T4 DNA Ligase (HC) from Promega

Product Specs
ItemT4 DNA Ligase (HC)
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM1794
Quantity500u
Concentration10–20u/μl

Specifications/Features

Unit Definition:
0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/Hind III fragments at 16°C in 20 minutes.

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Systematic Part Transfer by Extending a Modular Toolkit to Diverse BacteriaACS Synthetic BiologyJuly 21, 2023Kevin W. Keating, Eric M. YoungBsaI (New England BioLabs R3733S) or BbsI (Thermo Scientific ER1012), 8% v/v T4 DNA ligase HC (Promega M1794), 20% v/v T4 DNA ligase buffer (Promega), 52% v/v nuclease-free water. This Mastermix was transferred

(2) Design of Four Small-Molecule-Inducible Systems in the Yeast Chromosome, Applied to Optimize Terpene BiosynthesisACS Synthetic BiologyApril 21, 2023Jong Hyun Park, Marcelo C. Bassalo, Geng-Min Lin, Ye Chen, Hamid Doosthosseini, Joep Schmitz, Johannes A. Roubos, Christopher A. Voigtthen assembled using Type IIS assembly, BsaI (NEB #R0535L), and high concentration T4 ligase (Promega #M1794) into the pDSMB backbones. Each pDSMB backbone contains upstream and downstream 50 bp connectors and

(3) Repression of HIV-1 reactivation mediated by CRISPR/dCas9-KRAB in lymphoid and myeloid cell modelsRetrovirologyDecember 1, 2022Lendel Correia da Costa, Larissa Maciel Bomfim, Uilla Victoria Torres Dittz, Camila de Almeida Velozo, Rodrigo Delvecchio da Cunha, Amilcar Tanuriusing 10 UI of NheI (Promega, WI, USA) and the resulting plasmid was relinked with 1 UI of T4 ligase (M1794, PROMEGA, WI, USA), generating the vector pdSpCas9koKRAB. The excision was confirmed by sequencing the

(4) Genetic manipulation of gut microbes enables single-gene interrogation in a complex microbiomeCellFebruary 1, 2022Wen-Bing Jin, Ting-Ting Li, Da Huo, Sophia Qu, Xin V. Li, Mohammad Arifuzzaman, Svetlana F. Lima, Hui-Qing Shi, Aolin Wang, Gregory G. Putzel, et al.enzyme,NEB,Cat# R3642S SYBR green chemistry,Applied Biosystems,Cat# 4368706 T4 DNA Ligase (HC),Promega,Cat# M1794 XbaI restriction enzyme,NEB,Cat# R0145S XhoI restriction enzyme,NEB,Cat# R0146S Experimental models:

(5) Engineered yeast genomes accurately assembled from pure and mixed samplesNature CommunicationsDecember 1, 2021Joseph H. Collins et al.Scientific, 1402-4700). Next, 1 μL of 10X Ligase Buffer and 0.4 μL of 20 U/μL T4 DNA ligase (Promega, M1794) was added to the tube. Finally, 1 1 μL of either the BbsI (L0 assembly) or BsaI (L1 assembly) enzymes

(6) Selection for constrained peptides that bind to a single target proteinNature CommunicationsDecember 1, 2021Andrew M. King et al.molar ratio (200 fmol each) along with 10 μl 10× DNA ligase buffer, 2 μl T4 DNA ligase (HC) (20 U/μl) (M1794, Promega, WI) and 4 μl SapI in 100 μl total volume. Reactions were cycled 25 times for 2 min at 37 °C

(7) P recision design of stable genetic circuits carried in highly‐insulated E. coli genomic landing padsMolecular Systems BiologyAugust 19, 2020Yongjin Park, Amin Espah Borujeni, Thomas E Gorochowski, Jonghyeon Shin, Christopher A Voigtusing Type II assembly with BsaI (New England Biolabs, USA, R3733) and T4 ligase HC (Promega, USA, M1794). The reaction mix was prepared by adding 40 fmol of each DNA fragment, 1 μl of BsaI, 0.5 μl of T4 ligase

(8) A complex regulatory landscape involved in the development of mammalian external genitalseLifeApril 17, 2020Ana Rita Amândio, Lucille Lopez-Delisle, Christopher Chase Bolt, Bénédicte Mascrez, Denis Duboulesecond restriction. All ligation steps were performed using highly concentrated T4 DNA ligase (Promega, M1794). For each viewpoint approximately 1 μg of DNA was amplified by using 12 individual PCR reactions. Libraries

(9) Biosynthesis of the nitrogenase active-site cofactor precursor NifB-co in Saccharomyces cerevisiaeProceedings of the National Academy of SciencesDecember 10, 2019Stefan Burén et al.ampicillin-resistant level-0 vector with BpiI (no. ER1012; ThermoFisher) and high-concentration T4 ligase (no. M1794; Promega). Each assembled part vector contained the part flanked by BsaI sites and 4-base pair (bp)

(10) TET enzymes control antibody production and shape the mutational landscape in germinal centre B cellsThe FEBS journalSeptember 1, 2019Schoeler K, Aufschnaiter A, Messner S, Derudder E, Herzog S, Villunger A, Rajewsky K, Labi V.Ipswich, MA, USA, R0195). The purified PCR products were ligated into the vector using T4 ligase (Promega, M1794) as per manufacturer's instructions. Using a standard transformation protocol, DH5α bacteria were transformed

(11) Microfluidics-Based Chromosome Conformation Capture (3C) Technology for Examining Chromatin Organization with a Low Quantity of CellsAnalytical ChemistryMarch 20, 2018Chen Sun, Chang LumM Tris-HCl, 10 mM MgCl2, 10 mM DTT, 1 mM ATP, pH=7.8) and 4 U T4 ligase enzyme (Promega, Cat. No. M1794) with all valves closed. Ligation reagents were diffused into the reaction chamber after opening the

(12) Flexi Vector CloningMethods in Molecular BiologyFebruary 9, 2018Paul G. Blommel, Peter A. Martin, Kory D. Seder, Russell L. Wrobel, Brian G. FoxReagents The SgfI/PmeI 10X Enzyme Blend and Buffer (Product No. R1852), high concentration T4 DNA ligase (M1794), Magnesil PCR cleanup kits (A923A), Magnebot II magnetic bead separation block (V8351), and DNA molecular

(13) Large scale genomic reorganization of topological domains at the HoxD locusGenome BiologyDecember 1, 2017Pierre J. Fabre, Marion Leleu, Benjamin H. Mormann, Lucille Lopez-Delisle, Daan Noordermeer, Leonardo Beccari, Denis Dubouleamplification according to [49]. The ligation steps were performed using high concentrated T4 DNA ligase (M1794, Promega) and the inverse PCRs for amplification were carried out using primers specific for the various

(14) Registry in a tube: multiplexed pools of retrievable parts for genetic design space explorationNucleic Acids ResearchDecember 21, 2016Lauren B. A. Woodruff, Thomas E. Gorochowski, Nicholas Roehner, Tarjei S. Mikkelsen, Douglas Densmore, D. Benjamin Gordon, Robert Nicol, Christopher A. Voigt40 fmol of mixed parts for each part position, 2.5 U BsaI, 2.5 U T4 DNA ligase (20 U/μl HC; Promega, M1794) in 1X ligase buffer with the protocol: 37°C for 5 h, 10 cycles (37°C for 2 min then 16°C for 5 min),

(15) Single molecule measurements of DNA helicase activity with magnetic tweezers and t-test based step-finding analysisMethodsAugust 1, 2016Yeonee Seol, Marie-Paule Strub, Keir C. Neumanthe hairpin main body were mixed with a 10-fold excess of T-loop DNA and ligated with T4 DNA ligase (M1794, Promega) to form the DNA hairpin substrate (Fig. 2c). 2.3.2 Gapped DNA To prepare the gapped DNA

(16) A role for HOX13 proteins in the regulatory switch between TADs at the HoxD locusGenes & DevelopmentMay 15, 2016Leonardo Beccari, Nayuta Yakushiji-Kaminatsui, Joost M. Woltering, Anamaria Necsulea, Nicolas Lonfat, Eddie Rodríguez-Carballo, Benedicte Mascrez, Shiori Yamamoto, Atsushi Kuroiwa, Denis Dubouleenzymes, respectively, and ligation steps were performed using highly concentrated T4 DNA ligase (Promega, M1794). Inverse PCRs for amplification were carried out using primers for the Hoxd11 and Hoxd13 viewpoints

(17) Regulation of miR-200c/141 expression by intergenic DNA-looping and transcriptional read-throughNature CommunicationsApril 1, 2016Luciana Batista, Brigitte Bourachot, Bogdan Mateescu, Fabien Reyal, Fatima Mechta-Grigoriouwas performed at 16 °C for 4 h followed by 30 min at room temperature using T4 ligase from Promega (# M1794). After overnight reverse crosslink at 65 °C with proteinase K (Roche, #03 115 836 001), DNA was extracted

(18) Algorithmic co-optimization of genetic constructs and growth conditions: application to 6-ACA, a potential nylon-6 precursorNucleic Acids ResearchDecember 2, 2015Hui Zhou, Brenda Vonk, Johannes A. Roubos, Roel A.L. Bovenberg, Christopher A. Voigtlevel 0 plasmid are mixed with 5 U BsaI (New England Biolabs, #R0539S) and 5 U T4 DNA Ligase (Promega, #M1794) for a total of 10 μl 1× Promega T4 DNA Ligase Buffer and incubated. Two level 1 plasmids carrying expression

(19) Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombeOpen BiologyJune 26, 2015Yasutaka Kakui, Tomonari Sunaga, Kunio Arai, James Dodgson, Liang Ji, Attila Csikász-Nagy, Rafael Carazo-Salas, Masamitsu Satoreaction [9] is as follows. For efficient reaction, we use T4 DNA ligase (HC) from Promega (catalogue #M1794) and BsaI (not HF) from New England Biolab (#R0535S). Similar enzymes by other suppliers may also work.

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T4 DNA Ligase (HC)

T4 DNA Ligase (HC) from Promega

Description

Specifications/Features

Promega

Citations (19)

Reviews

T4 DNA Ligase (HC) from Promega

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