPRODUCTIONJuly 1, 2015L N Moro, M I Hiriart, C Buemo, J Jarazo, A Sestelo, D Veraguas, L Rodriguez-Alvarez, D F SalamoneNon-specific immunoreactions were blocked by 30 min incubation with 3% v/v FBS and 0.1% v/v Tween-20 (Promega, H5152) in DPBS (blocking solution). Incubation with the primary antibody against OCT4 (goat polyclonal IgG,

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