TaqI-v2 from New England Biolabs

Product Specs
ItemTaqI-v2
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0149T
Concentration100,000 units/ml
Quantity4000 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

5'-T/CGA-3'

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Suppression of inflammatory arthritis by the parasitic worm product ES-62 is associated with epigenetic changes in synovial fibroblastsPLOS PathogensNovember 8, 2021Marlene Corbet, Miguel A. Pineda, Kun Yang, Anuradha Tarafdar, Sarah McGrath, Rinako Nakagawa, Felicity E. Lumb, Colin J. Suckling, William Harnett, Margaret M. Harnett

(2) Increased burden of mitochondrial DNA deletions and point mutations in early-onset age-related hearing loss in mitochondrial mutator miceExperimental GerontologyOctober 1, 2019Mi-Jung Kim et al.(Thermo Fisher Scientific, B28) and digested by adding 100 U of TaqI restriction enzyme (New England Biolabs, R0149M) every hour over a 5-h time span. The mtDNA was then cleaned up with the Oligo Clean & Concentrator

(3) Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated virusesNature CommunicationsDecember 1, 2018Yeonsoo Yoon, Dan Wang, Phillip W. L. Tai, Joy Riley, Guangping Gao, Jaime A. Rivera-Pérezcontrol sample. Genomic material (20 μg total input) was fragmented by TaqαI digestion (NEB, Cat. No. R0149M). Fragmented DNAs were subjected to phenol–chloroform extraction and ethanol precipitation to purify

(4) Multiple Molecular Mechanisms Rescue mtDNA Disease in C. elegansCell ReportsMarch 20, 2018Suraiya Haroon et al.performed whole-genome preps. 10–20 µg of total DNA was digested with TaqαI restriction enzyme (cat. no. R0149T, New England Biolabs) for 10 hr, with 1 µL of fresh enzyme added every hour. Digested DNA was amplified

(5) Knockdown of Laminin gamma-3 (Lamc3) impairs motoneuron guidance in the zebrafish embryoWellcome Open ResearchNovember 16, 2017Alexander M. J. Eve, James C. Smith

(6) Effects of calorie restriction on the lifespan and healthspan of POLG mitochondrial mutator micePLOS ONEFebruary 3, 2017Shinichi Someya, Gregory C. Kujoth, Mi-Jung Kim, Timothy A. Hacker, Marc Vermulst, Richard Weindruch, Tomas A. Prolla2017 2 / 11 Effects of calorie restriction on the lifespan of mitochondrial mutator mice (NEB, R0149M) every hour over a 5-hour time span. The mtDNA was then cleaned up with an oligo-concentrator kit from

(7) Conservation and Divergence of Regulatory Strategies at Hox Loci and the Origin of Tetrapod DigitsPLoS BiologyJanuary 21, 2014Joost M. Woltering, Daan Noordermeer, Marion Leleu, Denis Dubouleenzyme was DpnII (New England Biolabs, R0543M), and the secondary enzyme was TaqαI (New England Biolabs, R0149M). In the latter case, DNA was cut for 8 h at 65°C. For the Tetraodon baits, assessed in transgenic mice,

(8) 25-hydroxyvitamin D deficiency, exacerbation frequency and human rhinovirus exacerbations in chronic obstructive pulmonary diseaseBMC Pulmonary MedicineDecember 1, 2012Jennifer K Quint, Gavin C Donaldson, Nancy Wassef, John R Hurst, Michael Thomas, Jadwiga A Wedzichapolymorphism (rs731236), a RFLP analysis was carried out by using the TaqIα restriction endonuclease enzyme (R0149T New England BioLabs). The reaction consisted of; 2μl buffer, 0.2μl BSA, 7.7μl dH2O, 0.1μl RE (TaqIα)

(9) Measuring the methylome in clinical samples: Improved processing of the Infinium Human Methylation450 BeadChip ArrayEpigeneticsOctober 13, 2012Hong Pan et al.restriction enzyme digestion for 2 h each by 150 U each of MspI and TaqαI (Catalog Nos.: R0106S and R0149M, New England Biolabs) at optimal temperatures, according to manufacturer’s instructions. The digested

(10) A random mutation capture assay to detect genomic point mutations in mouse tissueNucleic Acids ResearchJune 1, 2011Jocelyn H. Wright, Kristina L. Modjeski, Jason H. Bielas, Bradley D. Preston, Nelson Fausto, Lawrence A. Loeb, Jean S. Campbellaccurate. TaqI digestion of genomic DNA High concentration TaqαI (100 000 U/ml; New England Biolabs catalog# R0149M) is used to maximize enzyme concentration in the reaction. TaqαI is a mutant form of the enzyme that

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TaqI-v2

TaqI-v2 from New England Biolabs

Description

Sequence

New England Biolabs

Citations (10)

Reviews

TaqI-v2 from New England Biolabs

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