Fig 1: HF-MSCs relieved liver pathological damage and improved liver function.(A, B) HE staining and Masson staining in the three groups (n = 6). (C–E) Comparison of liver serological indices ALT, AST, and ALB in the three groups (n = 6). Scale bar (A, B) 50 µm. All data are shown as the means ± SD (One-way ANOVA, n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.
Fig 2: PKH67-labeled HF-MSCs homed to the damaged liver and expressed hepatocyte-specific surface markers.(A, E) PKH67-labeled HF-MSCs existed in the injured liver and are shown in green (n = 6). (B, F) Hepatocytes expressing the surface markers ALB and CK18 are shown in red (n = 6). (C, D, G, H) PKH67-stained HF-MSCs were colocalized with the hepatocyte-specific surface markers ALB and CK18 (n = 6). a–h show partially enlarged views of A–H. (I–L) PKH67-labeled HF-MSCs were rarely seen in the intestine, lung, spleen and kidney (n = 6). (M) Pearson’s correlation and the overlap coefficient of liver sections costained with ALB and CK18. (N) After transplanting the DiR-labeled HF-MSCs for 48 h, the HF-MSCs were mostly gathered in the lung and the liver. Scale bar: 50 µm (A–H), 200 µm (I–L).
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