gentleMACS M Tubes (25x) from Miltenyi Biotec

Product Specs
ItemgentleMACS M Tubes (25x)
CompanyMiltenyi Biotec
Price Pricing Info Get Pricing Get Quote for this Product
Catalog Number130-093-236
VolumeThe tubes can be used for a wide range of sample volumes from 300 µL to 10 mL.
Regulatory StatusFor research use only
ApplicationsSample Preparation
Quantity25 pc

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Local Office

Miltenyi Biotec

Miltenyi Biotec Inc.

2303 Lindbergh Street

Auburn, CA 95602

United States

Phone: Phone: +1 530 888 8871
Toll Free: 800 FOR MACS
Fax: +1 530 888 8925

Email: macs@miltenyibiotec.com

Company Profile

Website: www.miltenyibiotec.com

International Office

Miltenyi Biotec

Miltenyi Biotec B.V. & Co. KG

Friedrich-Ebert-Straße 68

51429 Bergisch Gladbach

Germany

Phone: +49 2204 8306-0
Fax: +49 2204 8306-235

Email: macs@miltenyibiotec.de

Company Profile

Website: www.miltenyibiotec.com

(1) Differential regulation of MYC expression by PKHD1/Pkhd1 in human and mouse kidneys: phenotypic implications for recessive polycystic kidney diseaseFrontiers in Cell and Developmental BiologyNovember 17, 2023Naoe Harafuji, Chaozhe Yang, Maoqing Wu, Girija Thiruvengadam, Heather Gordish-Dressman, R. Griffin Thompson, P. Darwin Bell, Avi Z. Rosenberg, Claudia Dafinger, Max C. Liebau, et al.14-day-old male mice were snap frozen, transferred to a gentleMACS M tubes (Miltenyi Biotec, Cat. No. 130-093-236, RRID:SCR_020269) in Buffer RLT plus 2-Mercaptoethanol (as per RNeasy Mini Kit instructions, Qiagen,

(2) A Mosaic Nanoparticle Vaccine Elicits Potent Mucosal Immune Response with Significant Cross‐Protection Activity against Multiple SARS‐CoV‐2 SublineagesAdvanced ScienceSeptember 1, 2023Xiantao Zhang, Shijian Wu, Jie Liu, Ran Chen, Yongli Zhang, Yingtong Lin, Zhihui Xi, Jieyi Deng, Zeyu Pu, Chaofeng Liang, et al.challenged hACE2‐K18 mice were collected and homogenized with gentleMACS M tubes (Miltenyi Biotec, 130‐093‐236) in a gentle‐MACS dissociator (Miltenyi Biotec, 130‐093‐235). RNAs were extracted using RNeasy Mini

(3) Engineering a versatile and retrievable cell macroencapsulation device for the delivery of therapeutic proteinsiScienceAugust 18, 2023Julien Grogg, Remi Vernet, Emily Charrier, Muriel Urwyler, Olivier Von Rohr, Valentin Saingier, Fabien Courtout, Aurelien Lathuiliere, Nicolas Gaudenzio, Adrien Engel, et al.preparation Punch biopsies were mechanically dissociated in gentleMACS M Tubes (Miltenyi Biotech, 130-093-236) using the GentleMACS protein program. Tissue samples were processed in 500 μL of T-PER Tissue Protein Extraction Reagent

(4) Transplantation elicits a clonally diverse CD8+ T cell response that is comprised of potent CD43+ effectorsCell ReportsAugust 16, 2023Gregory S. Cohen, Melissa A. Kallarakal, Sahana Jayaraman, Francis I. Ibukun, Katherine P. Tong, Linda D. Orzolek, H. Benjamin Benjamin Larman, Scott M. Krummey130-048-801 LS magnetic columns,Miltenyi Biotec,Cat# 130-042-401 gentleMACS M Tubes,Miltenyi Biotec,Cat# 130-093-236 CountBright Absolute Counting Beads,ThermoFisher Scientific,Cat# C36950 KEY RESOURCES TABLE

(5) Tumor-localized catalases can fail to alter tumor growth and transcriptional profiles in subcutaneous syngeneic mouse tumor modelsRedox BiologyAugust 1, 2023Allison Sheen, Yash Agarwal, Keith M. Cheah, Sarah C. Cowles, Jordan A. Stinson, Joseph R. Palmeri, Hadley D. Sikes, K. Dane Wittrupmm2. RNA purification, sequencing, and analysis. Whole tumor tissue was homogenized and lysed using gentleMACS M tubes (Miltenyi Biotec) and the RNA_01 program. After homogenization/lysis, samples were run through QiaShredder

(6) Wild mouse gut microbiota limits initial tuberculosis infection in BALB/c micePLOS ONEJuly 26, 2023Min Xie, Chen-Yu Tsai, Zachary L. McAdams, Myo Oo, Mark Hansen, Maureen Dougher, Alexander Sansano, Anderson Watson, Katherine LoMauro, Rosleine Antilus-Sainte, et al.were aseptically collected. Half of the spleen and the left lung lobe were homogenized individually in gentleMACS M tubes (Miltenyi) with 1 ml PBS/0.05% Tween 80 using a gentleMACS Octo Dissociator (Miltenyi). Subsequently,

(7) Identifying high-grade serous ovarian carcinoma-specific extracellular vesicles by polyketone-coated nanowiresScience advancesJuly 7, 2023Yokoi A, Ukai M, Yasui T, Inokuma Y, Hyeon-Deuk K, Matsuzaki J, Yoshida K, Kitagawa M, Chattrairat K, Iida M, et al.proteins, small specimens of normal uterine and ovarian tissue were shredded and crushed using 7 M urea in gentleMACS M tubes (Miltenyi Biotec) with the gentleMACS Dissociator (Miltenyi Biotec) in protein mode. The procedure was

(8) Extensive diversity in RNA termination and regulation revealed by transcriptome mapping for the Lyme pathogen Borrelia burgdorferiNature CommunicationsJuly 4, 2023Emily Petroni, Caroline Esnault, Daniel Tetreault, Ryan K. Dale, Gisela Storz, Philip P. Adamswere homogenized in ~1 ml TE buffer (10 mM Tris, 1 mM EDTA, pH 8.0) containing 0.5 mg/ml lysozyme in gentleMACS M Tubes using a gentleMACS Dissociator (program RNA_02; Miltenyi Biotech). RNA was isolated using a hot phenol/SDS

(9) Hardwiring tissue-specific AAV transduction in mice through engineered receptor expressionNature methodsJuly 1, 2023Zengel J, Wang YX, Seo JW, Ning K, Hamilton JN, Wu B, Raie M, Holbrook C, Su S, Clements DR, et al.and SELECTIV-WB mice after euthanasia. Organs were placed into gentleMACS M Tubes (Miltenyi Biotec, 130-093-236) with 3–5 ml of DMEM and lysates were made using gentleMACS Octo Dissociator (Miltenyi Biotec, 130-095-937).

(10) Caspase-8 inhibition improves the outcome of bacterial infections in mice by promoting neutrophil activationCell Reports MedicineJuly 1, 2023Germana Lentini, Agata Famà, Giuseppe Valerio De Gaetano, Francesco Coppolino, Ahlem Khachroub Mahjoub, Liv Ryan, Egil Lien, Terje Espevik, Concetta Beninati, Giuseppe TetiTC-Treated Multiple Well Plates,Sigma-Aldrich,SKUCLS3524 GentleMACS™ M Tubes,Miltenyi Biotec.,Order no. 130-093-236 GentleMACS™ Dissociator,Miltenyi Biotec.,Order no. 130-093-235 Nanodrop 2000 spectrophotometry,ThermoFisher

(11) Attenuated Mycobacterium tuberculosis vaccine protection in a low-dose murine challenge modeliScienceJune 16, 2023Samuel J. Vidal, Daniel Sellers, Jingyou Yu, Shoko Wakabayashi, Jaimie Sixsmith, Malika Aid, Julia Barrett, Sage F. Stevens, Xiaowen Liu, Wenjun Li, et al.for low dose challenge right and left lung lobes were dissected separately. Tissues were placed into gentleMACS M Tubes (Miltenyi Biotec 130-096-335) containing 5 mL of PBS and mechanically dissociated using a gentleMACS Dissociator

(12) CD40 Signaling in Mice Elicits a Broad Antiviral Response Early during Acute Infection with RNA VirusesVirusesJune 12, 2023Kai J. Rogers, Paige T. Richards, Zeb R. Zacharias, Laura L. Stunz, Rahul Vijay, Noah S. Butler, Kevin L. Legge, Gail A. Bishop, Wendy Maurydissociated for 1 min in 1ml TRIzol using the gentleMACS tissue dissociation system with associated gentleMACS M tubes (Miltenyi Biotec, Bergishch Gladbach, Germany). A total of 1 µg of RNA was subsequently converted to

(13) Protective RNA nanovaccines against Mycobacterium avium subspecies hominissuisFrontiers in ImmunologyJune 8, 2023Bubacarr J.B. Touray, Mostafa Hanafy, Yashdeep Phanse, Rachel Hildebrand, Adel M. Talaatflow cytometric assessment. Briefly, tissues were excised and placed in a gentleMACS dissociator M Tube (Miltenyi 130-093-236, Bergisch Gladbach, Germany) with 3 mL collagenase B (1 mg/mL) (Roche, Basel, Switzerland) and incubated

(14) β3-adrenergic receptor on tumor-infiltrating lymphocytes sustains IFN-γ-dependent PD-L1 expression and impairs anti-tumor immunity in neuroblastomaCancer gene therapyJune 1, 2023Bruno G, Nastasi N, Subbiani A, Boaretto A, Ciullini Mannurita S, Mattei G, Nardini P, Della Bella C, Magi A, Pini A, et al.mechanically homogenized with gentleMACS Octo Dissociator (Miltenyi Biotec, Gladbach, Germany) using gentleMACS M Tubes (Miltenyi Biotec, Gladbach, Germany). Total RNA extraction was performed with the RNeasy Plus Mini kit

(15) Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAsNAR cancerJune 1, 2023Arnesen S, Polaski JT, Blanchard Z, Osborne KS, Welm AL, O'Connell RM, Gertz J.mg of tissue per sample was homogenized in RLT plus lysis buffer with 1% beta-mercaptoethanol using gentleMACS M-tubes and a gentleMACS Octo Dissociator (Miltenyi Biotec). RNA extraction for both T-47D and PDX samples was

(16) Fibroblasts are a site of murine cytomegalovirus lytic replication and Stat1-dependent latent persistence in vivoNature CommunicationsMay 29, 2023Katarzyna M. Sitnik, Fran Krstanović, Natascha Gödecke, Ulfert Rand, Tobias Kubsch, Henrike Maaß, Yeonsu Kim, Ilija Brizić, Luka Čičin-Šainincubator under 5% CO2 and at 37 °C for 7 days without replacing the medium. Cell lysates prepared in gentleMACS M Tubes for tissue homogenisation using gentleMACS Dissociator (Miltenyi Biotech, program “mlung_02”) were assayed

(17) Evaluation of Mycobacterium avium subsp. paratuberculosis isocitrate lyase (IcL) and ABC transporter (BacA) knockout mutants as vaccine candidatesFrontiers in Cellular and Infection MicrobiologyMarch 30, 2023Razieh Eshraghisamani, Rakel Arrazuria, Lucy Luo, Jeroen De Buckgram of tissue was dissociated in 1 ml of TE buffer (10 mM TRIS-chloride, 1 mM EDTA, pH 8.0), using GentleMACS M tubes and Miltenyi dissociator, and incubated for 30 min. Supernatant were moved to a clean Eppendorf tube.

(18) EGFR inhibition leads to enhanced desmosome assembly and cardiomyocyte cohesion via ROCK activationJCI insightMarch 22, 2023Shoykhet M, Dervishi O, Menauer P, Hiermaier M, Moztarzadeh S, Osterloh C, Ludwig RJ, Williams T, Gerull B, Kääb S, et al.SDS lysis buffer was added and the samples were transferred into gentleMACS M-tubes (Miltenyi Biotec, 130-093-236) and dissociated using the protein_01_01 program of the gentleMACS OctoDissociator (Miltenyi Biotec,

(19) Demonstrating a link between diet, gut microbiota and brain: 14C radioactivity identified in the brain following gut microbial fermentation of 14C-radiolabeled tyrosine in a pig modelFrontiers in NutritionMarch 9, 2023Margaret Murray, Christopher K. Barlow, Scott Blundell, Mark Buecking, Anne Gibbon, Bernd Goeckener, Lisa M. Kaminskas, Patricia Leitner, Sophie Selby-Pham, Andrew Sinclair, et al.scintillation vials and were purchased from Perkin Elmer Pty. Ltd. (Glen Waverley, VIC, Australia). GentleMACS™ M tubes were obtained from Miltenyi Biotec Pty. Ltd. (Macquarie Park, NSW, Australia). Hydrogen peroxide was

(20) Leishmania allelic selection during experimental sand fly infection correlates with mutational signatures of oxidative DNA damageProceedings of the National Academy of SciencesMarch 7, 2023Giovanni Bussotti, Blaise Li, Pascale Pescher, Barbora Vojtkova, Isabelle Louradour, Katerina Pruzinova, Jovana Sadlova, Petr Volf, Gerald F. Späthphosphate-buffered saline (PBS) supplemented with 2.5 mg/mL saponin using the gentleMACS homogenizer with gentleMACS M tubes from Miltenyi Biotec. Amastigotes were purified as previously described (21) for DNA extraction, differentiation

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M Tubes

gentleMACS M Tubes (25x) from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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gentleMACS M Tubes (25x) from Miltenyi Biotec

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