FastStart Universal SYBR Green Master (Rox) from Roche

Product Specs
ItemFastStart Universal SYBR Green Master (Rox)
CompanyRoche
Price Get Pricing Get Quote for this Product
Catalog Number04913914001
Quantity10 x 5 mL (for 2,000 reactions of 50 µL final reaction volume)

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

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Website: sequencing.roche.com

(1) Modeling RET-Rearranged Non-Small Cell Lung Cancer (NSCLC): Generation of Lung Progenitor Cells (LPCs) from Patient-Derived Induced Pluripotent Stem Cells (iPSCs)CellsDecember 15, 2023Paul Marcoux, Jin Wook Hwang, Christophe Desterke, Jusuf Imeri, Annelise Bennaceur-Griscelli, Ali G. Turhanfluorescence thermocycler (ThermoFisher Scientific QuantStudio 3TM) with FastStart Universal SYBR Green (04913914001, Roche, Vilnius, Lithuania) DNA dye. The primer sequences used for qRT-PCR are listed in the Supplementary

(2) Jianpi Yangxue Qufeng compound alleviates atopic dermatitis via TLR4/MyD88/NF-κB signaling pathwayHeliyonDecember 3, 2023Xuesong Yang, Zhimin Wang, Hong Huang, Guangyun Luo, Lin Cong, Jianting Yang, Jianzhou YeThe synthesized cDNA was amplified by Q-RT-PCR with 2 × FastStart Universal SYBR Green Master (ROX; 04913914001, Roche) using Agilent Mx3000P. β-actin was used as the control standard, and the mRNA levels were calculated

(3) MircroRNA-92b as a negative regulator of the TGF-β signaling by targeting the type I receptoriScienceNovember 17, 2023Shu Yang, Kewei Jiang, Lixing Li, Jiaqing Xiang, Yanchun Li, Lin Kang, Guangyan Yang, Zhen LiangKit,Qiagen,Cat#217061 Universal cDNA synthesis kit,Roche,Cat# 11483188001 SYBR Green I master,Roche,Cat#04913914001 iScript cDNA Synthesis Kit,Bio-Rad,Cat#1708890 Immunohistochemistry kit,ZSGB-BIO,Cat#PV9001; PV9002;

(4) miR‐221‐3p targets Ang‐2 to inhibit the transformation of HCMECs to tip cellsJournal of Cellular and Molecular MedicineNovember 1, 2023Peng Yang, Qing Yang, Yiheng Yang, Qingshan Tian, Zhenzhong Zheng30 s, and extension at 72°C for 20 s (FastStart Universal SYBR Green Master (Rox), Roche, Cat. No. 04913914001). The quality of PCR products was evaluated by generating a melting curve, which was also used to verify

(5) CGK733 alleviates ovariectomy-induced bone loss through blocking RANKL-mediated Ca2+ oscillations and NF-κB/MAPK signaling pathwaysiScienceOctober 20, 2023Minglian Xu, Dezhi Song, Xiaoxiao Xie, Yiwu Qin, Jian Huang, Chaofeng Wang, Junchun Chen, Yuangang Su, Jiake Xu, Jinmin Zhao, et al.X-100,Solarbio,T8200 DAPI,Solarbio,C0065 Trizol,Thermo Fisher Scientific,15596018 SYBR Green Master,Roche,04913914001 "Fluo-4, AM",Thermo Fisher Scientific,F14201 RIPA,Sigma-Aldrich,R0010 Loading buffer,Beyotime,P0015L DMEM,Thermo

(6) Lack of thyroid hormone receptor beta is not detrimental for non-alcoholic steatohepatitis progressioniScienceOctober 20, 2023Nuria Lopez-Alcantara, Rebecca Oelkrug, Sarah Christine Sentis, Henriette Kirchner, Jens MittagCocktail,Roche,Cat#5892970001 D-(+)-Glucose,Sigma-Aldrich,Cat#G7021 Direct Red 80,Sigma-Aldrich,Cat#365548 FastStart Universal SYBR Green Master Mix (+ROX),Roche,Cat# 04913914001 Histoplast Paraffin Wax,Thermo Fisher Scientific (Thermo Scientific),Cat#6774060 Insulin (NovoRapid

(7) Astragaloside IV derivative HHQ16 ameliorates infarction-induced hypertrophy and heart failure through degradation of lncRNA4012/9456Signal Transduction and Targeted TherapyOctober 19, 2023Jingjing Wan, Zhen Zhang, Chennan Wu, Saisai Tian, Yibei Zang, Ge Jin, Qingyan Sun, Pin Wang, Xin Luan, Yili Yang, et al.quantitative PCR analysis was performed using FastStart Universal SYBR Green Master kits (Roche, cat. no. 04913914001). Gene expression levels were normalized to the control gene Gapdh. The primer sequences for the genes

(8) Exo-miR-1290-induced by COX-2 overexpression promotes cancer-associated fibroblasts activation and tumor progression by CUL3-Nrf2 pathway in lung adenocarcinomaCell Communication and SignalingSeptember 18, 2023Xiaoming Bai, Jiaofang Shao, Tinghong Duan, Xue Liu, Min Wang, Xuanya Li, Qiang You, Zhiyuan Zhang, Jinshun Panreal-time PCR analysis was performed using the Power SYBR Green PCR master mix (Roche Diagnostics, #04913914001, Indianapolis, IN, USA). All the miRNAs RT and PCR primers were obtained from Ribobio Biotechnology

(9) IL-24 is the key effector of Th9 cell-mediated tumor immunotherapyiScienceSeptember 15, 2023Jintong Chen, Yunwei Zhang, Hua Zhang, Mingyue Zhang, He Dong, Tianxue Qin, Sujun Gao, Siqing WangFirst-Strand cDNA Synthesis SuperMix,TransGen Biotech,Cat#AT341-02 FastStart Universal SYBR Green Master,Roche,Cat#04913914001 RIPA Buffer,CST,Cat#9806 Protease/Phosphatase inhibitor cocktail,CST,Cat#5872 ,, Critical commercial

(10) GAPDH: A common housekeeping gene with an oncogenic role in pan-cancerComputational and Structural Biotechnology JournalAugust 9, 2023Jin Wang, Xueting Yu, Xiyuan Cao, Lirong Tan, Beibei Jia, Rui Chen, Jianxiang Liinstructions. qPCR was performed using the FastStart Universal SYBR Green Master (ROX) Kit (Cat. No. 04913914001. Roche Diagnostics, Switzerland) on the QuantStudioTM 6 Flex RT-qPCR System (Applied Biosystems; Thermo

(11) ACSS2-dependent histone acetylation improves cognition in mouse model of Alzheimer’s diseaseMolecular NeurodegenerationJuly 12, 2023Yingbin Lin, Anlan Lin, Lili Cai, Weibin Huang, Shanzhi Yan, Yuanxiang Wei, Xinglin Ruan, Wenting Fang, Xiaoman Dai, Jinbo Cheng, et al.Then, the cDNA was subjected to real-time quantitative PCR analysis with a SYBR Green Master (Roche, 04913914001) and specific primers. PCR primer sequences were shown in Appendix Table S3. Western blotting Brain

(12) A bioinformatics analysis and an experimental validation of the hypoxia-related prognostic modelJournal of Gastrointestinal OncologyJune 30, 2023Lei Zhou, Weigang Zhang, Haoxiang Ni, Jin Liu, Hui Sun, Zhanwen Liang, Ruoqin Wang, Xiaofeng Xue, Kai Chen, Wei Liincluded the corresponding cDNA, forward primer, reverse primer, and SYBR Green PCR master mix (Cat: 04913914001, Roche). The primer sequences are summarized in Table S2. Wound healing assay Wound healing assays

(13) Prognostic evaluation of pancreatic cancer based on the model of chemo-radiotherapy resistance-related genesJournal of Gastrointestinal OncologyJune 30, 2023Hui Sun, Weigang Zhang, Yunqian Chu, Lei Zhou, Feiran Gong, Wei Li, Kai Chencontained reverse and forward primers, corresponding cDNA, and the SYBR Green PCR master mix (Roche, Cat: 04913914001). The data were analyzed using the 2-ΔΔCt method, with β-Actin gene expression serving as the internal

(14) Perilipin5 protects against non-alcoholic steatohepatitis by increasing 11-Dodecenoic acid and inhibiting the occurrence of ferroptosisNutrition & metabolismJune 22, 2023Xu X, Qiu J, Li X, Chen J, Li Y, Huang X, Zang S, Ma X, Liu J.RT reagent Kit (TaKaRa, RR047A). The RT-PCR experiment was carried out using SYBR green Premix kit (04913914001, Roche) next. Normalize gene expression levels by using β-actin or GAPDH as an internal reference. Additional

(15) Bioluminescence Contributes to the Adaptation of Deep-Sea Bacterium Photobacterium phosphoreum ANT-2200 to High Hydrostatic PressureMicroorganismsMay 23, 2023Xu-Chong Bao, Hong-Zhi Tang, Xue-Gong Li, An-Qi Li, Xiao-Qing Qi, Deng-Hui Li, Shan-Shan Liu, Long-Fei Wu, Wei-Jia ZhangµL contained FastStart Universal SYBR Green Master (ROX) (Roche, Mannheim Germany, catalogue number 04913914001), 0.6 µL of each primer (10 mM) and 1 µL of 10-times diluted cDNA as template. The thermal cycling protocol

(16) Chronic acidosis rewires cancer cell metabolism through PPARα signalingInternational journal of cancerApril 15, 2023Rolver MG, Holland LKK, Ponniah M, Prasad NS, Yao J, Schnipper J, Kramer S, Elingaard-Larsen L, Pedraz-Cuesta E, Liu B, et al.Transcriptase (Invitrogen, #18080044). cDNA was amplified in triplicates by qPCR utilizing SYBR Green (Roche, #04913914001) in an ABI7900 qPCR machine with the steps: 95°C for 10 min, 40 × [95°C for 30 s, 55‐63°C (depending

(17) Cycloastragenol: A Novel Senolytic Agent That Induces Senescent Cell Apoptosis and Restores Physical Function in TBI-Aged MiceInternational Journal of Molecular SciencesMarch 31, 2023Yanghuan Zhang, Dongxiao Gao, Yang Yuan, Runzi Zheng, Manting Sun, Shuting Jia, Jing LiuPromega, Madison, WI, USA). qRT-PCR was performed in a Real-Time PCR System 7300 using SYBR Mix (Cat. 04913914001, Roche, Basel, Switzerland), following a program consisting of 95 °C for 10 min, then 40 cycles of 95

(18) Biphasic expression of thyroid hormone receptor TRβ1 in mammalian retina and anterior ocular tissuesFront Endocrinol (Lausanne)March 23, 2023Lily Ng, Hong Liu, Ye Liu, Douglas Forrest#18091050). The qPCR reaction was performed with FastStart Universal SYBR Green Master-Mix (Roche; Cat #04913914001) on a StepOne or QuantStudio 3 instrument with analysis using software provided by the manufacturer

(19) Effects of Pendrin Protein in Nasal Epithelial Cells on Mucin Production in the Context of Type 2 InflammationJournal of Personalized MedicineMarch 10, 2023Nongping Zhong, Honghui Ai, Wei Zhong, Xiaoyan Huang, Kai Wang, Qing Luo, Jieqing Yu(#K1622; Thermo Fisher Scientific, Waltham, MA, USA) and a Fast Start Universal SYBR Green Master Kit (04913914001; Roche Holding AG, Basel, Switzerland) for quantitative PCR. The primers for PCR were as follows: β-actin,

(20) Overexpression of miR-92a attenuates kidney ischemia–reperfusion injury and improves kidney preservation by inhibiting MEK4/JNK1-related autophagyCellular & Molecular Biology LettersMarch 8, 2023Ming Ma, Hui Li, Saifu Yin, Tao Lin, Turun SongThermo Fisher Scientific Inc.). Real-time-qPCR was performed using FastStart Universal SYBR Green Master (ROX) (04913914001, Roche). All of the procedures were performed according to the kit instructions. U6 messenger RNA (mRNA)

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3/17/2016SYBR Green Master real time PCR Lin ChenSYBR Green Master mix is an ideal tool for real time PCR. It is easy to use, highly efficient, and much less expensive than ABI's similar product.

FastStart Universal SYBR Green Master (Rox) from Roche

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FastStart Universal SYBR Green Master (Rox) from Roche

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