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TaqI
TaqI-v2
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TaqI-v2
TaqI-v2 from New England Biolabs
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Citations:
(15)
Supplier Page
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New England Biolabs for
TaqI-v2
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Company Info
Citations
Reviews
Product Specs
Item
TaqI-v2
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0149S
Quantity
4000 units
Concentration
20-100 u/µL
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
T/CGA
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (15)
(1)
Exploring the Influence of VDR Genetic Variants TaqI, ApaI, and FokI on COVID-19 Severity and Long-COVID-19 Symptoms
Journal of Personalized Medicine
November 28, 2023
Ghayda’ Alhammadin, Yazun Jarrar, Abdalla Madani, Su-Jun Lee
Korea). For the
PCR-RFLP
analysis, the restriction enzymes
FokI
(cat. no.
R0109S
) and
TaqI-v2
(cat. no.
R0149S
) were obtained from
New England Biolabs
(Ipswich, MA, USA);
ApaI
(cat. no.
R6361
) was purchased from
(2)
The maize gene
maternal derepression of r1
encodes a DNA glycosylase that demethylates DNA and reduces siRNA expression in the endosperm
The Plant Cell
September 27, 2022
Jonathan I Gent, Kaitlin M Higgins, Kyle W Swentowsky, Fang-Fang Fu, Yibing Zeng, Dong won Kim, R Kelly Dawe, Nathan M Springer, Sarah N Anderson
mdr1 was amplified by
PCR
with primers W22-Ros1-F1 and W22-Ros1-R1 and digested with
TaqI-v2
(
NEB
#
R0149S
). See Supplemental Table S2 for primer sequences. The different mdr1 alleles could be distinguished
(3)
The Mechanosensitive Ion Channel MSL10 Potentiates Responses to Cell Swelling in Arabidopsis Seedlings
SSRN Electronic Journal
April 3, 2022
Debarati Basu, Elizabeth S. Haswell
microplates, clear Greiner Bio-One Cat# 651161
TaqI-v2
, restriction enzyme
New England Biolabs
Cat#
R0149L
Immobilon-P PVDF transfer
membrane
EMD Millipore
Cat#
IPVH00010
Other RESOURCE AVAILABILITY Lead
(4)
Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle
Journal of Cell Biology
December 6, 2021
Harriet P. Lo et al.
confirmed by
Sanger sequencing
(Australian Genome Research Facility, Brisbane) or digestion with αTaqI (#
R0149S
;
NEB
). Morphometrics of live
zebrafish
Images of anesthetized embryos were captured on an Olympus
(5)
ROR2 Is Epigenetically Regulated in Endometrial Cancer
Cancers (Basel)
January 21, 2021
Dongli Liu, Luis Enriquez, Caroline E. Ford
(F-AGGAAATGTTTAGGA AAATAAATAGGT, R-AAAACAAACAACTAAAATACTAAAAA) and digested with TaqI restriction enzyme mix (#
R0149S
,
NEB
, Ipswich, MA, USA). The targeted region as well as the two CpG sites (cg03900646 and cg14145355)
(6)
The Mechanosensitive Ion Channel MSL10 Potentiates Responses to Cell Swelling in Arabidopsis Seedlings
Current Biology
July 1, 2020
Debarati Basu, Elizabeth S. Haswell
microplates, clear Greiner Bio-One Cat# 651161
TaqI-v2
, restriction enzyme
New England Biolabs
Cat#
R0149L
Immobilon-P PVDF transfer
membrane
EMD Millipore
Cat#
IPVH00010
Other RESOURCE AVAILABILITY Lead
(7)
Anosmin1 Shuttles Fgf to Facilitate Its Diffusion, Increase Its Local Concentration, and Induce Sensory Organs
Developmental Cell
September 1, 2018
John Wang, Yandong Yin, Stephanie Lau, Jagadish Sankaran, Eli Rothenberg, Thorsten Wohland, Martin Meier-Schellersheim, Holger Knaut
,
New England Biolabs
,
R0620L
AciI
,
New England Biolabs
,
R0551S
ApoI
,
New England Biolabs
,
R0566L
Taqαi,
New England Biolabs
,
R0149L
Brefeldin A,
Sigma Aldrich
,
B7651
–5MG Critical Commercial
Assays
,Critical Commercial
Assays
,Critical
(8)
Imprinted gene dysregulation in a
Tet1
null mouse model is stochastic and variable in the germline and offspring
Development
January 1, 2018
Jennifer M. SanMiguel, Lara K. Abramowitz, Marisa S. Bartolomei
inactivation; Peg3,
MnlI
(
NEB
,
R0163S
), 37°C for 3 h, heat inactivated at 65°C for 20 min; Cdkn1c, Taq αI (
NEB
,
R0149S
), 65°C for 1 h, heat inactivated for 20 min at 80°C; Kcnq1ot1,
StuI
(
NEB
,
R0187S
), 37°C for 3 h, no
(9)
Base-resolution profiling of active DNA demethylation using methylase-assisted bisulfite sequencing (MAB-seq) and caMAB-seq
Nature Protocols
June 1, 2016
Hao Wu, Xiaoji Wu, Yi Zhang
EN0531
)
Anti-H3K4me1
(10 μg/mL;
Abcam
, cat. no.
ab8895
) TaqαI (20 U/μL;
New England Biolabs
, cat. no.
R0149L
)
Klenow fragment (exo–, 5 U/μL
;
Thermo Scientific
, cat. no.
EP0422
)
T4 DNA ligase
(2000 U/μL;
New England Biolabs
(10)
Mitochondrial Involvement in Vertebrate Speciation? The Case of Mito-nuclear Genetic Divergence in Chameleons
Genome Biology and Evolution
December 1, 2015
Dan Bar-Yaacov, Zena Hadjivasiliou, Liron Levin, Gilad Barshad, Raz Zarivach, Amos Bouskila, Dan Mishmar
restriction mix and conditions): POLRMT (mutation at position 1218)—both TaqαI (
New England BioLabs
#
R0149S
) and
MlucI
(
New England Biolabs
#
R0538S
); MRPL30—
PstI
(
New England BioLabs
#
R0140S
); ETFA—
HindIII
(
Thermo
(11)
COBRA-Seq: Sensitive and Quantitative Methylome Profiling
Genes (Basel)
October 23, 2015
Hilal Varinli, Aaron L. Statham, Susan J. Clark, Peter L. Molloy, Jason P. Ross
amplified and purified 2 μg library material was then digested overnight with 100 U of TaqI (NEB, cat#
R0149S
) in
NEB
buffer 4 in a final volume of 100 μL at 65 °C. After TaqI digest, there are three main fragment
(12)
In silico analysis identifies novel restriction enzyme combinations that expand reduced representation bisulfite sequencing CpG coverage
BMC Research Notes
December 1, 2014
Daniel B Martinez-Arguelles, Sunghoon Lee, Vassilios Papadopoulos
number AluI,AG|CT,37,CutSmart,80,No,R0137S BfaI,C|TAG,37,CutSmart,80,No,R0568S HaeIII,GG|CC,37,CutSmart,80,No,R0108S
HpyCH4V
,TG|CA,37,CutSmart,65,No,
R0620S
MluCI
,|AATT,37,CutSmart,No,No,
R0538S
MseI
,T|TAA,37,CutSmart,65,No,
R0525S
MspI
,C|CGG,37,CutSmart,No,No,
R0106S
TaqI,T|CGA,65,CutSmart,80,dam,
R0149S
CviQI
,G|TAC,25,
NEB
3.1,No,No,
R0639S
CviAII
,C|ATG,25,CutSmart,65,No,
R0640S
ApeKI
,G|CWGC,75,
NEB
3.1,No,Yes,
R0643S
Properties
(13)
Association between Polymorphisms of Interleukin-17A and Interleukin-17F Genes and Silicosis Susceptibility in Chinese Han People
Asian Pacific Journal of Cancer Prevention
November 7, 2014
Ying Chen, Xue-Yun Fan, Yu-Lan Jin, San-Qiao Yao, Xiang Yun, Zheng-Bing Hua, Fu-Hai Shen
I46.8 (
New England Biolabs
, Beverly, MA, 51.1 types of fragments: 359bp, 206bp+152bp into three 51.7 USA,
R0149S
) for the A-832G, 54.2 and digested in 37℃ for and 359bp+206bp+152bp, as shown in Figure 1A. 31.3 15
(14)
Carnitine Palmitoyltransferase 1B 531K Allele Carriers Sustain a Higher Respiratory Quotient after Aerobic Exercise, but ß3-Adrenoceptor 64R Allele Does Not Affect Lipolysis: A Human Model
PLoS ONE
June 6, 2014
Eduardo Gómez-Gómez, Martín Efrén Ríos-Martínez, Elena Margarita Castro-Rodríguez, Mario Del-Toro-Equíhua, Mario Ramírez-Flores, Ivan Delgado-Enciso, Ana Lilia Pérez-Huitimea, Luz Margarita Baltazar-Rodríguez, Gilberto Velasco-Pineda, Jesús Muñiz-Murguía
(15)
Oxidative bisulfite sequencing of 5-methylcytosine and 5-hydroxymethylcytosine
Nature Protocols
October 1, 2013
Michael J Booth, Tobias W B Ost, Dario Beraldi, Neil M Bell, Miguel R Branco, Wolf Reik, Shankar Balasubramanian
GeneJet PCR purification kit
(
Thermo Scientific
, cat. no.
K0701
) Taqa1 restriction endonuclease (Taq1 RE;
NEB
, cat. no.
R0149S
) Ethanol (Sigma-Aldrich, cat. no. 32221) GelRed, 3× dye (Cambridge Bioscience, cat. no. 41001)
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TaqI-v2 from New England Biolabs
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Company Info
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