Fig 1: Dot blotting and FXYD5 antibody validation (HPA010817 and SC-166782). (A) Shows dot blotting of 6 × 5 fibre segments incubated with either an MHC1 or MHC2a antibody. Each dot was evaluated as no signal (?), a weak signal (?), or a clear signal (?). (B,C) HPA010817 (B) and SC-166782 (C) antibodies showed a clear multiple-band signal (19–40 kDa and 22–40 kDa, respectively) in FXYD5 overexpression samples (OE) compared to the control (C) and human muscle lysates. (D) In the first two lanes, one segment of type I (T1) and type IIa fibre (T2), respectively, was loaded. In the third lane, two pooled type IIa fibres were loaded, while pooled segments from three different fibres were loaded in lane four. In lane five, 3 µg human skeletal muscle lysate was loaded. The membrane was incubated with HPA010817. (E) The stain-free image is shown from loaded human skeletal muscle lysates either non-heated (NH) or heated for 3 min at 96 °C (96 °C) and FXYD5 OE and C samples. (F) Shows the membrane after incubation of the samples from (E) with an anti-actin antibody.
Fig 2: Abundance of Na, K-ATPase (NKA) subunits in human muscle type I and IIa fibre pools before (Pre) and after (Post) 6 weeks of high-intensity interval training (HIIT) 3 times weekly in young males (n = 8–9). (A) Frequency distribution of FXYD5 abundance in human type I and IIa muscle fibres before log-transformation. (B) Effect of HIIT on log-FXYD5 abundance. (C) Pearson’s correlation between log-FXYD5 abundance and maximal oxygen consumption (VO2max). (D,E) Effect of HIIT on log-NKAa2 (D) and log-NKAß1 abundance (E). Bars represent mean values.
Fig 3: FXYD5 abundance in human skeletal muscle type I and IIa fibres of healthy young females (n = 15) and males (n = 15). (A) Frequency distribution of FXYD5 abundance before log-transformation. (B) Individual data points for both sexes and fibre types. Bars represent sample means.
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