FastStart SYBR Green Master from Roche

Product Specs
ItemFastStart SYBR Green Master
CompanyRoche
Price Get Pricing Get Quote for this Product
Catalog Number04673492001
Quantity50 ml (10 x 5 ml)

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Transcriptional analysis of the expression and prognostic value of lipid droplet-localized proteins in hepatocellular carcinomaBMC cancerJuly 18, 2023Zhang Y, Liang X, Lian Q, Liu L, Zhang B, Dong Z, Liu K.and the resulting cDNAs were subjected to qRT-PCR analysis with FastStart SYBR Green Master (Roche, 04673484001, Germany) in LightCycler 96 Real-Time PCR system (Roche, D10013, Germany). The amplification primers

(2) Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and miceInternational journal of biological sciencesJanuary 1, 2023Hu M, Bodnar B, Zhang Y, Xie F, Li F, Li S, Zhao J, Zhao R, Gedupoori N, Mo Y, et al.using Primer Script II Reverse. RT-qPCR was performed with FastStart SYBR Green Master (Roche, cat. no. 04673484001) using the CFX96 Real-Time PCR System (Bio-Rad). All primers used for RT-qPCR spanned an exon-exon junction

(3) ROCK1 mechano-signaling dependency of human malignancies driven by TEAD/YAP activationNature CommunicationsDecember 1, 2022Davide Esposito, Ila Pant, Yao Shen, Rui F. Qiao, Xiaobao Yang, Yiyang Bai, Jian Jin, Poulikos I. Poulikakos, Stuart A. Aaronsonthe ViiA™ 7 Real-Time PCR System (Life Technologies) and the FastStart SYBR Green Master mix (Roche, 04673492001). PCR was performed in 384 well plates with 10 µl under the following conditions: 95 °C for 15 min,

(4) Evaluation of a library of loxP variants with a wide range of recombination efficiencies by CrePLOS ONEOctober 21, 2022Yuji Yamauchi, Hidenori Matsukura, Keisuke Motone, Mitsuyoshi Ueda, Wataru Aokiof distilled water, 25 μL of FastStart SYBR Green Master (without ROX) (Roche, Basel, Switzerland, 04673484001), 1.5 μL of 10 pmol/μL forward primer, 1.5 μL of 10 pmol/μL reverse primer, and 5 μL of the template

(5) Differential expression of Ago2â€mediated microRNA signaling in adipose tissue is associated with foodâ€induced obesityFEBS Open BioOctober 1, 2022Hansi Zhang, Liang Qiao, Xiaoxuan Liu, Xiaojing Han, Jing Kang, Yanli Liu, Juntang Lin, Xin Yan(mmu477814_mir) and U6 snRNA (001973). qPCR of mRNAs was detected using FastStart SYBR Green Master (04673484001, Roche, Shanghai, China) and normalized to β‐actin in StepOne™ Real‐Time PCR Systems (Applied Biosystems).

(6) Dynamic interplay of two molecular switches enabled by the MEK1/2-ERK1/2 and IL-6-STAT3 signaling axes controls epithelial cell migration in response to growth factorsThe Journal of biological chemistryOctober 1, 2021Qin L, Cao X, Kaneko T, Voss C, Liu X, Wang G, Li SS.following the manufacturer's protocol. After mixing the extracted RNA with FastStart SYBR Green Master (04673492001; Roche) and specific primers, the RT-qPCR was performed on a Stratagene Mx3005P QPCR System (QIAGEN-SABiosciences).

(7) High B3GALT5 expression confers poor clinical outcome and contributes to tumor progression and metastasis in breast cancerBreast cancer research : BCRJanuary 7, 2021Liao YM, Wang YH, Hung JT, Lin YJ, Huang YL, Liao GS, Hsu YL, Wu JC, Yu AL.5′-TGGGCGGTGTAGAATCAGAGTC-3′. The Q-PCR assay was performed with Brilliant SYBR Green QPCR Master Mix (#04673484001, Roche, Basel, Switzerland) by 7500 Fast real-time PCR system (ThermoFisher, CA, USA). The relative

(8) GPER‐induced signaling is essential for the survival of breast cancer stem cellsInternational Journal of CancerMarch 15, 2020Yu‐Tzu Chan et al.TaqMan Reverse Transcription Reagents (Cat. #N8080234, Applied Biosystems, Foster City, CA). FastStart SYBR Green Master (Cat. #04673484001, Roche Applied Science, Indianapolis, IN) was used for RT‐qPCR and analyzed using a 7300 Sequence Detection

(9) Crosstalk with lung epithelial cells regulates Sfrp2-mediated latency in breast cancer disseminationNature cell biologyMarch 1, 2020Montagner M, Bhome R, Hooper S, Chakravarty P, Qin X, Sufi J, Bhargava A, Ratcliffe CDH, Naito Y, Pocaterra A, et al.Real-Time PCR System (Thermo Fisher Scientific) with a FastStart SYBR 548 Green Master Mix (Roche 04673492001). 549 For RNA sequencing experiments of disseminated breast cancer cells in vivo, 1x106 550 D2.A1-eGFP

(10) FMRP has a cell-type-specific role in CA1 pyramidal neurons to regulate autism-related transcripts and circadian memoryeLifeDecember 20, 2019Kirsty Sawicka, Caryn R Hale, Christopher Y Park, John J Fak, Jodi E Gresack, Sarah J Van Driesche, Jin Joo Kang, Jennifer C Darnell, Robert B Darnell,iScript cDNA Synthesis Kit,Bio-Rad,Cat# 1708891, Commercial assay or kit,FastStart SYBR Green Master,Roche,Cat# 04673484001,

(11) In silico prediction of housekeeping long intergenic non-coding RNAs reveals HKlincR1 as an essential player in lung cancer cell survivalScientific ReportsDecember 1, 2019Danish Memon, Jing Bi, Crispin J. MillerExpressions were quantified by semiquantitative and quantitative PCR (Fast start SYBR green, Roche, 04673484001) and shown as normalised expression relative to beta-actin. Error bars represent the standard deviations

(12) AGO CLIP Reveals an Activated Network for Acute Regulation of Brain Glutamate Homeostasis in Ischemic StrokeCell reportsJuly 23, 2019Kobayashi M, Benakis C, Anderson C, Moore MJ, Poon C, Uekawa K, Dyke JP, Fak JJ, Mele A, Park CY, et al.v2,Illumina,Cat# RSS-122–2001 miScript II RT Kit,Qiagen,Cat# 218160 FastStart SYBR Green Master,Roche,Cat# 04673492001 Dual-Glo Luciferase Assay System,Promega,Cat# E2920 In-Fusion HD Cloning Plus CE kit,Takara Bio,Cat#

(13) Activated kinase screening identifies the IKBKE oncogene as a positive regulator of autophagyAutophagyFebruary 1, 2019Leonardi M, Perna E, Tronnolone S, Colecchia D, Chiariello M.Real time PCR (RT-PCR) was performed with the FastStart SYBR Green Master Mix (Roche Applied Science, 04673484001) on a Rotor-Gene 6000 RT-PCR system (Corbett Life Science, Sydney, Australia). Cell proliferation assays MDA-MB-231,

(14) Complement C3aR Inactivation Attenuates Tau Pathology and Reverses an Immune Network Deregulated in Tauopathy Models and Alzheimer's DiseaseNeuronDecember 19, 2018Litvinchuk A, Wan YW, Swartzlander DB, Chen F, Cole A, Propson NE, Wang Q, Zhang B, Liu Z, Zheng H.Superscript IV First Strand synthesis system (Invitrogen, 18091050). The qPCR analyses were performed using SYBR Green PCR master mix (Roche, 04673484001). Primer sequences can be found in Table S1. Western blot analysis. Tissues or cells were extracted

(15) Development of versatile non-homologous end joining-based knock-in module for genome editingScientific reportsJanuary 12, 2018Sawatsubashi S, Joko Y, Fukumoto S, Matsumoto T, Sugano SS.performed using a LightCycler 96 (Roche. Basel, Switzerland) with FastStart Essential DNA Green Master (04673492001; Roche). Results were calculated as mean ± S.D. from at least three independent experiments. Primer

(16) Homeodomain interacting protein kinase promotes tumorigenesis and metastatic cell behaviorDisease Models & MechanismsJanuary 1, 2018Jessica A. Blaquiere, Kenneth Kin Lam Wong, Stephen D. Kinsey, Jin Wu, Esther M. VerheyenFirst Strand cDNA Synthesis Kit (TaKaRa; 6110A). qPCR was performed using FastStart SYBR Green Master (Roche; 04673484001) on StepOne real-time PCR (ABI). HPRT was used as a housekeeping gene control. Primers used were as

(17) Sequestosome1/p62 protects mouse embryonic fibroblasts against low-dose methylercury-induced cytotoxicity and is involved in clearance of ubiquitinated proteinsScientific ReportsDecember 1, 2017Yasukazu Takanezawa, Ryosuke Nakamura, Ryohei Harada, Yuka Sone, Shimpei Uraguchi, Masako Kiyono(Thermo Fisher Scientific, 4368814). The RT-qPCR was performed using FastStart SYBR Green Master (Roche, Indianapolis, IN, 04673484001) in a CFX-96 thermal cycler system (Bio-Rad) in accordance with manufacturer’s protocol. Triplicate

(18) Tumor suppressor NPRL2 induces ROS production and DNA damage responseScientific ReportsDecember 1, 2017Yinxing Ma, Licia Silveri, John LaCava, Svetlana Dokudovskayacocktail (04693159001), Phosphatase inhibitor cocktail (04 906 837 001), Fast start SYBR Green Master (04673484001) and XTT assay kit (11465015001) were from Roche. JetPEI DNA transfection reagent (101-10N) was from

(19) Systematic identification of regulatory variants associated with cancer riskGenome BiologyDecember 1, 2017Song Liu, Yuwen Liu, Qin Zhang, Jiayu Wu, Junbo Liang, Shan Yu, Gong-Hong Wei, Kevin P. White, Xiaoyue Wangprimer. cDNA (1 μL) was used as a template for qPCR analyses with FastStart SYBR Green MasterMix (Roche, 04673484001) with primers listed below. Relative gene expression was calculated using the ∆∆Ct method and the expression

(20) β-catenin activity in late hypertrophic chondrocytes locally orchestrates osteoblastogenesis and osteoclastogenesisDevelopmentOctober 15, 2016Astrid Houben, Daniela Kostanova-Poliakova, Martina Weissenböck, Julian Graf, Stefan Teufel, Klaus von der Mark, Christine Hartmannanalysis First-strand cDNA (2-3 µl of 1:10 diluted) was mixed with either Fast Start SBG Master Mix (Roche, #04673484001) or SYBR Premix Ex Taq II (TaKaRa, #RR820Q). Gene expression was monitored using a Bio-Rad CFX96 cycler.

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FastStart SYBR Green Master from Roche

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FastStart SYBR Green Master from Roche

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