pGEM-T Easy Vector System I from Promega

Product Specs
ItempGEM-T Easy Vector System I
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA1360
Selectable MarkerAmp
Quantity20 reactions
Nucleic Acid TypeCloning Vectors
Selectable Marker (standardized)Ampicillin

Specifications/Features

Kit Contents:
1 x 1.2µg pGEM®-T Easy Vector; 1 x 12µl Control Insert DNA; 1 x 100u T4 DNA Ligase; 1 x 200µl 2X Rapid Ligation Buffer

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Cell autonomous role of leucine-rich repeat kinase in protection of dopaminergic neuron survivalbioRxivDecember 5, 2023Jongkyun Kang, Guodong Huang, Long Ma, Youren Tong, Anu Shahapal, Phoenix Chen, Jie ShenResources Center) using primers, P3 and P4. The PCR fragment was subcloned into the pGEM-T vector (A1360, Promega) to generate pLM1 (for details, see Figure 1-figure supplement 1). The right middle homologous

(2) NSUN2-mediated M(5)c methylation of IRF3 mRNA negatively regulates type I interferon responses during various viral infectionsEmerging microbes & infectionsDecember 1, 2023Wang H, Feng J, Zeng C, Liu J, Fu Z, Wang D, Wang Y, Zhang L, Li J, Jiang A, et al.Adaptor-mut primer pairs. The PCR products were inserted into the pGEM-T Easy Vector System (Promega, A1360) following the manufacturer’s instructions. The plasmids purified from single clones were sequenced

(3) Determining the structure of protein-bound ceramides, essential lipids for skin barrier functioniScienceNovember 17, 2023Yusuke Ohno, Tetsuya Nakamura, Takafumi Iwasaki, Akira Katsuyama, Satoshi Ichikawa, Akio Kiharaprimer:5′-CTACATCTTTGAGACCACCGGTGGG-3′,Merck,N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pGEM-T Easy Vector Systems,Promega,Cat#A1360 pCE-puro-3×FLAG-1,"Kihara et al.,56 2006",N/A pCE-puro-3×FLAG-EPHX2,This study,N/A ,, Software and

(4) Affinity hierarchies and amphiphilic proteins underlie the co-assembly of nucleolar and heterochromatin condensatesResearch SquareNovember 9, 2023Srivarsha Rajshekar, Srivarsha Adame-Arana, Gaurav Adame-Arana, Gaurav Bajpai, Serafin Colmenares, Serafin Colmenares, Samuel SafranMulticolor Kit,",Thermo Fisher,F32951 Alexa Fluor™ dye combination,, pGEM T-Easy Cloning Kit,Promega,A1360 Recombinant DNA,, pGEM-rDNA,This study,N/A FISH Probes,, 359bp,This study,N/A 1.686,This study,N/A AAGAG,This

(5) Transcriptional control of motor pool formation and motor circuit connectivity by the LIM-HD protein Isl2eLifeOctober 23, 2023Yunjeong Lee, In Seo Yeo, Namhee Kim, Dong-Keun Lee, Kyung-Tai Kim, Jiyoung Yoon, Jawoon Yi, Young Bin Hong, Byung-Ok Choi, Yoichi Kosodo, et al.,NucleoSpin RNA XS,MACHEREY-NAGEL,Cat# 740902.50, Commercial assay or kit,pGEM-T Easy Vector Systems,Promega,Cat# A1360, Commercial assay or kit,DiaStar OneStep RT-PCR kit,Solgent,Cat# DR61-K050, Commercial assay or kit,DIG RNA Labeling Mix

(6) Next generation marker-based vector concepts for rapid and unambiguous identification of single and double homozygous transgenic organismsBiology OpenOctober 15, 2023Frederic Strobl, Julia Ratke, Franziska Krämer, Ana Utta, Sigrun Becker, Ernst H. K. Stelzerinformation In this study, 20 vectors (Fig. S2 and Table S9) plus the commercial pGEM-T Easy subcloning vector (A1360, Promega) were used. Seven have been previously described, one was ordered as a gene synthesis plasmid,

(7) Regulation of SPDEF expression by DNA methylation in advanced prostate cancerFront Endocrinol (Lausanne)October 11, 2023Mousa Vatanmakanian, Joshua J. Steffan, Sweaty Koul, Augusto C. Ochoa, Lakshmi S. Chaturvedi, Hari K. Koulthirty nanograms of the resulting products were cloned into the pGEM®-T Easy Vector System (Promega, # A1360). The ligation reaction was incubated overnight at 4°C and then transformed to TOP-10 competent E. coli

(8) Comparison of plasma mitochondrial DNA copy number in asymptomatic and symptomatic COVID-19 patientsFrontiers in MicrobiologyOctober 6, 2023Shahrzad Shoraka, Seyed Reza Mohebbi, Seyed Masoud Hosseini, Mohammad Reza Zali(nuclear DNA). Each PCR product was inserted into a pGEM®-T Easy Vector System I (Promega, Cat. No. A1360) following the manufacturer’s protocol. Competent E. coli DH5α was chemically transformed with each

(9) Affinity hierarchies and amphiphilic proteins underlie the co-assembly of nucleolar and heterochromatin condensatesbioRxivSeptember 30, 2023Srivarsha Rajshekar, Omar Adame-Arana, Gaurav Bajpai, Kyle Lin, Serafin Colmenares, Samuel Safran, Gary H KarpenMulticolor Kit,",Thermo Fisher,F32951 Alexa Fluor™ dye combination,, pGEM T-Easy Cloning Kit,Promega,A1360 Recombinant DNA,, pGEM-rDNA,This study,N/A FISH Probes,, 359bp,This study,N/A 1.686,This study,N/A AAGAG,This

(10) Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectorsNature GeneticsSeptember 1, 2023Xiao Lin, Yuxin Jia, Robert Heal, Maxim Prokchorchik, Maria Sindalovskaya, Andrea Olave-Achury, Moffat Makechemu, Sebastian Fairhead, Azka Noureen, Jung Heo, et al.genes. Amplicons from the target genes were sub-cloned into the pGEM-Teasy TA cloning vector (Promega, A1360) or pICSL86977 for sequencing. The sequencing data were analyzed in Geneious (v10.2.6). Plant growth

(11) IF1 promotes oligomeric assemblies of sluggish ATP synthase and outlines the heterogeneity of the mitochondrial membrane potentialCommun BiolAugust 12, 2023Inés Romero-Carramiñana, Pau B. Esparza-Moltó, Sonia Domínguez-Zorita, Cristina Nuevo-Tapioles, José M. Cuezvarespectively. The resulting product was purified and first cloned into pGEM-T easy vector (Promega, A1360) and after subcloned into pEGFP_C3 (Clontech, 6082-1) in the XhoI and ApaI restriction sites. The resulting

(12) Mitochondrial haplotype mutation alleviates respiratory defect of MELAS by restoring taurine modification in tRNA with 3243A > G mutationNucleic Acids ResearchAugust 11, 2023Saori Ueda, Mikako Yagi, Ena Tomoda, Shinya Matsumoto, Yasushi Ueyanagi, Yura Do, Daiki Setoyama, Yuichi Matsushima, Asuteka Nagao, Tsutomu Suzuki, et al.mt tRNALeu(UUR) sequences were cloned by inserting into pGEM®-T Easy Vector (Promega, Madison, WI, A1360). Each plasmid was extracted using the Wizard® Plus SV Minipreps DNA Purification Systems (Promega,

(13) NvPrdm14d-expressing neural progenitor cells contribute to non-ectodermal neurogenesis in Nematostella vectensisNat CommunAugust 10, 2023Quentin I. B. Lemaître, Natascha Bartsch, Ian U. Kouzel, Henriette Busengdal, Gemma Sian Richards, Patrick R. H. Steinmetz, Fabian RentzschSupplementary Table 2. The cDNA of NvPrdm14 paralogs were cloned into the pGEM®-T Easy vector system (Promega, A1360). NvAtonal/neuroD (NVE18219, v1g220027), NvAshD (NVE2444, v1g210540) and NvPIT1 (NVE1681, v1g112929)

(14) Fucosylated glycoproteins and fucosylated glycolipids play opposing roles in cholera intoxicationbioRxivAugust 3, 2023Atossa C. Ghorashi, Andrew Boucher, Stephanie A. Archer-Hartmann, Nathan B. Murray, Rohit Sai Reddy Konada, Xunzhi Zhang, Chao Xing, Parastoo Azadi, Ulf Yrlid, Jennifer J. Kohlerinstructions. Purified DNA was ligated into a pGEM-T Easy pre-linearized vector (Promega, catalog no. A1360) and transformed into DH5α competent cells. Plasmids were extracted from single colonies and then submitted

(15) ZmmiR169q/ZmNF-YA8 is a module that homeostatically regulates primary root growth and salt tolerance in maizeFrontiers in Plant ScienceJune 28, 2023Lijuan Xing, Lan Zhang, Hongyan Zheng, Zhuoxia Zhang, Yanzhong Luo, Yuan Liu, Lei Wangthe coding region of ZmNF-YA8, was PCR amplified and cloned into the Promega pGEM-T vector (Cat No. A1360, Promega, Madison, WI, USA). Following sequence verification, PGEM-T-ZmNF-YA8 was linearized with SphI

(16) CRISPR/Cas9-based split homing gene drive targeting doublesex for population suppression of the global fruit pest Drosophila suzukiiProceedings of the National Academy of SciencesJune 20, 2023Amarish K. Yadav, Cole Butler, Akihiko Yamamoto, Anandrao A. Patil, Alun L. Lloyd, Maxwell J. Scott(NEB, #M0482S). RT-PCR products were purified and cloned into the pGem-T easy vector (Promega, Cat#A1360) followed by Sanger sequencing (SI Appendix, Table S8). 3′ RACE-PCR on RNA from 3A3, 1C2, and 23AI1SA

(17) Synthetic miR-21 decoy circularized by tRNA splicing mechanism inhibited tumorigenesis in glioblastoma in vitro and in vivo modelsMolecular Therapy - Nucleic AcidsJune 13, 2023Hadi Bayat, Mohammad Hossein Pourgholami, Saeid Rahmani, Safura Pournajaf, Seyed Javad MowlaFidelity Enzyme (Abbexa; Cat. #abx071010), TA-cloned into pGEM-T Easy Vector Systems (Promega; Cat. #A1360), and validated by performing Sanger sequencing using the Sanger Sequencing 3500 Dx Genetic Analyzer

(18) Unveiling Lathyrus aphaca L. as a Newly Identified Host for Begomovirus Infection: A Comprehensive StudyGenes (Basel)June 3, 2023Tehmina Bahar, Fasiha Qurashi, Muhammad Saleem Haider, Murad Ali Rahat, Fazal Akbar, Muhammad Israr, Ahmad Ali, Zahid Ullah, Fazal Ullah, Mohamed A. El-Sheikh, et al.(Thermoscientific InsTAclone PCR replicating kit)/pGEM-T Easy Vector PCR replicating kit (Promega Cat. # A1360). Entire amount of ligation combination: (based on the duration of the DNA portion of 200 to 550 ng

(19) Substitution of PINK1 Gly411 modulates substrate receptivity and turnoverAutophagyJune 1, 2023Fiesel FC, Fričová D, Hayes CS, Coban MA, Hudec R, Bredenberg JM, Broadway BJ, Markham BN, Yan T, Boneski PK, et al.DNA of positive candidates with PCR and cloned the PCR products into a pGEM T Easy vector (Promega, A1360) to confirm that all alleles had been edited correctly. After confirming on-target editing, we also

(20) Segmental expression of two ecdysone pathway genes during embryogenesis of hemimetabolous insectsDevelopmental BiologyJune 1, 2023Judith Wexler, Leslie Pick, Ariel ChipmanMEGAScript kit protocol. As a negative control for the dsRNA, sequence from Promega’s pGEM vector (A1360) between the M13F and M13R primers was amplified, and then SP6 and T7 polymerases were used to transcribe

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12/12/2022Great Kit for Cloning Work Wei Yang SitThis kit is an easy to use system to help users to clone PCR products generated by certain thermostable polymerases.
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6/11/2015Vector for TA cloning sndp kmrWe use this kit for cloning genes. By using this kit, we can select specific pairs of restriction enzyme sites at both the 5' and 3' ends of the desired gene to clone into a vector of interest. Blue ... Read Review