Millicell® Standing Cell Culture Inserts - pore size 0.4 µm, diam. 12 mm, transparent PTFE membrane, hydrophilic, size 24 wells, sterile from MilliporeSigma

Product Specs
ItemMillicell^® Standing Cell Culture Inserts - pore size 0.4 µm, diam. 12 mm, transparent PTFE membrane, hydrophilic, size 24 wells, sterile
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberPICM01250
Quantity50
ApplicationsCell Culture
MaterialPolystyrene
Pore Size0.4 µm
Brand/TrademarkMillicell
TypeInsert
Sterilitysterile

Specifications/Features

Filter:
CM

Height:
10.5 mm

Sterile / Non-Sterile:
Sterile

Filter Diameter:
12 mm

Configuration:
24-well plate

Packaging:
Individually blister packaged

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) An extra-genital cell population contributes to urethra closure during mouse penis developmentbioRxivNovember 16, 2023Ciro Maurizio Amato, Xin Xu, Humphrey Hung-Chang Yaolack of urethra closure. The selected sections were placed in a 0.4μm cell culture insert (Millipore PICM01250) that was saturated with slice culture media. Slice culture media consisted of phenol-red free DMEM

(2) Episodic live imaging of cone photoreceptor maturation in GNAT2-EGFP retinal organoidsDisease Models & MechanismsNovember 1, 2023Jinlun Bai, David S. Koos, Kayla Stepanian, Zachary Fouladian, Dominic W. H. Shayler, Jennifer G. Aparicio, Scott E. Fraser, Rex A. Moats, David CobrinikGS312) on a MillicellTM 12 mm cell culture insert with 0.4 µm hydrophilic PTFE membrane (Sigma-Aldrich, PICM01250). The cell culture insert was then submerged in RO medium in a 24-well plate, and medium was changed

(3) Investigation of Conjunctival Goblet Cell and Tear MUC5AC Protein in Patients With Graves’ OphthalmopathyTranslational Vision Science & TechnologyOctober 27, 2023Yayan You, Jin Chen, Hua Chen, Jiasong Wang, Huatao Xie, Xiaohuan Pi, Xinghua Wang, Fagang Jiangwere collected from the same temporal bulbar conjunctiva using Millicell Cell Culture Inserts (0.4 µm, PICM01250; MilliporeSigma, Burlington, MA) under topical anesthesia (0.4% Benoxil). The insert was gently pressed

(4) Guanine quadruplexes mediate mitochondrial RNA polymerase pausingbioRxivOctober 22, 2023Ryan Snyder, Don Delker, Joshua T Burdick, Vivian G. Cheung, Jason A. Wattsfree media were seeded on the bottom of collagen-coated permeable transwell membranes (EMD Millipore, #PICM01250) and allowed to form epithelial monolayers. The integrity of the cellular barrier was monitored daily

(5) Bacterial contact induces polar plug disintegration to mediate whipworm egg hatchingPLOS PathogensSeptember 22, 2023Amicha Robertson, Joseph Sall, Mericien Venzon, Janet J. Olivas, Xuhui Zheng, Michael Cammer, Noelle Antao, Chunyi Zhou, Joseph C. Devlin, Rafaela Saes Thur, et al.cacodylate buffer (CB, pH 7.2) at 4°C for a week. ~50 eggs were then loaded into 12 mm, 0.44 mm transwells (#PICM01250, Millipore Sigma) to avoid losing eggs during sample processing. The eggs were washed 3 times with PBS,

(6) Blockage of STAT3 during epileptogenesis prevents GABAergic loss and imprinting of the epileptic stateBrainAugust 1, 2023Soraya Martín-Suárez, Jesús María Cortes, Paolo Bonifaziwith 5% CO2.18 After slicing, the slices were transferred to 0.4 μm culture plate inserts (Millipore, PICM01250). The membranes were placed in 24-well plates, each well containing 250 µl of culture medium. The medium

(7) In-Depth Analysis of the Pancreatic Extracellular Matrix during Development for Next-Generation Tissue EngineeringInternational Journal of Molecular SciencesJune 17, 2023Laura Glorieux, Laura Vandooren, Sylvie Derclaye, Sébastien Pyr dit Ruys, Paloma Oncina-Gil, Anna Salowka, Gaëtan Herinckx, Elias Aajja, Pascale Lemoine, Catherine Spourquet, et al.seeding. Afterwards, tissues were placed on microporous membranes (Millipore, Burlington, MA, USA, PICM01250) with 330 µL of medium underneath. One-million E14tg2A or PANC-1 cells were seeded on top of the decellularized

(8) Singularity response reveals entrainment properties in mammalian circadian clockNature CommunicationsMay 17, 2023Kosaku Masuda, Naohiro Kon, Kosuke Iizuka, Yoshitaka Fukada, Takeshi Sakurai, Arisa Hiranoout from mice at ZT6 to ZT7, and each tissue piece was placed on a cell culture insert (catalog no. PICM01250, Merck KGaA, Darmstadt, Germany) in a 24-well plate with 500 μl of the recording medium (phenol-red-free

(9) Androgen receptor signaling regulates follicular growth and steroidogenesis in interaction with gonadotropins in the ovary during mini-puberty in miceFront Endocrinol (Lausanne)April 19, 2023Marie M. Devillers, Charlotte M. François, Mélanie Chester, Raphaël Corre, Victoria Cluzet, Frank Giton, Joëlle Cohen-Tannoudji, Céline J. Guigonpostnatal ovaries Ovaries from 13 dpn female mice were placed on cell culture inserts (Millicell #PICM01250, Millipore, Guyancourt, France) on the top of 400 µl of RPMI culture medium without red phenol containing

(10) Bacterial contact induces polar plug disintegration to mediate whipworm egg hatchingbioRxivMarch 18, 2023Amicha Robertson, Joseph Sall, Mericien Venzon, Janet J. Olivas, Xuhui Zheng, Michael Cammer, Noelle Antao, Rafaela Saes Thur, Jeffrey Bethony, Peter Nejsum, et al.cacodylate buffer (CB, pH 7.2) at 4°C for a week. ~50 eggs were then loaded into 12 mm, 0.44 mm transwells (#PICM01250, Millipore Sigma) to avoid losing eggs during sample processing. The eggs were washed 3 times with PBS,

(11) Episodic live imaging of cone photoreceptor maturation in GNAT2-EGFP retinal organoidsbioRxiv : the preprint server for biologyMarch 1, 2023Bai J, Koos DS, Stepanian K, Fouladian Z, Shayler DWH, Aparicio JG, Fraser SE, Moats RA, Cobrinik D.(Advanced Biomatrix, #GS312) on Millicell™ 12 mm cell culture insert with 0.4 μm hydrophilic PTFE membrane (Sigma, #PICM01250). The cell culture insert was then submerged in RO media in a 24-well plate, and media was changed following

(12) Protocol to culture and image pancreatic ductal adenocarcinoma tumor slices to study T cell migrationSTAR ProtocolsMarch 1, 2023Nelson J. Rodríguez-Merced, Mackenzie K. Callaway, Paolo P. ProvenzanoTechnologies/Bruker,N/A "Millicell Cell Culture Insert, 12 mm, hydrophilic PTFE, 0.4 μm",MilliporeSigma,Cat# PICM01250 "Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 μm",MilliporeSigma,Cat# PICM0RG50 Slice

(13) Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cellsCell Structure and FunctionFebruary 8, 2023Shinji C. Nagasaki, Tomonori D. Fukuda, Mayumi Yamada, Yusuke III Suzuki, Ryo Kakutani, Adam T. Guy, Itaru Imayoshivibratome (Leica, Wetzlar, DE, VT1000), transferred to 12-mm well culture insert (Merck, Darmstadt, DE, PICM01250), and cultured in slice culture medium, DMEM/F-12 (GIBCO, Waltham, USA, 11039) supplemented with 0.6

(14) TAp63 determines the fate of oocytes against DNA damageScience AdvancesDecember 21, 2022Yi Luan, Seok-Yeong Yu, Amirhossein Abazarikia, Rosemary Dong, So-Youn Kimisolated from the ovarian bursa. Then, the ovaries were cultured on 0.4-μm Millicell cell culture inserts (PICM01250, MilliporeSigma) with a culture medium composed of minimum essential medium α (32571036, Gibco) supplemented

(15) Evaluation of neuroprotective and immunomodulatory properties of mesenchymal stem cells in an ex vivo retinal explant modelJournal of NeuroinflammationDecember 1, 2022Élodie Reboussin, Juliette Buffault, Françoise Brignole-Baudouin, Annabelle Réaux-Le Goazigo, Luisa Riancho, Céline Olmiere, José-Alain Sahel, Stéphane Mélik Parsadaniantz, Christophe Baudouinup on Millicell-Polytetrafluoroethylene (PTFE) 0.4-μm culture plate inserts (Merck Millipore, ref. PICM01250), in culture medium composed of Neurobasal A (Thermo Fisher Scientific, ref. 10888022), 2% B27 supplement

(16) A TGFβR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cellsScientific reportsSeptember 2, 2022Fowler EW, van Venrooy EJ, Witt RL, Jia X.expression was conducted using 100 μL hydrogels prepared in 12 mm Millicell 04 μm PTFE cell culture inserts (PICM01250; EMD Millipore) with 800 μL HeptoSTIM media. ICC characterization was conducted using 30 μL hydrogels

(17) Oocyte CTR1 is not essential for cisplatin-induced oocyte death of primordial follicleMicroPubl BiolSeptember 1, 2022Seok-Yeong Yu, Yi Luan, Amirhossein Abazarikia, Rosemary Dong, Jaekwon Lee, So-Youn Kimisolated ovaries from mice at PD8 were cultured on 0.4 μm Millicell Cell Culture Inserts (MilliporeSigma, PICM01250) in a 24-well plate in the Minimum Essential Medium α (Gibco, 32571036) supplemented with 1 mg/ml bovine

(18) Androgen receptor variant-7 regulation by tenascin-c induced src activationCell Communication and SignalingAugust 10, 2022Rintu Thomas, John Michael Jerome, Truong D. Dang, Eric P. Souto, Joshua N. Mallam, David R. Rowleydensity of 2.0E5 total cells/cm2 to form heterotypical organoids on millicell-CM cell culture inserts (#PICM01250) from Sigma. TNC antibody was conjugated to FITC (abcam # ab188285) based on recommended protocol. Organoid culture

(19) A cAMP signalosome in primary cilia drives gene expression and kidney cyst formationEMBO reportsAugust 3, 2022Hansen JN, Kaiser F, Leyendecker P, Stüven B, Krause JH, Derakhshandeh F, Irfan J, Sroka TJ, Preval KM, Desai PB, et al.was equipped with 400 µl/well of medium and a Millicell® Cell Culture Insert (Merck Millipore Ltd., #PICM01250). Per condition, 1.6 × 105 cells in 40 µl of medium were mixed 1:2 with a 1:1 mixture of collagen I

(20) Identification and implication of tissue-enriched ligands in epithelial-endothelial crosstalk during pancreas developmentScientific reportsJuly 21, 2022Moulis M, Runser SVM, Glorieux L, Dauguet N, Vanderaa C, Gatto L, Tyteca D, Henriet P, Spagnoli FM, Iber D, et al.three washes in culture medium, E12.5 pancreatic dorsal buds were placed on microporous membranes (PICM01250, Millipore) at the air-medium interface. DMEM/F-12 medium (11039-021, Gibco) with 10% serum, 100 U/mL

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Millicell^® Standing Cell Culture Inserts - pore size 0.4 µm, diam. 12 mm, transparent PTFE membrane, hydrophilic, size 24 wells, sterile from MilliporeSigma

Description

Specifications/Features

MilliporeSigma

Citations (113)

Reviews

Millicell^® Standing Cell Culture Inserts - pore size 0.4 µm, diam. 12 mm, transparent PTFE membrane, hydrophilic, size 24 wells, sterile from MilliporeSigma

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